Ebook: Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

The 21st ESACT conference was held in the beautiful surroundings of the CityWest Hotel resort in Dublin, Ireland. For the first time in ESACT history the number of participants exceeded 900: a sign of the ever increasing importance of this area. The conference commenced on Sunday June 5th with two sets of parallel workshops on the subjects listed below. An additional workshop was held on Monday lunchtime of the conferenceProcess Analytical Technology (PAT), Quality by Design (QbD) and other recent regulatory developments.

2. Innovative media products for the 21^st century biopharmaceutical industry.

3. The impact of high titre media feed-streams on monoclonal antibody purification.

4. Advances in genomics and proteomics.

5. Stem Cell Technology: new developments and clinical applications.

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The 21st ESACT meeting – ‘Cellular Solutions for Clinical Challenges’ outlined the novel capabilities of animal cell technology to move towards the clinic. The highly successful meeting was attended by a record number of researchers and engineers interested in all aspects of animal cell technology. The meeting included sessions on Stem Cells, Tissue Engineering, Advances in Bioprocessing, Biotherapeutics, Novel Vaccines and Virology and Advances in Synthetic and Molecular Biology. The meeting was supported by an array of exceptional invited speakers from around the world whose contributions complemented the emerging technologies and the challenges facing the clinical application of animal cell culture and technologies. The proceedings here include the short papers, adding to the wealth of knowledge gained from previous ESACT meetings.

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The 21st ESACT meeting – ‘Cellular Solutions for Clinical Challenges’ outlined the novel capabilities of animal cell technology to move towards the clinic. The highly successful meeting was attended by a record number of researchers and engineers interested in all aspects of animal cell technology. The meeting included sessions on Stem Cells, Tissue Engineering, Advances in Bioprocessing, Biotherapeutics, Novel Vaccines and Virology and Advances in Synthetic and Molecular Biology. The meeting was supported by an array of exceptional invited speakers from around the world whose contributions complemented the emerging technologies and the challenges facing the clinical application of animal cell culture and technologies. The proceedings here include the short papers, adding to the wealth of knowledge gained from previous ESACT meetings.
Content:
Front Matter....Pages i-xliv
Front Matter....Pages 1-1
MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes....Pages 3-11
Are Clones Really Unstable?....Pages 13-18
An Optimised Transfection Platform for the Epi-CHO Transient Expression System in Serum-free Media....Pages 19-23
Using the “OMICS” Technologies as Complementary Tools to Study the Molecular Mechanisms Involved with the Adaptation of Myeloma Cell Line to Protein-Free Medium....Pages 25-29
Galectin-3 Over-Expression Enhances Survival and Recombinant Protein Expression in Mammalian Cells....Pages 31-35
Metabolomic Analysis of CHO Cultures with Different Growth Characteristics – Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells....Pages 37-41
Cell Lines in Four Weeks with the CEMAX® System....Pages 43-46
Analysis of Protein Expression via Alternate 3’ Untranslated Region (UTR) Signals Through the Use of Site Specific Recombination....Pages 47-51
A Case Study in Clone Screening: A Comprehensive Approach for a Product With High Projected Market Demand....Pages 53-56
An Improved Clone Selection Method....Pages 57-60
