Ebook: Chromosomal Alterations: Methods, Results and Importance in Human Health
Author: Heidemarie Neitzel Marc Trimborn (auth.) Professor Dr. Günter Obe Professor Dr. Vijayalaxmi (eds.)
- Tags: Cell Biology, Proteomics, Animal Genetics and Genomics, Cancer Research
- Year: 2007
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
Cytogenetics is a very important research tool in basic and applied research. The uses of cytogenetics in human-population monitoring, in biological dosimetry in radiation accidents and in astronauts and as a predictive measure of cancer are topics discussed in this book. The book will help the reader to better understand cytogenetics and the intricacies of the methodology. The different methods of fluorescence in situ hybridization are discussed and the results achieved are presented. The book provides a comprehensive review of basic and applied aspects of cytogenetics and therefore will be of interest to all who are interested in chromosomes and their alterations by different types of mutagens, including chemical mutagens and ionizing and nonionizing radiation, with special reference to electromagnetic fields.
Cytogenetics is a very important research tool in basic and applied research. The uses of cytogenetics in human-population monitoring, in biological dosimetry in radiation accidents and in astronauts and as a predictive measure of cancer are topics discussed in this book. The book will help the reader to better understand cytogenetics and the intricacies of the methodology. The different methods of fluorescence in situ hybridization are discussed and the results achieved are presented. The book provides a comprehensive review of basic and applied aspects of cytogenetics and therefore will be of interest to all who are interested in chromosomes and their alterations by different types of mutagens, including chemical mutagens and ionizing and nonionizing radiation, with special reference to electromagnetic fields.
Cytogenetics is a very important research tool in basic and applied research. The uses of cytogenetics in human-population monitoring, in biological dosimetry in radiation accidents and in astronauts and as a predictive measure of cancer are topics discussed in this book. The book will help the reader to better understand cytogenetics and the intricacies of the methodology. The different methods of fluorescence in situ hybridization are discussed and the results achieved are presented. The book provides a comprehensive review of basic and applied aspects of cytogenetics and therefore will be of interest to all who are interested in chromosomes and their alterations by different types of mutagens, including chemical mutagens and ionizing and nonionizing radiation, with special reference to electromagnetic fields.
Content:
Front Matter....Pages i-xxiv
Human Chromosomes: Structural and Functional Aspects....Pages 1-20
DNA Lesions Induced by Ionizing Radiation....Pages 21-38
Effects of Ultraviolet Radiation on DNA....Pages 39-53
Double Strand Break Repair Mechanisms in Mammalian Cells....Pages 55-65
Backup Pathways of Nonhomologous End Joining May Have a Dominant Role in the Formation of Chromosome Aberrations....Pages 67-85
Targeted and Non-Targeted Induction of Chromosomal Rearrangements After Exposure to Ionizing Radiation....Pages 87-98
DNA Methylation Damage: Formation, Repair and Biological Consequences....Pages 99-121
Adducts, Sister-Chromatid Exchanges and Mutations Following Benzo[a]pyrene Exposure: a Review of Quantitative Data Followed by Some Considerations Regarding Risk....Pages 123-143
Analysis of DNA Double-Strand Breaks by Means of ?-H2AX Foci....Pages 145-160
Comet Assay....Pages 161-176
Origin of Chromosome Aberrations: Mechanisms....Pages 177-199
Chromatin Structure and the Formation of Chromosomal Alterations....Pages 201-209
Role of Chromatin Structure and Activity in the Induction of Chromosomal Aberrations....Pages 211-224
A Biomedical Perspective of Telomere Structure and Function....Pages 225-240
Cytokinesis-Block Micronucleus Assay: a Comprehensive “Cytome” Approach for Measuring Chromosomal Instability, Mitotic Dysfunction and Cell Death Simultaneously in One Assay....Pages 241-255
In Vivo Rodent Micronucleus Assay....Pages 257-270
Sister-Chromatid Exchanges....Pages 271-283
Fluorescence in Situ Hybridisation....Pages 285-299
The CAB and S&S Systems, and an Approach Towards the Classification of Complex Chromosome Exchanges....Pages 301-314
Aberration Patterns and Cell Cycle Progression Following Exposure of Lymphocytes to the Alkylating Agent Trenimon....Pages 315-324
