Ebook: Transgenic Crops of the World: Essential Protocols

Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.

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Transgenic Crops of the World is a state-of-the-art, compilation of the most up-to-date methods available for the genetic manipulation of the major crops of our world. These methods have been developed from research groups which have contributed significantly to the genetic improvement of their respective crops. A special 'Note' section is included to aid researchers to generate transgenic plants from 'new' or untested germplasms. This book is an essential guide for plant science courses at undergraduate and postgraduate levels and also for industrial biotechnology companies.

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Transgenic Crops of the World is a state-of-the-art, compilation of the most up-to-date methods available for the genetic manipulation of the major crops of our world. These methods have been developed from research groups which have contributed significantly to the genetic improvement of their respective crops. A special 'Note' section is included to aid researchers to generate transgenic plants from 'new' or untested germplasms. This book is an essential guide for plant science courses at undergraduate and postgraduate levels and also for industrial biotechnology companies.

Content:
Front Matter....Pages i-xii
Front Matter....Pages 1-1
Transgenic Rice Plants....Pages 3-18
Transformation of Wheat by Biolistics....Pages 19-34
Maize Transformation....Pages 35-44
Genetic Engineering of Oat (Avena Sativa L.) Via the Biolistic Bombardment of Shoot Apical Meristems....Pages 45-62
Generation of Transgenic Rye (Secale cereale L.) Plants with Single and Defined T-DNA Inserts, Following Agrobacterium-Mediated Gene Transfer....Pages 63-78
Sugarcane Transformation....Pages 79-88
Biolistic Transformation of Fescues and Ryegrasses....Pages 89-102
Front Matter....Pages 103-114
Transformation of Banana using Microprojectile Bombardment....Pages 115-128
Genetic Transformation of Tea....Pages 129-129
Microprojectile-Mediated Transformation of Pineapple....Pages 131-143
Regeneration and Genetic Transformation of Apple (Malus SPP.)....Pages 145-156
Genetic Transformation of Pear Via Agrobacterium-Mediated Gene Transfer....Pages 159-169
Front Matter....Pages 171-183
Genetic Transformation of Radish (Raphanus Sativus L.) by Floral-Dipping....Pages 187-197
Front Matter....Pages 199-214
Transformation of Carrot....Pages 217-228
Production of Transgenic Cassava (Manihot Esculenta Crantz)....Pages 229-242
Front Matter....Pages 243-254
Soybean Transformation Using the Agrobacterium-Mediated Cotyledonarynode Method....Pages 255-255
Gene Technology in Pea....Pages 257-269
Transformation of Cauliflower....Pages 271-280
Tomato Transformation — the Nuclear and Chloroplast Genomes....Pages 255-255
Genetic Transformation of Watermelon....Pages 281-290
Genetic Transformation of Sunflower (Helianthus Annuus L.)....Pages 291-300
Back Matter....Pages 301-319
....Pages 321-321