Ebook: Dissecting the Molecular Anatomy of Tissue
- Tags: Cell Biology, Immunology, Biochemistry general, Anatomy, Cancer Research
- Series: Principles and Practice
- Year: 2005
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases. Special emphasis is placed on human cancer and the future directions of the field. Methods of handling clinical tissue samples, including the impact of handling on subsequent molecular analysis, are also discussed. In addition, detailed protocols for molecular analysis of DNA, RNA and protein, with special emphasis on molecular analysis of highly complex human tissue samples containing mixtures of cell populations, are provided.
The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases. Special emphasis is placed on human cancer and the future directions of the field. Methods of handling clinical tissue samples, including the impact of handling on subsequent molecular analysis, are also discussed. In addition, detailed protocols for molecular analysis of DNA, RNA and protein, with special emphasis on molecular analysis of highly complex human tissue samples containing mixtures of cell populations, are provided.
The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases. Special emphasis is placed on human cancer and the future directions of the field. Methods of handling clinical tissue samples, including the impact of handling on subsequent molecular analysis, are also discussed. In addition, detailed protocols for molecular analysis of DNA, RNA and protein, with special emphasis on molecular analysis of highly complex human tissue samples containing mixtures of cell populations, are provided.
Content:
Front Matter....Pages I-XVII
Molecular Pathology Laboratory of the Future....Pages 3-15
Clinical Utilities of Microdissected Samples....Pages 17-26
Tissue Processing....Pages 27-42
Sample Management and Tracking....Pages 43-53
Tissue Microarrays....Pages 57-66
Methods of Microdissection and New Technologies....Pages 69-100
Standard Diagnostic Techniques....Pages 101-110
DNA Applications....Pages 111-123
mRNA Applications....Pages 125-144
Proteomic Applications....Pages 145-162
Protocols for Tissue Handling and Molecular Analysis....Pages 163-220
Back Matter....Pages 221-224
The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases. Special emphasis is placed on human cancer and the future directions of the field. Methods of handling clinical tissue samples, including the impact of handling on subsequent molecular analysis, are also discussed. In addition, detailed protocols for molecular analysis of DNA, RNA and protein, with special emphasis on molecular analysis of highly complex human tissue samples containing mixtures of cell populations, are provided.
Content:
Front Matter....Pages I-XVII
Molecular Pathology Laboratory of the Future....Pages 3-15
Clinical Utilities of Microdissected Samples....Pages 17-26
Tissue Processing....Pages 27-42
Sample Management and Tracking....Pages 43-53
Tissue Microarrays....Pages 57-66
Methods of Microdissection and New Technologies....Pages 69-100
Standard Diagnostic Techniques....Pages 101-110
DNA Applications....Pages 111-123
mRNA Applications....Pages 125-144
Proteomic Applications....Pages 145-162
Protocols for Tissue Handling and Molecular Analysis....Pages 163-220
Back Matter....Pages 221-224
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