Dissecting the Mechanism of Action of BHRF1 for the Protection Against Apoptosis in MAb-Producing Cell Lines....Pages 61-65
Qualification of Bench and Pilot-Scale Bioreactors as Models of Commercial Systems....Pages 67-70
Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance....Pages 71-75
Implications of Vector Fragmentation and Initial Production Levels in Production Cell Line Development Using MTX....Pages 77-83
Characterizing the Expression Stability in Different Phenotypes of Recombinant NS0 Myeloma Cell Lines....Pages 85-90
A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells....Pages 91-94
Proteomic Profiling of Temperature-Shifted CHO Cells to Identify Genes that Impact on Both Cell Growth and Recombinant Protein Productivity in Suspension Culture....Pages 95-107
Stabilization of Protein Expression in Mammalian Cells Employing a Toxin/Antitoxin Based Strategy....Pages 109-113
Novel Cell Lines For Bioprocessing: Friend or Foe?....Pages 115-119
Transgene mRNA Levels and Stability are Key Factors to Enhance Transient Gene Expression in CHO DG44 Cells....Pages 121-124
Front Matter....Pages 1-1
High Cell Density Transient Gene Expression in HEK 293 EBNA Cells....Pages 125-128
Generation of High-Producing CHO Cell Lines by Piggybac Transposition....Pages 129-133
Cellular Proteins in Conditioned Medium Inhibit Polyethylenimine-Mediated Transfection of CHO Cells....Pages 135-138
A New Variant of the Affinity Matrix Method for Identification of High Producing Cells in Mammalian Cell Culture....Pages 139-142
Novel Strategies for Improving Cell Viability and Production Yield....Pages 143-145
Front Matter....Pages 147-147
Regulating in Vitro Motility of Human Mesenchymal Stem Cells with Macrophage Migration Inhibitory Factor (MIF) and a Small-Molecule MIF Antagonist....Pages 149-160
Impact of Growth Factors on the Proliferation of Ear Mesenchymal Stem Cells on Porous Microcarriers....Pages 161-176
Strategies for Pancreatic Differentiation of Pluripotent Stem Cells....Pages 177-187
Stem Cell Biology: New Applications for Studying Metabolic Diseases....Pages 189-203
Monitoring of Long-Term Cultivation and Osteogenic Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cell-Like Cultures....Pages 205-207
cGMP-Compliant Isolation, Expansion and Quality Testing of Human Bone Marrow-Derived Mesenchymal Stem Cells....Pages 209-211
Cancer Research, Molecular and Cell Biology: Look at the Nanotechnology, Based Nanoparticles for Diagnostics, and Therapy. Stem Cell for Treatment of Cancer Diseases....Pages 213-218
A Comparative Study of Suspension Cultivation Systems for the Expansion of Undifferentiated Mouse Embryonic Stem Cells....Pages 219-222
Front Matter....Pages 223-223
Human Endothelial Cell Lines with In Vivo Physiology....Pages 225-234
Quality Control and Cell Line Cross-Contamination: An Old Problem that Continues To Fester....Pages 235-239
Functional Characterisation of Human Hepatoma Cell Line HepG2 in 3D Culture....Pages 241-246
GFP-Expressing Bladder Fibroblasts for Applications in Tissue Engineering....Pages 247-250
3-Dimensional, Dynamic Cultivations of Human Umbilical Cord-Derived Cells....Pages 251-255
NIH/3T3 Feeder System to Engineer Corneal Epithelial Sheets with Enhanced Positive Progenitor Populations....Pages 257-260
NF-?B Signalling Pathway: Generation and Characterization of a Reporter Cell Line of Human Origin....Pages 261-263
Front Matter....Pages 223-223
Establishing Mammalian Production Cell Lines for Structural Biology by Site-Specific Recombination....Pages 265-268
Front Matter....Pages 269-269
Regulation of Transferrin Receptor and IGF-I Receptor Numbers at the Cell Surface Drives Growth and Productivity of Hybridoma Cells....Pages 271-283
Towards Enhancing Manufacturing Process Performance Through Multivariate Data Mining....Pages 285-294
