Ebook: Immunocytochemistry: A Practical Guide for Biomedical Research

Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry’s book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book’s predominant orientation is academic, focusing only on the most productive methods, with a primary emphasis on animal-based research tissues. The clear descriptions of the methods are easily followed and the specific design charts show how to plan an experiment. Included are a series of experimental cases exploring actual mistakes that demonstrate the approaches presented. Immunocytochemistry is no longer considered a specialized technique - Immunocytochemistry: A Practical Guide for Biomedical Research showcases this mainstream morphological approach in an accessible and exciting manner.

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Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry’s book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book’s predominant orientation is academic, focusing only on the most productive methods, with a primary emphasis on animal-based research tissues. The clear descriptions of the methods are easily followed and the specific design charts show how to plan an experiment. Included are a series of experimental cases exploring actual mistakes that demonstrate the approaches presented. Immunocytochemistry is no longer considered a specialized technique - Immunocytochemistry: A Practical Guide for Biomedical Research showcases this mainstream morphological approach in an accessible and exciting manner.

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Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry’s book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book’s predominant orientation is academic, focusing only on the most productive methods, with a primary emphasis on animal-based research tissues. The clear descriptions of the methods are easily followed and the specific design charts show how to plan an experiment. Included are a series of experimental cases exploring actual mistakes that demonstrate the approaches presented. Immunocytochemistry is no longer considered a specialized technique - Immunocytochemistry: A Practical Guide for Biomedical Research showcases this mainstream morphological approach in an accessible and exciting manner.

Content:
Front Matter....Pages i-xv
Introduction....Pages 1-6
Antibodies....Pages 7-16
Sample Preparation/Fixation....Pages 17-28
Tissue Sectioning....Pages 29-44
Blocking and Permeability....Pages 45-53
Labels for Antibodies....Pages 55-64
Application Methods....Pages 65-77
Controls....Pages 79-88
Method and Label Decision....Pages 89-95
Single Antibody Procedure....Pages 97-110
Multiple Antibodies Different Species....Pages 111-117
Multiple Antibodies from the Same Species....Pages 119-137
Fluorescent Microscopy and Imaging....Pages 139-149
Troubleshooting....Pages 151-174
Electron Microscopic Immunocytochemistry....Pages 175-189
Back Matter....Pages 191-223

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Immunocytochemistry is a microscopic method that uses antibodies to localize proteins in tissue sections and cell cultures. Dr. Burry’s book is the first practical guide to planning, performing, and evaluating immunocytochemical experiments designed for novice immunocytochemists. This book’s predominant orientation is academic, focusing only on the most productive methods, with a primary emphasis on animal-based research tissues. The clear descriptions of the methods are easily followed and the specific design charts show how to plan an experiment. Included are a series of experimental cases exploring actual mistakes that demonstrate the approaches presented. Immunocytochemistry is no longer considered a specialized technique - Immunocytochemistry: A Practical Guide for Biomedical Research showcases this mainstream morphological approach in an accessible and exciting manner.

Content:
Front Matter....Pages i-xv
Introduction....Pages 1-6
Antibodies....Pages 7-16
Sample Preparation/Fixation....Pages 17-28
Tissue Sectioning....Pages 29-44
Blocking and Permeability....Pages 45-53
Labels for Antibodies....Pages 55-64
Application Methods....Pages 65-77
Controls....Pages 79-88
Method and Label Decision....Pages 89-95
Single Antibody Procedure....Pages 97-110
Multiple Antibodies Different Species....Pages 111-117
Multiple Antibodies from the Same Species....Pages 119-137
Fluorescent Microscopy and Imaging....Pages 139-149
Troubleshooting....Pages 151-174
Electron Microscopic Immunocytochemistry....Pages 175-189
Back Matter....Pages 191-223
....