Ebook: Methods in Proteome and Protein Analysis
- Tags: Proteomics, Biochemistry general, Bioinformatics
- Series: Principles and Practice
- Year: 2004
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, "Methods in Proteome and Protein Analysis" presents the most interesting papers from the 14th MPSA meeting.
Selected contributions discuss the latest techniques and approaches for analysing protein and proteome structures. Major topics include:
protein and peptide sample preparation and separationnew reagent for protein sequence analysis
mass spectrometry in protein research
analysis of posttranslational modification
protein-protein interaction using MALDI-MS
manipulation of genome or functional compositon trap
structure-function correlation study using optical biosensors of microcolorimetrical techniques
structural proteomics as NMR or fluorescence polarization study
the classification and prediction of structure or functional sites
in silico analysis of proteins and proteomes
increasing throughput and data quality for proteomics
Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, "Methods in Proteome and Protein Analysis" presents the most interesting papers from the 14th MPSA meeting.
Selected contributions discuss the latest techniques and approaches for analysing protein and proteome structures. Major topics include:
protein and peptide sample preparation and separationnew reagent for protein sequence analysis
mass spectrometry in protein research
analysis of posttranslational modification
protein-protein interaction using MALDI-MS
manipulation of genome or functional compositon trap
structure-function correlation study using optical biosensors of microcolorimetrical techniques
structural proteomics as NMR or fluorescence polarization study
the classification and prediction of structure or functional sites
in silico analysis of proteins and proteomes
increasing throughput and data quality for proteomics
Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, "Methods in Proteome and Protein Analysis" presents the most interesting papers from the 14th MPSA meeting.
Selected contributions discuss the latest techniques and approaches for analysing protein and proteome structures. Major topics include:
protein and peptide sample preparation and separationnew reagent for protein sequence analysis
mass spectrometry in protein research
analysis of posttranslational modification
protein-protein interaction using MALDI-MS
manipulation of genome or functional compositon trap
structure-function correlation study using optical biosensors of microcolorimetrical techniques
structural proteomics as NMR or fluorescence polarization study
the classification and prediction of structure or functional sites
in silico analysis of proteins and proteomes
increasing throughput and data quality for proteomics
Content:
Front Matter....Pages I-XXXII
Helix-Helix Packing Between Transmembrane Fragments....Pages 1-14
Mobility Studies in Proteins by 15N Nuclear Magnetic Resonance: Rusticyanin as an Example....Pages 15-33
Structure and Dynamics of Proteins in Crowded Media: A Time-Resolved Fluorescence Polarization Study....Pages 35-48
Analyses of Wheat Seed Proteome: Exploring Protein-Protein Interactions by Manipulating Genome Composition....Pages 49-66
Modification-Specific Proteomic Strategy for Identification of Glycosyl-Phosphatidylinositol Anchored Membrane Proteins....Pages 67-79
Diocleinae Lectins: Clues to Delineate Structure/Function Correlations....Pages 81-91
The Contribution of Optical Biosensors to the Analysis of Structure-Function Relationships in Proteins....Pages 93-101
The Use of Protein—protein Interaction Networks for Genome Wide Protein Function Comparisons and Predictions....Pages 103-124
Probing Ribosomal Proteins Capable of Interacting with Polyamines....Pages 125-132
Applications of Optical Biosensors to Structure-Function Studies on the EGF/EGF Receptor System....Pages 133-164
The Functional Interaction Trap: A Novel Strategy to Study Specific Protein-Protein Interactions....Pages 165-182
Analysis of Protein—Protein Interactions in Complex Biological Samples by MALDI TOF MS. Feasibility and Use of the Intensity-Fading (IF-) Approach....Pages 183-202
Accelerator Mass Spectrometry in Protein Analysis....Pages 203-216
The Quantitative Advantages of an Internal Standard in Multiplexing 2D Electrophoresis....Pages 217-230
Genetic Engineering of Bacterial and Eukaryotic Ribosomal Proteins for Investigation on Elongation Arrest of Nascent Polypeptides and Cell Differentiation....Pages 231-250
MALDI-MS Analysis of Peptides Modified with Photolabile Arylazido Groups....Pages 251-259
A New Edman-Type Reagent for High Sensitive Protein Sequencing....Pages 261-268
Amino Acid Sequencing of Sulfonic Acid-Labeled Tryptic Peptides Using Post-Source Decay and Quadratic Field MALDI-ToF Mass Spectrometry....Pages 269-278
Separation of Peptides and Amino Acids using High Performance Capillary Electrophoresis....Pages 279-297
InterPro and Proteome Analysis — In silico Analysis of Proteins and Proteomes....Pages 299-306
Prediction of Functional Sites in Proteins by Evolutionary Methods....Pages 307-318
Extracting and Searching for Structural Information: A Multiresolution Approach....Pages 319-340
Peak Erazor: A Windows-Based Program for Improving Peptide Mass Searches....Pages 341-357
Increasing Throughput and Data Quality for Proteomics....Pages 359-370
Back Matter....Pages 371-397
....Pages 399-404