Partial Replacement of Chemically Defined CHO Media with Plant-Derived Protein Hydrolysates....Pages 295-298
An Insight into the Physiology of Insect Cells: The Role of Energetic Metabolism on the Cell Density Effect....Pages 299-305
Design and Development of a High-Throughput Platform for Rapid Microbe Identification and Automatic Data Management....Pages 307-313
On-line Monitoring of the Live Cell Concentration in Disposable Bioreactors....Pages 315-318
The Challenge of Validating a Viable Biomass Probe for cGMP Processes....Pages 319-322
Study of the Effect of High pH and Alkali Addition in a Cultivation of Chinese Hamster Ovary Cell....Pages 323-326
Tuning of Dissolved Oxygen and pH PID Control Parameters in Large Scale Bioreactor by Lag Control....Pages 327-330
Kinetic Analysis of Recombinant BHK Roller Bottle Cultures....Pages 331-334
Manipulation of a Perfusion Process by Medium Optimization....Pages 335-340
Feed Flow Pulsation in the Separation of CHO Cells in Hydrocyclones: Effects of Pressure Drop and Pumphead Type on Separation Efficiency and Cell Viability....Pages 341-344
Development of a Biphasic Culture Process for Recombinant Protein Production in Human CAP Cells....Pages 345-349
Crossed Mixture Design and Artificial Neural Networks: An Efficient Approach to Cell Culture Medium Optimization....Pages 351-354
Improving Cell Culture Bioreactor Performance for Sensitive Cell Lines by Dynamic Membrane Aeration (DMA)....Pages 355-358
Evaluation of Disposable Bioreactor Design Compared to Reusable Stainless Steel Reactors....Pages 359-362
Utilising Scale Model Systems to Optimise Upstream Process Development....Pages 363-367
Human Platelet Lysates as a Serum Substitute in Cell Culture Media....Pages 369-373
4 g/L.day: Monoclonal Antibody Volumetric Productivity in the iCELLisTM Disposable Fixed-Bed Bioreactor....Pages 375-378
Front Matter....Pages 269-269
Optimisation of Cell Growth and Recombinant Protein Production in Small-Scale Culture Vessels by Using Optical Sensors for On-line Measurement of Dissolved Oxygen....Pages 379-382
A Protocol for Cell Detachment of Vero Cells Grown Under Fully Animal Component Free Conditions and on Cytodex 1 Microcarriers....Pages 383-386
Simulation and Optimization of Essential Amino Acids in Dynamic Mammalian Cell Culture....Pages 387-390
Biomass Sensors in iCELLis™ Fixed-Bed Reactors: Data on CHO and Vero Cells....Pages 391-394
On-line Monitoring: Animal Cell Cultivation in iCELLis™ Fixed-Bed Reactor Using Dielectric Measurements....Pages 395-399
Change of the Role for JAK/STAT3 in IL-6 Response During Adaptation of Hybridoma Cells to Serum-Free Media....Pages 401-403
Front Matter....Pages 405-405
Engineering Principles and Cell Culture Performance of Orbitally Shaken Cylindrical Bioreactors....Pages 407-412
Dynamic Ranking of Clones with Process Conditions Using a High-Throughput Micro-bioreactor Platform....Pages 413-416
Improved Cell Culture Surface for Biotechnological Research....Pages 417-420
Platform Validation of Dissolved Oxygen Ranges for Cell Culture Processes....Pages 421-424
Study of Growth Factors in Spent Medium for Better Mammalian Cell Culture....Pages 425-428
Process Improvements for Production of a Complex Glycosylated Fusion Protein which Delivers a Two-Fold Increase in Product Titre and Comparable Product Quality....Pages 429-431
Hydro-Kinetic Modelling of Animal Cell Response to Turbulent Mixing in Bioreactors....Pages 433-436
Near Infrared Spectroscopy as an In-Situ PAT Tool to Monitor Adherent VERO Cell Culture Processes....Pages 437-440
VERO Cell Metabolism in Animal Component-Free Media: Influence of Glucose and Glutamine Substitution....Pages 441-444
Metabolic Modeling of Drosophila melanogaster Cells Under the Balanced Growth Condition....Pages 445-449
