Ebook: Patch-Clamp Applications and Protocols

E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.

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Patch-Clamp Applications and Protocols presents the broadest coverage of techniques for measuring and recording directly activity within a cell. The first part of the book offers modern developments associated with the technology of path-clamp electrodes, of cell-free ion-channel recording, and of the whole-cell patch clamp technique.

Chapters on such recent offspring of the technique as the concentration clamp technique, the pressure clap method, and the perfusion of patch clamp electrodes are contributed by authors intimately involved with their development. Also covered is the loose patch-clamp technique, single-channel recording, the perforated patch-clamp technique, molecular biological aspects of the technique, and the use of the technique in brain slices.

Every scientist working with path-clamp methods will find this protocols manual invaluable to their continued use and development of this powerful technique.

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Patch-Clamp Applications and Protocols presents the broadest coverage of techniques for measuring and recording directly activity within a cell. The first part of the book offers modern developments associated with the technology of path-clamp electrodes, of cell-free ion-channel recording, and of the whole-cell patch clamp technique.

Chapters on such recent offspring of the technique as the concentration clamp technique, the pressure clap method, and the perfusion of patch clamp electrodes are contributed by authors intimately involved with their development. Also covered is the loose patch-clamp technique, single-channel recording, the perforated patch-clamp technique, molecular biological aspects of the technique, and the use of the technique in brain slices.

Every scientist working with path-clamp methods will find this protocols manual invaluable to their continued use and development of this powerful technique.
Content:
Front Matter....Pages i-xiv
Back Matter....Pages 1-36
....Pages 37-73