Ebook: Homing Endonucleases and Inteins
- Tags: Biochemistry general, Cell Biology, Biochemical Engineering, Genetic Engineering
- Series: Nucleic Acids and Molecular Biology 16
- Year: 2005
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.
This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.
This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.
Content:
Front Matter....Pages I-XIX
Back to Basics: Structure, Function, Evolution and Application of Homing Endonucleases and Inteins....Pages 1-10
Homing Endonucleases and the Yeast Mitochondrial ? Locus — A Historical Perspective....Pages 11-31
The LAGLIDADG Homing Endonuclease Family....Pages 33-47
HNH Endonucleases....Pages 49-65
GIY-YIG Homing Endonucleases — Beads on a String....Pages 67-83
His-Cys Box Homing Endonucleases....Pages 85-102
Group I Introns and Their Maturases: Uninvited, but Welcome Guests....Pages 103-119
Group II Intron Homing Endonucleases: Ribonucleoprotein Complexes with Programmable Target Specificity....Pages 121-145
Free-Standing Homing Endonucleases of T-even Phage: Freeloaders or Functionaries?....Pages 147-160
Function and Evolution of HO and VDE Endonucleases in Fungi....Pages 161-175
Engineering Homing Endonucleases for Genomic Applications....Pages 177-192
Inteins — A Historical Perspective....Pages 193-210
Origin and Evolution of Inteins and Other Hint Domains....Pages 211-231
Biochemical Mechanisms of Intein-Mediated Protein Splicing....Pages 233-255
The Structure and Function of Intein-Associated Homing Endonucleases....Pages 257-271
Harnessing Inteins for Protein Purification and Characterization....Pages 273-292
Production of Cyclic Proteins and Peptides....Pages 293-305
Inteins for Split-Protein Reconstitutions and Their Applications....Pages 307-323
Intein Reporter and Selection Systems....Pages 325-344
Industrial Applications of Intein Technology....Pages 345-364
Back Matter....Pages 365-377
This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.
Content:
Front Matter....Pages I-XIX
Back to Basics: Structure, Function, Evolution and Application of Homing Endonucleases and Inteins....Pages 1-10
Homing Endonucleases and the Yeast Mitochondrial ? Locus — A Historical Perspective....Pages 11-31
The LAGLIDADG Homing Endonuclease Family....Pages 33-47
HNH Endonucleases....Pages 49-65
GIY-YIG Homing Endonucleases — Beads on a String....Pages 67-83
His-Cys Box Homing Endonucleases....Pages 85-102
Group I Introns and Their Maturases: Uninvited, but Welcome Guests....Pages 103-119
Group II Intron Homing Endonucleases: Ribonucleoprotein Complexes with Programmable Target Specificity....Pages 121-145
Free-Standing Homing Endonucleases of T-even Phage: Freeloaders or Functionaries?....Pages 147-160
Function and Evolution of HO and VDE Endonucleases in Fungi....Pages 161-175
Engineering Homing Endonucleases for Genomic Applications....Pages 177-192
Inteins — A Historical Perspective....Pages 193-210
Origin and Evolution of Inteins and Other Hint Domains....Pages 211-231
Biochemical Mechanisms of Intein-Mediated Protein Splicing....Pages 233-255
The Structure and Function of Intein-Associated Homing Endonucleases....Pages 257-271
Harnessing Inteins for Protein Purification and Characterization....Pages 273-292
Production of Cyclic Proteins and Peptides....Pages 293-305
Inteins for Split-Protein Reconstitutions and Their Applications....Pages 307-323
Intein Reporter and Selection Systems....Pages 325-344
Industrial Applications of Intein Technology....Pages 345-364
Back Matter....Pages 365-377
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