Mass Transfer Considerations for Scale-Up and Scale-Down of Animal Cell Bioprocesses....Pages 451-454
Flow Characterization in Wave Bioreactors Using Computational Fluid Dynamics....Pages 455-469
Effects of Soy Peptone-Supplemented Medium on CHO-320 Cells....Pages 471-475
Very High Protein Production Levels with a New Transient and Serum-Free Expression System Based on Human CAP Cells....Pages 477-480
Front Matter....Pages 405-405
Recombinant Albumin as an Animal-Free Supplement to Enhance Cell Culture Performance....Pages 481-485
The SimcellTM High-Throughput Cell Culture System: An Automated Approach to Integrated Cell Line Selection and Process Development....Pages 487-490
Advanced In Situ Microscopy for On-Line Monitoring of Animal Cell Culture....Pages 491-496
Recombinant Antibody Yield Over 2 g/L by Transient Transfection of HEK 293 EBNA Cells in a Fed-Batch Process....Pages 497-500
Rapid Fed-Batch Process Development in SimCell™....Pages 501-505
Automation of Cell Line Development Using the OptiCHO Expression System....Pages 507-510
Modeling of the Evolution of Cellular Density and Nutrient Concentrations in a Fixed-Bed Bioreactor for Its Optimization and Its Scaling-Up....Pages 511-514
Multi-parameter Process Optimization Using the SimCell™ System....Pages 515-518
Online Measurement of pH and O2 Values During Mechanical Stimulation of Cells....Pages 519-521
Front Matter....Pages 523-523
Reducing Protein Immunogenicity by Design: Deimmunization and Tolerance Induction....Pages 525-534
Second-Generation Interferons: An Effective, Long-Lasting Hyperglycosylated IFN-alpha2....Pages 535-555
Large Scale Ex Vivo Manufacture of Blood Cells....Pages 557-571
Production of Recombinant Human Leukemia Inhibitory Factor (lif) in a Mammalian Cell Bioreactor: A First Approach....Pages 573-576
Effect of Non-Ionic Surfactant Pluronic F-68 on CHO Cell Kinetics, IFN-? Production and Glycosylation....Pages 577-580
Production and Purification of a Human Dll1(ECD)IgGFc Fusion Protein in CHOSFS Cells....Pages 581-584
Controlling Fucosylation Levels of Antibodies with Osmolality During Cell Culture in Several Host Cell Lines....Pages 585-588
A High Cell Density Approach to Fed-Batch Cell Culture for Production of Biopharmaceuticals....Pages 589-592
Approaches for Humanization of an Anti-idiotypic Murine Monoclonal Antibody....Pages 593-598
Optimized CGE-LIF-Based Glycan Analysis for High-Throughput Applications....Pages 599-603
Protein Quality Issues for Biopharmaceuticals During Solvent/Detergent Viral Inactivation Steps....Pages 605-608
Front Matter....Pages 523-523
Characterisation of Cultivation and Initial Proteome Analysis of the Novel Human Cell Line AGE1.hn....Pages 609-611
Front Matter....Pages 613-613
Avian Designer Cells AGE1.CR® as Candidates for MVA and Influenza Vaccine Production....Pages 615-631
Constitutive Augmentation of Heat Shock Response in a Producer Cell for Viral Vaccines....Pages 633-649
In Vitro Approaches for Improved Rotavirus VLP’s Quality....Pages 651-665
Development of Inactivated Polio Vaccine (IPV) Derived from Attenuated Sabin Strains....Pages 667-670
Development of a Manufacturing Process for the Production of a Canine Adenovirus Type 2 (CAV-2) Vector Using MDCK Cells....Pages 671-674
Monitoring of Host-Cell Infection and Virus-Induced Apoptosis in Influenza Vaccine Production....Pages 675-683
Accounting for the Entire Influenza Hemagglutinin during a Flu Vaccine Production Process....Pages 685-689
Lentiviral Vectors for Rapid and Efficient Recombinant CHO Cell Line Generation....Pages 691-694
Thermal and Detergent Tolerance for a Chimeric Bionanoparticle....Pages 695-698
A New Approach for Rapid Development of Spodoptera frugiperda/BEVS-Based Processes....Pages 699-703
The Role of Culture Medium Lipids and Lipid Metabolism in Retroviral Vector Production Under Serum Deprivation....Pages 705-712
