Ebook: Protocol for Somatic Embryogenesis in Woody Plants

Earlier, we edited a well received series on "Somatic embryogenesis in woody trees", volumes 1 to 6. These volumes provided readers extensive reviews on somatic embryogenesis of important angiosperm and gymnosperm tree species; an excellent source of information for newcomers or those already engaged in research. However, these book volumes did not cover stepwise "detailed protocols" for inducing somatic embryogenesis. This book contains 46 chapters, divided into 4 sections: A) 12 chapters on conifers, B) 14 chapters on fruits, C) 14 chapters on angiosperms, and D) 6 chapters on histology, bioencapsulation, protoplasts, cryopreservation, double staining and thin cell layer sectioning. The book provides stepwise protocols for somatic embryogenesis of a range of selected woody plants in order to assist researchers to initiate somatic embryogenic cultures without too much alterations in protocols. Each chapter provides information on initiation and maintenance of embryogenic cultures; somatic embryo development, maturation, and germination; acclimitization and field transfer of somatic seedlings. Some chapteres include applications of somatic embryogenesis cultures, e.g. protoplasts, encapsulation, cryopreservation, genetic transformation, genetic fidelity with molecular markers, and bioreactor.

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Earlier, we edited a well received series on "Somatic embryogenesis in woody trees", volumes 1 to 6. These volumes provided readers extensive reviews on somatic embryogenesis of important angiosperm and gymnosperm tree species; an excellent source of information for newcomers or those already engaged in research. However, these book volumes did not cover stepwise "detailed protocols" for inducing somatic embryogenesis. This book contains 46 chapters, divided into 4 sections: A) 12 chapters on conifers, B) 14 chapters on fruits, C) 14 chapters on angiosperms, and D) 6 chapters on histology, bioencapsulation, protoplasts, cryopreservation, double staining and thin cell layer sectioning. The book provides stepwise protocols for somatic embryogenesis of a range of selected woody plants in order to assist researchers to initiate somatic embryogenic cultures without too much alterations in protocols. Each chapter provides information on initiation and maintenance of embryogenic cultures; somatic embryo development, maturation, and germination; acclimitization and field transfer of somatic seedlings. Some chapteres include applications of somatic embryogenesis cultures, e.g. protoplasts, encapsulation, cryopreservation, genetic transformation, genetic fidelity with molecular markers, and bioreactor.

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Earlier, we edited a well received series on "Somatic embryogenesis in woody trees", volumes 1 to 6. These volumes provided readers extensive reviews on somatic embryogenesis of important angiosperm and gymnosperm tree species; an excellent source of information for newcomers or those already engaged in research. However, these book volumes did not cover stepwise "detailed protocols" for inducing somatic embryogenesis. This book contains 46 chapters, divided into 4 sections: A) 12 chapters on conifers, B) 14 chapters on fruits, C) 14 chapters on angiosperms, and D) 6 chapters on histology, bioencapsulation, protoplasts, cryopreservation, double staining and thin cell layer sectioning. The book provides stepwise protocols for somatic embryogenesis of a range of selected woody plants in order to assist researchers to initiate somatic embryogenic cultures without too much alterations in protocols. Each chapter provides information on initiation and maintenance of embryogenic cultures; somatic embryo development, maturation, and germination; acclimitization and field transfer of somatic seedlings. Some chapteres include applications of somatic embryogenesis cultures, e.g. protoplasts, encapsulation, cryopreservation, genetic transformation, genetic fidelity with molecular markers, and bioreactor.
Content:
Front Matter....Pages i-x
Slash Pine (Pinus elliottii Engelm.)....Pages 1-10
Douglas - Fir (Pseudotsuga menziesii)....Pages 11-24
Omorika Spruce (Picea omorika)....Pages 25-34
Protocol of Somatic Embryogenesis: Black Spruce (Picea mariana (Mill.) B.S.P.)....Pages 35-45
Sitka Spruce (Picea sitchensis)....Pages 47-58
Protocol of Somatic Embryogenesis of Pinus nigra Arn.....Pages 59-68
Loblolly Pine (Pinus taeda)....Pages 69-80
Somatic Embryogenesis in Maritime Pine (Pinus pinaster Ait.)....Pages 81-93
Somatic Embryogenesis in Norway Spruce....Pages 95-106
Cashew (Anacardium occidentale L.)....Pages 107-119
Somatic Embryogenesis Protocol: Coffee (Coffea arabica L. and C. canephora P.)....Pages 121-139
Protocols for Somatic Embryogenesis and Plantlet Formation from Three Explants in Tea (Camellia sinensis (l.) o. kuntze)....Pages 141-155
Protocol of Somatic Embryogenesis from Citrus spp. Anther Culture....Pages 157-165
Mango (Mangifera indica L.)....Pages 167-179
Somatic Embryogenesis Injackfruit (Artocarpus heterophyllus Lam.)....Pages 181-190
Somatic Embryogenesis in Indian Olive (Elaeocarpus robustus L)....Pages 191-207
Rescue of Endangered Palms by in vitro Methods: The Case of ‘Bottle Palm’....Pages 209-227
Somatic Embryogenesis in American Grapes (Vitis x labruscana L.H. Bailey)....Pages 229-246
Pistachio (Pistacia vera L.)....Pages 247-256
Date Palm Phoenix dactylifera L.....Pages 257-266
Olive (Olea europaea L.)....Pages 267-274
Protocol of Somatic Embryogenesis: Dalbergia sissoo Roxb. (Sissoo)....Pages 275-287
Protocol of Somatic Embryogenesis: Pedunculate Oak (Quercus robur L.) and Sessile Oak (Quercus petraea /Matt./ Liebl.)....Pages 289-300
Protocol of Somatic Embryogenesis: Tamarillo (Cyphomandra betacea (Cav.) Sendtn.)....Pages 301-308
Protocol of Somatic Embryogenesis: European Chestnut (Castanea sativa Mill.)....Pages 309-319
Protocol of Somatic Embryogenesis in Acacia arabica (Lamk.) Willd.....Pages 321-343
Protocol for Hazelnut Somatic Embryogenesis....Pages 345-360
Protocol of Somatic Embryogenesis: Ocotea catharinensis Mez. (Lauraceae)....Pages 361-368
Cork Oak, Quercus suber L.....Pages 369-378
Sawara Cypress Chamaecyparis pisifera Sieb. et Zucc.....Pages 379-389
Protocol of Somatic Embryogenesis: Holm Oak (Quercus ilex L.)....Pages 391-399
Protocols for Somatic Embryogenesis of Hybrid Firs....Pages 401-411
Somatic Embryogenesis in Sandalwood....Pages 413-426
Histological Techniques....Pages 427-443
Bioencapsulation of Somatic Embryos in Woody Plants....Pages 445-457
Protoplast Isolation and Culture of Woody Plants....Pages 459-468
Cryopreservation of Embryonal Cells....Pages 469-482
Double Staining Technology for Distinguishing Embryogenic Cultures....Pages 483-496
Thin Cell Layer Sectioning for Inducing Somatic Embryogenesis in Woody Plants....Pages 497-504
Back Matter....Pages 505-515
....Pages 517-537