Ebook: Practical HPLC Method Development, Second Edition

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.Content:
Chapter 1 Getting Started (pages 1–20):
Chapter 2 Basics of Separation (pages 21–58):
Chapter 3 Detection Sensitivity and Selectivity (pages 59–99):
Chapter 4 Sample Preparation (pages 100–173):
Chapter 5 The Column (pages 174–232):
Chapter 6 Non?Ionic Samples: Reversed? and Normal?Phase HPLC (pages 233–291):
Chapter 7 Ionic Samples: Reversed?Phase, Ion?Pair, and Ion?Exchange HPLC (pages 292–349):
Chapter 8 Gradient Elution (pages 350–401):
Chapter 9 Systematic Approach to the Reversed?Phase Separation of Regular Samples (pages 402–438):
Chapter 10 Computer?Assisted Method Development (pages 439–478):
Chapter 11 Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds (pages 479–536):
Chapter 12 Chiral Separations (pages 537–615):
Chapter 13 Preparative HPLC Separation (pages 616–642):
Chapter 14 Quantitation (Including Trace Analysis) (pages 643–684):
Chapter 15 Completing the Method: Validation and Transfer (pages 685–713):