Scale-Down of the Salk Inactivated Polio Vaccine Production Process....Pages 713-717
Poxvirus Production on Chicken Embryo Fibroblasts in iCELLis™ Disposable Fixed-Bed Bioreactor....Pages 719-722
Platform Technology for Viral Vaccine Production: Comparison Between Attached and Suspension Vero Cells....Pages 723-727
Front Matter....Pages 729-729
Workshop 1: New Directions in Pharmaceutical Process Development and Manufacturing: Process Analytical Technology (PAT), Quality by Design (QbD), Design Space (DS), and other FDA Initiatives....Pages 731-733
ESACT Workshop: Stem Cell Technology and Tissue Engineering New Developments and Clinical Applications....Pages 735-739
The Impact of High-Titer Feedstreams on Monoclonal Antibody Purification....Pages 741-743
Workshop on Genomics Technology....Pages 745-747
Back Matter....Pages 749-753

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The 21st ESACT meeting – ‘Cellular Solutions for Clinical Challenges’ outlined the novel capabilities of animal cell technology to move towards the clinic. The highly successful meeting was attended by a record number of researchers and engineers interested in all aspects of animal cell technology. The meeting included sessions on Stem Cells, Tissue Engineering, Advances in Bioprocessing, Biotherapeutics, Novel Vaccines and Virology and Advances in Synthetic and Molecular Biology. The meeting was supported by an array of exceptional invited speakers from around the world whose contributions complemented the emerging technologies and the challenges facing the clinical application of animal cell culture and technologies. The proceedings here include the short papers, adding to the wealth of knowledge gained from previous ESACT meetings.
Content:
Front Matter....Pages i-xliv
Front Matter....Pages 1-1
MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes....Pages 3-11
Are Clones Really Unstable?....Pages 13-18
An Optimised Transfection Platform for the Epi-CHO Transient Expression System in Serum-free Media....Pages 19-23
Using the “OMICS” Technologies as Complementary Tools to Study the Molecular Mechanisms Involved with the Adaptation of Myeloma Cell Line to Protein-Free Medium....Pages 25-29
Galectin-3 Over-Expression Enhances Survival and Recombinant Protein Expression in Mammalian Cells....Pages 31-35
Metabolomic Analysis of CHO Cultures with Different Growth Characteristics – Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells....Pages 37-41
Cell Lines in Four Weeks with the CEMAX® System....Pages 43-46
Analysis of Protein Expression via Alternate 3’ Untranslated Region (UTR) Signals Through the Use of Site Specific Recombination....Pages 47-51
A Case Study in Clone Screening: A Comprehensive Approach for a Product With High Projected Market Demand....Pages 53-56
An Improved Clone Selection Method....Pages 57-60
Dissecting the Mechanism of Action of BHRF1 for the Protection Against Apoptosis in MAb-Producing Cell Lines....Pages 61-65
Qualification of Bench and Pilot-Scale Bioreactors as Models of Commercial Systems....Pages 67-70
Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance....Pages 71-75
Implications of Vector Fragmentation and Initial Production Levels in Production Cell Line Development Using MTX....Pages 77-83
Characterizing the Expression Stability in Different Phenotypes of Recombinant NS0 Myeloma Cell Lines....Pages 85-90
A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells....Pages 91-94
Proteomic Profiling of Temperature-Shifted CHO Cells to Identify Genes that Impact on Both Cell Growth and Recombinant Protein Productivity in Suspension Culture....Pages 95-107
Stabilization of Protein Expression in Mammalian Cells Employing a Toxin/Antitoxin Based Strategy....Pages 109-113
