Ebook: Imaging Cellular and Molecular Biological Functions
- Tags: Cell Biology, Biochemistry general, Biological Microscopy, Biological Techniques, Biotechnology
- Series: Principles and Practice
- Year: 2007
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
‘Imaging cellular and molecular biological function’ provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.
‘Imaging cellular and molecular biological function’ provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.
‘Imaging cellular and molecular biological function’ provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.
Content:
Front Matter....Pages i-xxi
Front Matter....Pages 2-2
Entering the Portal: Understanding the Digital Image Recorded Through a Microscope....Pages 3-43
Quantitative Biological Image Analysis....Pages 45-70
The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics....Pages 71-92
Design and Function of a Light-Microscopy Facility....Pages 93-113
Front Matter....Pages 116-116
Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls....Pages 117-155
Quantitative FRET Microscopy of Live Cells....Pages 157-181
Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells....Pages 183-233
Single Fluorescent Molecule Tracking in Live Cells....Pages 235-263
From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins....Pages 265-285
Front Matter....Pages 288-288
Towards Imaging the Dynamics of Protein Signalling....Pages 289-312
New Technologies for Imaging and Analysis of Individual Microbial Cells....Pages 313-343
Imaging Parasites in Vivo....Pages 345-364
Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells....Pages 365-384
High-Throughput/High-Content Automated Image Acquisition and Analysis....Pages 385-405
Cognition Network Technology – A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents....Pages 407-422
High-Content Phenotypic Cell-Based Assays....Pages 423-442
Back Matter....Pages 443-449
‘Imaging cellular and molecular biological function’ provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.
Content:
Front Matter....Pages i-xxi
Front Matter....Pages 2-2
Entering the Portal: Understanding the Digital Image Recorded Through a Microscope....Pages 3-43
Quantitative Biological Image Analysis....Pages 45-70
The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics....Pages 71-92
Design and Function of a Light-Microscopy Facility....Pages 93-113
Front Matter....Pages 116-116
Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls....Pages 117-155
Quantitative FRET Microscopy of Live Cells....Pages 157-181
Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells....Pages 183-233
Single Fluorescent Molecule Tracking in Live Cells....Pages 235-263
From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins....Pages 265-285
Front Matter....Pages 288-288
Towards Imaging the Dynamics of Protein Signalling....Pages 289-312
New Technologies for Imaging and Analysis of Individual Microbial Cells....Pages 313-343
Imaging Parasites in Vivo....Pages 345-364
Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells....Pages 365-384
High-Throughput/High-Content Automated Image Acquisition and Analysis....Pages 385-405
Cognition Network Technology – A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents....Pages 407-422
High-Content Phenotypic Cell-Based Assays....Pages 423-442
Back Matter....Pages 443-449
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