Ebook: Restriction Endonucleases
Author: R. J. Roberts M. Belfort T. Bestor A. S. Bhagwat (auth.) Professor Dr. Alfred M. Pingoud (eds.)
- Tags: Biochemistry general, Biotechnology, Cell Biology, Microbiology
- Series: Nucleic Acids and Molecular Biology 14
- Year: 2004
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
The present book deals with all aspects of restriction endonucleases including nomenclature, diversity, evolution, genetics, structure and function, mechanism of target site location and DNA recognition, enzymology, protein design, and provides a description of the history of the discovery of and the research on restriction enzymes.
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
The present book deals with all aspects of restriction endonucleases including nomenclature, diversity, evolution, genetics, structure and function, mechanism of target site location and DNA recognition, enzymology, protein design, and provides a description of the history of the discovery of and the research on restriction enzymes.
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
The present book deals with all aspects of restriction endonucleases including nomenclature, diversity, evolution, genetics, structure and function, mechanism of target site location and DNA recognition, enzymology, protein design, and provides a description of the history of the discovery of and the research on restriction enzymes.
Content:
Front Matter....Pages I-XXVI
A Nomenclature for Restriction Enzymes, DNA Methyltransferases, Homing Endonucleases, and Their Genes....Pages 1-18
Restriction-Modification Systems as Minimal Forms of Life....Pages 19-62
Molecular Phylogenetics of Restriction Endonucleases....Pages 63-93
Sliding or Hopping? How Restriction Enzymes Find Their Way on DNA....Pages 95-110
The Type I and III Restriction Endonucleases: Structural Elements in Molecular Motors that Process DNA....Pages 111-135
The Integration of Recognition and Cleavage: X-Ray Structures of Pre-Transition State Complex, Post-Reactive Complex, and the DNA-Free Endonuclease....Pages 137-177
Structure and Function of EcoRV Endonuclease....Pages 179-214
Two of A Kind: BamHI and BglII....Pages 215-236
Structure and Function of the Tetrameric Restriction Enzymes....Pages 237-259
Structure and Function of Type IIE Restriction Endonucleases — or: From a Plasmid That Restricts Phage Replication to A New Molecular DNA Recognition Mechanism....Pages 261-295
Analysis of Type II Restriction Endonucleases that Interact with Two Recognition Sites....Pages 297-317
The Role of Water in the EcoRI-DNA Binding....Pages 319-337
Role of Metal Ions in Promoting DNA Binding and Cleavage by Restriction Endonucleases....Pages 339-360
Restriction Endonucleases: Structure of the Conserved Catalytic Core and the Role of Metal Ions in DNA Cleavage....Pages 361-392
Protein Engineering of Restriction Enzymes....Pages 393-411
Engineering and Applications of Chimeric Nucleases....Pages 413-434
Back Matter....Pages 435-443
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
The present book deals with all aspects of restriction endonucleases including nomenclature, diversity, evolution, genetics, structure and function, mechanism of target site location and DNA recognition, enzymology, protein design, and provides a description of the history of the discovery of and the research on restriction enzymes.
Content:
Front Matter....Pages I-XXVI
A Nomenclature for Restriction Enzymes, DNA Methyltransferases, Homing Endonucleases, and Their Genes....Pages 1-18
Restriction-Modification Systems as Minimal Forms of Life....Pages 19-62
Molecular Phylogenetics of Restriction Endonucleases....Pages 63-93
Sliding or Hopping? How Restriction Enzymes Find Their Way on DNA....Pages 95-110
The Type I and III Restriction Endonucleases: Structural Elements in Molecular Motors that Process DNA....Pages 111-135
The Integration of Recognition and Cleavage: X-Ray Structures of Pre-Transition State Complex, Post-Reactive Complex, and the DNA-Free Endonuclease....Pages 137-177
Structure and Function of EcoRV Endonuclease....Pages 179-214
Two of A Kind: BamHI and BglII....Pages 215-236
Structure and Function of the Tetrameric Restriction Enzymes....Pages 237-259
Structure and Function of Type IIE Restriction Endonucleases — or: From a Plasmid That Restricts Phage Replication to A New Molecular DNA Recognition Mechanism....Pages 261-295
Analysis of Type II Restriction Endonucleases that Interact with Two Recognition Sites....Pages 297-317
The Role of Water in the EcoRI-DNA Binding....Pages 319-337
Role of Metal Ions in Promoting DNA Binding and Cleavage by Restriction Endonucleases....Pages 339-360
Restriction Endonucleases: Structure of the Conserved Catalytic Core and the Role of Metal Ions in DNA Cleavage....Pages 361-392
Protein Engineering of Restriction Enzymes....Pages 393-411
Engineering and Applications of Chimeric Nucleases....Pages 413-434
Back Matter....Pages 435-443
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