Novel Cell Lines For Bioprocessing: Friend or Foe?....Pages 115-119
Transgene mRNA Levels and Stability are Key Factors to Enhance Transient Gene Expression in CHO DG44 Cells....Pages 121-124
Front Matter....Pages 1-1
High Cell Density Transient Gene Expression in HEK 293 EBNA Cells....Pages 125-128
Generation of High-Producing CHO Cell Lines by Piggybac Transposition....Pages 129-133
Cellular Proteins in Conditioned Medium Inhibit Polyethylenimine-Mediated Transfection of CHO Cells....Pages 135-138
A New Variant of the Affinity Matrix Method for Identification of High Producing Cells in Mammalian Cell Culture....Pages 139-142
Novel Strategies for Improving Cell Viability and Production Yield....Pages 143-145
Front Matter....Pages 147-147
Regulating in Vitro Motility of Human Mesenchymal Stem Cells with Macrophage Migration Inhibitory Factor (MIF) and a Small-Molecule MIF Antagonist....Pages 149-160
Impact of Growth Factors on the Proliferation of Ear Mesenchymal Stem Cells on Porous Microcarriers....Pages 161-176
Strategies for Pancreatic Differentiation of Pluripotent Stem Cells....Pages 177-187
Stem Cell Biology: New Applications for Studying Metabolic Diseases....Pages 189-203
Monitoring of Long-Term Cultivation and Osteogenic Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cell-Like Cultures....Pages 205-207
cGMP-Compliant Isolation, Expansion and Quality Testing of Human Bone Marrow-Derived Mesenchymal Stem Cells....Pages 209-211
Cancer Research, Molecular and Cell Biology: Look at the Nanotechnology, Based Nanoparticles for Diagnostics, and Therapy. Stem Cell for Treatment of Cancer Diseases....Pages 213-218
A Comparative Study of Suspension Cultivation Systems for the Expansion of Undifferentiated Mouse Embryonic Stem Cells....Pages 219-222
Front Matter....Pages 223-223
Human Endothelial Cell Lines with In Vivo Physiology....Pages 225-234
Quality Control and Cell Line Cross-Contamination: An Old Problem that Continues To Fester....Pages 235-239
Functional Characterisation of Human Hepatoma Cell Line HepG2 in 3D Culture....Pages 241-246
GFP-Expressing Bladder Fibroblasts for Applications in Tissue Engineering....Pages 247-250
3-Dimensional, Dynamic Cultivations of Human Umbilical Cord-Derived Cells....Pages 251-255
NIH/3T3 Feeder System to Engineer Corneal Epithelial Sheets with Enhanced Positive Progenitor Populations....Pages 257-260
NF-?B Signalling Pathway: Generation and Characterization of a Reporter Cell Line of Human Origin....Pages 261-263
Front Matter....Pages 223-223
Establishing Mammalian Production Cell Lines for Structural Biology by Site-Specific Recombination....Pages 265-268
Front Matter....Pages 269-269
Regulation of Transferrin Receptor and IGF-I Receptor Numbers at the Cell Surface Drives Growth and Productivity of Hybridoma Cells....Pages 271-283
Towards Enhancing Manufacturing Process Performance Through Multivariate Data Mining....Pages 285-294
Partial Replacement of Chemically Defined CHO Media with Plant-Derived Protein Hydrolysates....Pages 295-298
An Insight into the Physiology of Insect Cells: The Role of Energetic Metabolism on the Cell Density Effect....Pages 299-305
Design and Development of a High-Throughput Platform for Rapid Microbe Identification and Automatic Data Management....Pages 307-313
On-line Monitoring of the Live Cell Concentration in Disposable Bioreactors....Pages 315-318
The Challenge of Validating a Viable Biomass Probe for cGMP Processes....Pages 319-322
Study of the Effect of High pH and Alkali Addition in a Cultivation of Chinese Hamster Ovary Cell....Pages 323-326
Tuning of Dissolved Oxygen and pH PID Control Parameters in Large Scale Bioreactor by Lag Control....Pages 327-330
Kinetic Analysis of Recombinant BHK Roller Bottle Cultures....Pages 331-334
Manipulation of a Perfusion Process by Medium Optimization....Pages 335-340
