Ebook: Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis
- Tags: Cell Biology
- Series: Methods in Molecular Biology™ 348
- Year: 2006
- Publisher: Humana Press
- Edition: 1
- Language: English
- pdf
Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones.
Content:
Front Matter....Pages i-xi
Front Matter....Pages 197-197
Front Matter....Pages 3-31
Front Matter....Pages 35-42
Back Matter....Pages 43-58
....Pages 59-77
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