Ebook: Fluorescent Methods to Study Biological Membranes
- Tags: Physical Chemistry, Analytical Chemistry, Membrane Biology
- Series: Springer Series on Fluorescence 13
- Year: 2013
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.
Content:
Front Matter....Pages i-xiii
LAURDAN Fluorescence Properties in Membranes: A Journey from the Fluorometer to the Microscope....Pages 3-35
Application of NBD-Labeled Lipids in Membrane and Cell Biology....Pages 37-50
3-Hydroxychromone Probes Precisely Located and Oriented in Lipid Bilayers: A Toolkit for Biomembrane Research....Pages 51-69
Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and Microscopy....Pages 71-113
FRET Analysis of Protein-Lipid Interactions....Pages 115-140
Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift....Pages 141-159
Visual Discrimination of Membrane Domains in Live Cells by Widefield Microscopy....Pages 163-184
Quantitative Fluorescence Studies of Intracellular Sterol Transport and Distribution....Pages 185-213
Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)....Pages 215-240
Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions....Pages 241-269
Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length Scales....Pages 271-289
STED-FCS Nanoscopy of Membrane Dynamics....Pages 291-309
Imaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy....Pages 311-337
Near-Field Optical Nanoscopy of Biological Membranes....Pages 339-363
Unveiling Biophysical and Biological Properties of a Hypothetical Membrane Receptor by Exploiting Recent Imaging Advances....Pages 367-388
New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization....Pages 389-415
Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dynamics of the Serotonin1A Receptor....Pages 417-437
TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and Spectroscopy....Pages 439-455
Back Matter....Pages 479-485
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.
Content:
Front Matter....Pages i-xiii
LAURDAN Fluorescence Properties in Membranes: A Journey from the Fluorometer to the Microscope....Pages 3-35
Application of NBD-Labeled Lipids in Membrane and Cell Biology....Pages 37-50
3-Hydroxychromone Probes Precisely Located and Oriented in Lipid Bilayers: A Toolkit for Biomembrane Research....Pages 51-69
Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and Microscopy....Pages 71-113
FRET Analysis of Protein-Lipid Interactions....Pages 115-140
Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift....Pages 141-159
Visual Discrimination of Membrane Domains in Live Cells by Widefield Microscopy....Pages 163-184
Quantitative Fluorescence Studies of Intracellular Sterol Transport and Distribution....Pages 185-213
Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)....Pages 215-240
Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions....Pages 241-269
Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length Scales....Pages 271-289
STED-FCS Nanoscopy of Membrane Dynamics....Pages 291-309
Imaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy....Pages 311-337
Near-Field Optical Nanoscopy of Biological Membranes....Pages 339-363
Unveiling Biophysical and Biological Properties of a Hypothetical Membrane Receptor by Exploiting Recent Imaging Advances....Pages 367-388
New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization....Pages 389-415
Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dynamics of the Serotonin1A Receptor....Pages 417-437
TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and Spectroscopy....Pages 439-455
Back Matter....Pages 479-485
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