Feed Flow Pulsation in the Separation of CHO Cells in Hydrocyclones: Effects of Pressure Drop and Pumphead Type on Separation Efficiency and Cell Viability....Pages 341-344
Development of a Biphasic Culture Process for Recombinant Protein Production in Human CAP Cells....Pages 345-349
Crossed Mixture Design and Artificial Neural Networks: An Efficient Approach to Cell Culture Medium Optimization....Pages 351-354
Improving Cell Culture Bioreactor Performance for Sensitive Cell Lines by Dynamic Membrane Aeration (DMA)....Pages 355-358
Evaluation of Disposable Bioreactor Design Compared to Reusable Stainless Steel Reactors....Pages 359-362
Utilising Scale Model Systems to Optimise Upstream Process Development....Pages 363-367
Human Platelet Lysates as a Serum Substitute in Cell Culture Media....Pages 369-373
4 g/L.day: Monoclonal Antibody Volumetric Productivity in the iCELLisTM Disposable Fixed-Bed Bioreactor....Pages 375-378
Front Matter....Pages 269-269
Optimisation of Cell Growth and Recombinant Protein Production in Small-Scale Culture Vessels by Using Optical Sensors for On-line Measurement of Dissolved Oxygen....Pages 379-382
A Protocol for Cell Detachment of Vero Cells Grown Under Fully Animal Component Free Conditions and on Cytodex 1 Microcarriers....Pages 383-386
Simulation and Optimization of Essential Amino Acids in Dynamic Mammalian Cell Culture....Pages 387-390
Biomass Sensors in iCELLis™ Fixed-Bed Reactors: Data on CHO and Vero Cells....Pages 391-394
On-line Monitoring: Animal Cell Cultivation in iCELLis™ Fixed-Bed Reactor Using Dielectric Measurements....Pages 395-399
Change of the Role for JAK/STAT3 in IL-6 Response During Adaptation of Hybridoma Cells to Serum-Free Media....Pages 401-403
Front Matter....Pages 405-405
Engineering Principles and Cell Culture Performance of Orbitally Shaken Cylindrical Bioreactors....Pages 407-412
Dynamic Ranking of Clones with Process Conditions Using a High-Throughput Micro-bioreactor Platform....Pages 413-416
Improved Cell Culture Surface for Biotechnological Research....Pages 417-420
Platform Validation of Dissolved Oxygen Ranges for Cell Culture Processes....Pages 421-424
Study of Growth Factors in Spent Medium for Better Mammalian Cell Culture....Pages 425-428
Process Improvements for Production of a Complex Glycosylated Fusion Protein which Delivers a Two-Fold Increase in Product Titre and Comparable Product Quality....Pages 429-431
Hydro-Kinetic Modelling of Animal Cell Response to Turbulent Mixing in Bioreactors....Pages 433-436
Near Infrared Spectroscopy as an In-Situ PAT Tool to Monitor Adherent VERO Cell Culture Processes....Pages 437-440
VERO Cell Metabolism in Animal Component-Free Media: Influence of Glucose and Glutamine Substitution....Pages 441-444
Metabolic Modeling of Drosophila melanogaster Cells Under the Balanced Growth Condition....Pages 445-449
Mass Transfer Considerations for Scale-Up and Scale-Down of Animal Cell Bioprocesses....Pages 451-454
Flow Characterization in Wave Bioreactors Using Computational Fluid Dynamics....Pages 455-469
Effects of Soy Peptone-Supplemented Medium on CHO-320 Cells....Pages 471-475
Very High Protein Production Levels with a New Transient and Serum-Free Expression System Based on Human CAP Cells....Pages 477-480
Front Matter....Pages 405-405
Recombinant Albumin as an Animal-Free Supplement to Enhance Cell Culture Performance....Pages 481-485
The SimcellTM High-Throughput Cell Culture System: An Automated Approach to Integrated Cell Line Selection and Process Development....Pages 487-490
Advanced In Situ Microscopy for On-Line Monitoring of Animal Cell Culture....Pages 491-496
Recombinant Antibody Yield Over 2 g/L by Transient Transfection of HEK 293 EBNA Cells in a Fed-Batch Process....Pages 497-500
