Ebook: Basic Methods for the Biochemical Lab

Basic Methods for the Biochemical Lab elucidates proven lab procedures and practical hints for research in analytical and preparative biochemistry, as well as summarizing key data in numerous tables. To further emphasize the practical aspects and to minimize the text, theoretical introductions into the methods are mostly omitted.

The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates), electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis), chromatographic protocols (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC), immunochemical protocols (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA), centrifugation (differential and density gradient centrifugation for cells and cellular fractions), radioactivity (labeling and counting), buffers (buffer properties and compositions).

In additional chapters, tables compile a broad array of useful information, e.g. SI units conversion factor, detergent, protein and nucleotide data, and the basic principles of statistics and enzyme and receptor kinetics are reviewed.

This first English-language edition of a successful German-language manual will be a valuable resource for students and experts in biochemistry, biotechnology and biomedical laboratories.

Basic Methods for the Biochemical Lab presents proven lab procedures and practical hints for research in analytical and preparative biochemistry, and offers convenient key data in numerous tables.

The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates); electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis); chromatographic protocols (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC); immunochemical protocols (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA); centrifugation (differential and density gradient centrifugation for cells and cellular fractions); radioactivity (labeling and counting); and buffers (buffer properties and compositions).

In additional chapters, tables provide speedy access to a broad array of useful information, including SI units conversion factors; detergent, protein and nucleotide data; and the basic principles of statistics and enzyme and receptor kinetics are reviewed.

This first English-language edition of

Basic Methods for the Biochemical Lab presents proven lab procedures and practical hints for research in analytical and preparative biochemistry, and offers convenient key data in numerous tables.

The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates); electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis); chromatographic protocols (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC); immunochemical protocols (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA); centrifugation (differential and density gradient centrifugation for cells and cellular fractions); radioactivity (labeling and counting); and buffers (buffer properties and compositions).

In additional chapters, tables provide speedy access to a broad array of useful information, including SI units conversion factors; detergent, protein and nucleotide data; and the basic principles of statistics and enzyme and receptor kinetics are reviewed.

This first English-language edition of
Content:
Front Matter....Pages I-XVIII
Quantitative Methods....Pages 1-21
Electrophoresis....Pages 23-82
Chromatography....Pages 83-128
Immunochemical Protocols....Pages 129-160
Centrifugation....Pages 161-180
Radioactive Labeling....Pages 181-189
Buffers....Pages 191-207
Tables....Pages 209-232
Statistics and Data Analysis....Pages 233-246
Back Matter....Pages 247-251

Basic Methods for the Biochemical Lab presents proven lab procedures and practical hints for research in analytical and preparative biochemistry, and offers convenient key data in numerous tables.

The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates); electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis); chromatographic protocols (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC); immunochemical protocols (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA); centrifugation (differential and density gradient centrifugation for cells and cellular fractions); radioactivity (labeling and counting); and buffers (buffer properties and compositions).

In additional chapters, tables provide speedy access to a broad array of useful information, including SI units conversion factors; detergent, protein and nucleotide data; and the basic principles of statistics and enzyme and receptor kinetics are reviewed.

This first English-language edition of
Content:
Front Matter....Pages I-XVIII
Quantitative Methods....Pages 1-21
Electrophoresis....Pages 23-82
Chromatography....Pages 83-128
Immunochemical Protocols....Pages 129-160
Centrifugation....Pages 161-180
Radioactive Labeling....Pages 181-189
Buffers....Pages 191-207
Tables....Pages 209-232
Statistics and Data Analysis....Pages 233-246
Back Matter....Pages 247-251
....