Ebook: Practical Cell Culture Techniques

Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

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Practical Cell Culture Techniques focuses on the advantages of new tissue culture techniques that enable close and detailed study of many nerve cell types. Neuroscience is one of the key areas whose rapid development has been driven by the proliferation of cell culture methodology; some areas of neuroscience, such as glial cell biology and function, now depend almost entirely on cell culture-based research projects.

This latest volume of Humana's renowned Neuromethods series provides practical treatment of the new, powerful methodologies and results including: cell culture techniques specifically designed for the novice • the environmental factors that play a role in tissue culturing • cell markers • nutrition requirements of the different cell types • three-dimensional organotypic slice explant cultures • neuronal cultures • cultures of astrocytes, oligodendrocytes, cerebral capillary endothelium, and other cell types.

Boulton, Baker, and Walz's new volume on Practical Cell Culture Techniques is sure to be a major resource for everyone-whether expert or novice-involved in studying the central nervous system on the molecular level.

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Practical Cell Culture Techniques focuses on the advantages of new tissue culture techniques that enable close and detailed study of many nerve cell types. Neuroscience is one of the key areas whose rapid development has been driven by the proliferation of cell culture methodology; some areas of neuroscience, such as glial cell biology and function, now depend almost entirely on cell culture-based research projects.

This latest volume of Humana's renowned Neuromethods series provides practical treatment of the new, powerful methodologies and results including: cell culture techniques specifically designed for the novice • the environmental factors that play a role in tissue culturing • cell markers • nutrition requirements of the different cell types • three-dimensional organotypic slice explant cultures • neuronal cultures • cultures of astrocytes, oligodendrocytes, cerebral capillary endothelium, and other cell types.

Boulton, Baker, and Walz's new volume on Practical Cell Culture Techniques is sure to be a major resource for everyone-whether expert or novice-involved in studying the central nervous system on the molecular level.
Content:
Front Matter....Pages i-xix
Back Matter....Pages 1-20
....Pages 21-62