Ebook: Mammalian and Avian Transgenesis — New Approaches
- Tags: Animal Genetics and Genomics, Cell Biology, Developmental Biology, Molecular Medicine
- Series: Principles and Practice
- Year: 2006
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds.
The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds.
The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds.
The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Content:
Front Matter....Pages I-XX
Generation of Transgenic Animals Using Lentiviral Vectors....Pages 1-22
Intracytoplasmic Sperm Injection (ICSI) in the Mouse....Pages 23-40
Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation....Pages 41-68
Cloning The Laboratory Mouse by Nuclear Transfer....Pages 69-96
Large Insert Transgenesis....Pages 97-110
Regional and Temporal Control of Genetic Manipulation in the Mouse....Pages 111-130
High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes....Pages 131-172
Nuclear Transfer in the Cow....Pages 173-193
Production of Transgenic Pigs by DNA Microinjection....Pages 195-220
Production of Transgenic Birds Using Lentiviral Vectors....Pages 221-230
Ancillary Techniques....Pages 231-276
Back Matter....Pages 277-281
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds.
The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Content:
Front Matter....Pages I-XX
Generation of Transgenic Animals Using Lentiviral Vectors....Pages 1-22
Intracytoplasmic Sperm Injection (ICSI) in the Mouse....Pages 23-40
Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation....Pages 41-68
Cloning The Laboratory Mouse by Nuclear Transfer....Pages 69-96
Large Insert Transgenesis....Pages 97-110
Regional and Temporal Control of Genetic Manipulation in the Mouse....Pages 111-130
High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes....Pages 131-172
Nuclear Transfer in the Cow....Pages 173-193
Production of Transgenic Pigs by DNA Microinjection....Pages 195-220
Production of Transgenic Birds Using Lentiviral Vectors....Pages 221-230
Ancillary Techniques....Pages 231-276
Back Matter....Pages 277-281
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