Rapid Fed-Batch Process Development in SimCell™....Pages 501-505
Automation of Cell Line Development Using the OptiCHO Expression System....Pages 507-510
Modeling of the Evolution of Cellular Density and Nutrient Concentrations in a Fixed-Bed Bioreactor for Its Optimization and Its Scaling-Up....Pages 511-514
Multi-parameter Process Optimization Using the SimCell™ System....Pages 515-518
Online Measurement of pH and O2 Values During Mechanical Stimulation of Cells....Pages 519-521
Front Matter....Pages 523-523
Reducing Protein Immunogenicity by Design: Deimmunization and Tolerance Induction....Pages 525-534
Second-Generation Interferons: An Effective, Long-Lasting Hyperglycosylated IFN-alpha2....Pages 535-555
Large Scale Ex Vivo Manufacture of Blood Cells....Pages 557-571
Production of Recombinant Human Leukemia Inhibitory Factor (lif) in a Mammalian Cell Bioreactor: A First Approach....Pages 573-576
Effect of Non-Ionic Surfactant Pluronic F-68 on CHO Cell Kinetics, IFN-? Production and Glycosylation....Pages 577-580
Production and Purification of a Human Dll1(ECD)IgGFc Fusion Protein in CHOSFS Cells....Pages 581-584
Controlling Fucosylation Levels of Antibodies with Osmolality During Cell Culture in Several Host Cell Lines....Pages 585-588
A High Cell Density Approach to Fed-Batch Cell Culture for Production of Biopharmaceuticals....Pages 589-592
Approaches for Humanization of an Anti-idiotypic Murine Monoclonal Antibody....Pages 593-598
Optimized CGE-LIF-Based Glycan Analysis for High-Throughput Applications....Pages 599-603
Protein Quality Issues for Biopharmaceuticals During Solvent/Detergent Viral Inactivation Steps....Pages 605-608
Front Matter....Pages 523-523
Characterisation of Cultivation and Initial Proteome Analysis of the Novel Human Cell Line AGE1.hn....Pages 609-611
Front Matter....Pages 613-613
Avian Designer Cells AGE1.CR® as Candidates for MVA and Influenza Vaccine Production....Pages 615-631
Constitutive Augmentation of Heat Shock Response in a Producer Cell for Viral Vaccines....Pages 633-649
In Vitro Approaches for Improved Rotavirus VLP’s Quality....Pages 651-665
Development of Inactivated Polio Vaccine (IPV) Derived from Attenuated Sabin Strains....Pages 667-670
Development of a Manufacturing Process for the Production of a Canine Adenovirus Type 2 (CAV-2) Vector Using MDCK Cells....Pages 671-674
Monitoring of Host-Cell Infection and Virus-Induced Apoptosis in Influenza Vaccine Production....Pages 675-683
Accounting for the Entire Influenza Hemagglutinin during a Flu Vaccine Production Process....Pages 685-689
Lentiviral Vectors for Rapid and Efficient Recombinant CHO Cell Line Generation....Pages 691-694
Thermal and Detergent Tolerance for a Chimeric Bionanoparticle....Pages 695-698
A New Approach for Rapid Development of Spodoptera frugiperda/BEVS-Based Processes....Pages 699-703
The Role of Culture Medium Lipids and Lipid Metabolism in Retroviral Vector Production Under Serum Deprivation....Pages 705-712
Scale-Down of the Salk Inactivated Polio Vaccine Production Process....Pages 713-717
Poxvirus Production on Chicken Embryo Fibroblasts in iCELLis™ Disposable Fixed-Bed Bioreactor....Pages 719-722
Platform Technology for Viral Vaccine Production: Comparison Between Attached and Suspension Vero Cells....Pages 723-727
Front Matter....Pages 729-729
Workshop 1: New Directions in Pharmaceutical Process Development and Manufacturing: Process Analytical Technology (PAT), Quality by Design (QbD), Design Space (DS), and other FDA Initiatives....Pages 731-733
ESACT Workshop: Stem Cell Technology and Tissue Engineering New Developments and Clinical Applications....Pages 735-739
The Impact of High-Titer Feedstreams on Monoclonal Antibody Purification....Pages 741-743
Workshop on Genomics Technology....Pages 745-747
Back Matter....Pages 749-753
....