Ebook: The Physiological Genomics of the Critically Ill Mouse

The physiological genomics of the cardiovascular system studies the relationship between gene and physiological (dys)function. It is a rapidly developing area of research and distinguishes itself from other areas of molecular medicine by its highly integrative nature. In this multi­ disciplinarian area of the physiological sciences, there is interaction between gene structure and physiological cardiovascular function as well as interactions between the different organs and their physiological compartments. The mouse has played a central role in the study of genomics due to the detailed knowledge of the mouse genome and the wide availability of genetically modified mice. In the past, the mouse had mainly been used in the area of immunology and molecular biology, and physiological interest in the mouse was scarce. As more insight has come into the structural genomics of the mouse, however, it has become increasingly important to understand the relation between gene and physiological function. With this in mind we have been organizing the Amsterdam Mouse Symposia to bring together different disciplines interested in the molecular basis of cardiovascular function (see J. of Clinical and Exp. Pharmacology and rd Physiology (2002) 29:A69-AI02 for the proceedings of the 3 Amsterdam Mouse Symposium and Basic Research in Cardiology (2000) 95:492-535 for nd the proceedings of the 2 symposium).

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This latest volume in the "Basic Science for the Cardiologist" book series is dedicated to the study of the physiological genomics of cardiovascular disease. It was inspired by the Third Amsterdam Mouse Symposium which showed the enormous strides that have been made in physiological genomics in recent years.
The integrative nature of this field has its best clinical analogue in critical care medicine where the cardiovascular system, and the diagnosis and treatment of the different organ systems and their successful interaction lie central. Physiological monitoring, mechanical ventilation, sedation and an integrative analysis all form part of the arsenal of the intensivist and also of the mouse physiologist.
Sections include the following: physiological monitoring of the mouse; cardiovascular pathophysiology of the mouse; and treating the critically ill mouse.

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This latest volume in the "Basic Science for the Cardiologist" book series is dedicated to the study of the physiological genomics of cardiovascular disease. It was inspired by the Third Amsterdam Mouse Symposium which showed the enormous strides that have been made in physiological genomics in recent years.
The integrative nature of this field has its best clinical analogue in critical care medicine where the cardiovascular system, and the diagnosis and treatment of the different organ systems and their successful interaction lie central. Physiological monitoring, mechanical ventilation, sedation and an integrative analysis all form part of the arsenal of the intensivist and also of the mouse physiologist.
Sections include the following: physiological monitoring of the mouse; cardiovascular pathophysiology of the mouse; and treating the critically ill mouse.
Content:
Front Matter....Pages i-xviii
Front Matter....Pages 1-1
Assessment of Quality in Commercially Supplied Genetically Modified Animals....Pages 3-9
Cannulation Techniques for Multiple Blood Withdrawal in Mice....Pages 11-21
Mouse Anesthesia in Relation to Optimal Hemodynamics....Pages 23-34
Mechanical Ventilation of Mice....Pages 35-51
Mouse Echocardiography....Pages 53-71
Optical Spectroscopy in Mice....Pages 73-88
ECG Monitoring and Analyses in Mice....Pages 89-100
High-Definition Magnetic Resonance of Genetically Modified Mice....Pages 101-117
Pet Studies in the Mouse....Pages 119-126
Mouse Telemetry for Long-Term Monitoring....Pages 127-141
Front Matter....Pages 143-143
Physiological Genomics Of Reperfusion Injury....Pages 145-158
Inos-Deficient Mice in the Study of Resuscitated Sepsis....Pages 159-177
The Microcirulation in Hypercholesterolemic Mice....Pages 179-186
The Mouse in Shock....Pages 187-200
Expression Systems to Analyze Transgenes in the Heart....Pages 201-210
Structural and Functional Adaptations of the Heart After Coronary Artery Ligation in the Mouse....Pages 211-224
Mouse Models of Sepsis....Pages 225-238
Mouse Models for Arterial Thrombosis: Does Clotting Make a Difference in Human Cardiovascular Disease?....Pages 239-255
Identification of Mouse Models of Cardiovascular Disease Using Mutagenesis....Pages 257-274
Acute Lung Injury and Pneumonia in Mice....Pages 275-288
Front Matter....Pages 289-289
Endothelial Nitric Oxide Synthase in Cardiovascular Homeostasis and Disease....Pages 291-310
Arteriogenesis in Mice....Pages 311-327
Mouse Models to Study Pro-and Antiangiogenic Potential: Novel Roles for PLGF and FLT1....Pages 329-347
Stem Cell Therapy in the Mouse Heart....Pages 349-361
Gene Therapy for the Critically Ill Mouse....Pages 363-381
Back Matter....Pages 383-386

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This latest volume in the "Basic Science for the Cardiologist" book series is dedicated to the study of the physiological genomics of cardiovascular disease. It was inspired by the Third Amsterdam Mouse Symposium which showed the enormous strides that have been made in physiological genomics in recent years.
The integrative nature of this field has its best clinical analogue in critical care medicine where the cardiovascular system, and the diagnosis and treatment of the different organ systems and their successful interaction lie central. Physiological monitoring, mechanical ventilation, sedation and an integrative analysis all form part of the arsenal of the intensivist and also of the mouse physiologist.
Sections include the following: physiological monitoring of the mouse; cardiovascular pathophysiology of the mouse; and treating the critically ill mouse.
Content:
Front Matter....Pages i-xviii
Front Matter....Pages 1-1
Assessment of Quality in Commercially Supplied Genetically Modified Animals....Pages 3-9
Cannulation Techniques for Multiple Blood Withdrawal in Mice....Pages 11-21
Mouse Anesthesia in Relation to Optimal Hemodynamics....Pages 23-34
Mechanical Ventilation of Mice....Pages 35-51
Mouse Echocardiography....Pages 53-71
Optical Spectroscopy in Mice....Pages 73-88
ECG Monitoring and Analyses in Mice....Pages 89-100
High-Definition Magnetic Resonance of Genetically Modified Mice....Pages 101-117
Pet Studies in the Mouse....Pages 119-126
Mouse Telemetry for Long-Term Monitoring....Pages 127-141
Front Matter....Pages 143-143
Physiological Genomics Of Reperfusion Injury....Pages 145-158
Inos-Deficient Mice in the Study of Resuscitated Sepsis....Pages 159-177
The Microcirulation in Hypercholesterolemic Mice....Pages 179-186
The Mouse in Shock....Pages 187-200
Expression Systems to Analyze Transgenes in the Heart....Pages 201-210
Structural and Functional Adaptations of the Heart After Coronary Artery Ligation in the Mouse....Pages 211-224
Mouse Models of Sepsis....Pages 225-238
Mouse Models for Arterial Thrombosis: Does Clotting Make a Difference in Human Cardiovascular Disease?....Pages 239-255
Identification of Mouse Models of Cardiovascular Disease Using Mutagenesis....Pages 257-274
Acute Lung Injury and Pneumonia in Mice....Pages 275-288
Front Matter....Pages 289-289
Endothelial Nitric Oxide Synthase in Cardiovascular Homeostasis and Disease....Pages 291-310
Arteriogenesis in Mice....Pages 311-327
Mouse Models to Study Pro-and Antiangiogenic Potential: Novel Roles for PLGF and FLT1....Pages 329-347
Stem Cell Therapy in the Mouse Heart....Pages 349-361
Gene Therapy for the Critically Ill Mouse....Pages 363-381
Back Matter....Pages 383-386
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