Cytogenetic Analysis and Occupational Health....Pages 325-340
Biological Dosimetry....Pages 341-350
Statistical Methods for Biological Dosimetry....Pages 351-370
Retrospective Biological Dosimetry by FISH....Pages 371-380
Chromosomal Aberrations in Astronauts....Pages 381-396
Internet Information System and Literature Database on Biomedical Activities of Electromagnetic Fields: Cancer and Cytogenetic Effects....Pages 397-409
Interpreting Studies on Magnetic Fields and the Risk of Childhood Leukaemia: an Ongoing Challenge....Pages 411-426
Cytogenetic and Carcinogenic Effects of Exposure to Radiofrequency Radiation....Pages 427-469
Radiofrequency Radiation and Replication Studies....Pages 471-478
Chromosome Analysis in Cancer Patients: Applications and Limitations....Pages 479-493
Chromosomal Aberration in Peripheral Blood Lymphocytes of Healthy Subjects and Risk of Cancer....Pages 495-504
Back Matter....Pages 505-515
Cytogenetics is a very important research tool in basic and applied research. The uses of cytogenetics in human-population monitoring, in biological dosimetry in radiation accidents and in astronauts and as a predictive measure of cancer are topics discussed in this book. The book will help the reader to better understand cytogenetics and the intricacies of the methodology. The different methods of fluorescence in situ hybridization are discussed and the results achieved are presented. The book provides a comprehensive review of basic and applied aspects of cytogenetics and therefore will be of interest to all who are interested in chromosomes and their alterations by different types of mutagens, including chemical mutagens and ionizing and nonionizing radiation, with special reference to electromagnetic fields.
Content:
Front Matter....Pages i-xxiv
Human Chromosomes: Structural and Functional Aspects....Pages 1-20
DNA Lesions Induced by Ionizing Radiation....Pages 21-38
Effects of Ultraviolet Radiation on DNA....Pages 39-53
Double Strand Break Repair Mechanisms in Mammalian Cells....Pages 55-65
Backup Pathways of Nonhomologous End Joining May Have a Dominant Role in the Formation of Chromosome Aberrations....Pages 67-85
Targeted and Non-Targeted Induction of Chromosomal Rearrangements After Exposure to Ionizing Radiation....Pages 87-98
DNA Methylation Damage: Formation, Repair and Biological Consequences....Pages 99-121
Adducts, Sister-Chromatid Exchanges and Mutations Following Benzo[a]pyrene Exposure: a Review of Quantitative Data Followed by Some Considerations Regarding Risk....Pages 123-143
Analysis of DNA Double-Strand Breaks by Means of ?-H2AX Foci....Pages 145-160
Comet Assay....Pages 161-176
Origin of Chromosome Aberrations: Mechanisms....Pages 177-199
Chromatin Structure and the Formation of Chromosomal Alterations....Pages 201-209
Role of Chromatin Structure and Activity in the Induction of Chromosomal Aberrations....Pages 211-224
A Biomedical Perspective of Telomere Structure and Function....Pages 225-240
Cytokinesis-Block Micronucleus Assay: a Comprehensive “Cytome” Approach for Measuring Chromosomal Instability, Mitotic Dysfunction and Cell Death Simultaneously in One Assay....Pages 241-255
In Vivo Rodent Micronucleus Assay....Pages 257-270
Sister-Chromatid Exchanges....Pages 271-283
Fluorescence in Situ Hybridisation....Pages 285-299
The CAB and S&S Systems, and an Approach Towards the Classification of Complex Chromosome Exchanges....Pages 301-314
Aberration Patterns and Cell Cycle Progression Following Exposure of Lymphocytes to the Alkylating Agent Trenimon....Pages 315-324
Cytogenetic Analysis and Occupational Health....Pages 325-340
Biological Dosimetry....Pages 341-350
Statistical Methods for Biological Dosimetry....Pages 351-370
Retrospective Biological Dosimetry by FISH....Pages 371-380
Chromosomal Aberrations in Astronauts....Pages 381-396
Internet Information System and Literature Database on Biomedical Activities of Electromagnetic Fields: Cancer and Cytogenetic Effects....Pages 397-409
Interpreting Studies on Magnetic Fields and the Risk of Childhood Leukaemia: an Ongoing Challenge....Pages 411-426
Cytogenetic and Carcinogenic Effects of Exposure to Radiofrequency Radiation....Pages 427-469
Radiofrequency Radiation and Replication Studies....Pages 471-478
Chromosome Analysis in Cancer Patients: Applications and Limitations....Pages 479-493
Chromosomal Aberration in Peripheral Blood Lymphocytes of Healthy Subjects and Risk of Cancer....Pages 495-504
Back Matter....Pages 505-515
....