Ebook: FISH Technology

From the reviews:

"Ever wondered how to catch your FISH … in the dark pools of cellular biology? Then … FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) … . Every chapter is illustrated by several schematic drawings … . The manual is completed by a subject index … . The book is certainly clear … providing valuable protocols for both beginning FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)

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Content:
Front Matter....Pages I-X
Front Matter....Pages 1-1
FISH Techniques, FISH Probes and Their Applications in Medicine and Biology — An Overview....Pages 3-50
Microscopy and Imaging Systems....Pages 51-69
Front Matter....Pages 71-71
FISH on Chromosome Preparations of Peripheral Blood....Pages 73-81
FISH on Uncultured Blood or Bone Marrow....Pages 82-89
FISH on Blood or Bone Marrow Smears....Pages 90-96
Epithelial Cells from Buccal Smears and Urine....Pages 97-108
FISH on Hair Root Cells....Pages 109-114
Amniotic Fluid Cells — Uncultured....Pages 115-126
Human Sperm Cells....Pages 127-137
Front Matter....Pages 139-139
Archival Cytogenetic Slides....Pages 141-147
Formalin-Fixed and Paraffin-Embedded Tissue Sections....Pages 148-161
Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue....Pages 162-170
Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue....Pages 171-175
Nucleus Extraction from Cryofixed Tissue....Pages 176-184
Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells....Pages 185-196
Comparative Genomic Hybridization....Pages 197-217
Front Matter....Pages 219-219
High Resolution FISH of Stretched Chromosome Fibers....Pages 221-225
Quantitative DNA Fiber Mapping....Pages 226-253
PNA-FISH....Pages 254-261
Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH....Pages 262-271
Front Matter....Pages 219-219
The Combined Use of Nuclease in Situ Digestion and FISH (NU-FISH)....Pages 272-281
DNA Breakage Detection-FISH (DBD-FISH)....Pages 282-290
Detection of Radiosensitivity by Means of Three-Color FISH....Pages 291-300
Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T....Pages 301-306
Front Matter....Pages 307-307
Zoo-FISH....Pages 309-322
FISH on Insect Cells Transfected with Heterologous DNA....Pages 323-328
Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells....Pages 329-345
In Situ Detection of Viral Nucleic Acids by Chemiluminescence....Pages 346-359
Front Matter....Pages 361-361
Spectral Imaging Analysis of Metaphase and Interphase Cells....Pages 363-387
Microdissection of Chromosomes and Reverse FISH....Pages 388-407
Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements....Pages 408-424
Centromere-Specific Multicolor-FISH (cenM-FISH)....Pages 425-431
Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material....Pages 432-441
Simultaneous Fluorescence Immunophenotyping and FISH (FICTION)....Pages 442-460
Front Matter....Pages 461-461
PRINS: Hybridization and Primed in Situ Labeling in One Step....Pages 463-472
Microarrays and DNA-Chips in the Analysis of Genetic Aberrations....Pages 473-484
Back Matter....Pages 485-494

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Content:
Front Matter....Pages I-X
Front Matter....Pages 1-1
FISH Techniques, FISH Probes and Their Applications in Medicine and Biology — An Overview....Pages 3-50
Microscopy and Imaging Systems....Pages 51-69
Front Matter....Pages 71-71
FISH on Chromosome Preparations of Peripheral Blood....Pages 73-81
FISH on Uncultured Blood or Bone Marrow....Pages 82-89
FISH on Blood or Bone Marrow Smears....Pages 90-96
Epithelial Cells from Buccal Smears and Urine....Pages 97-108
FISH on Hair Root Cells....Pages 109-114
Amniotic Fluid Cells — Uncultured....Pages 115-126
Human Sperm Cells....Pages 127-137
Front Matter....Pages 139-139
Archival Cytogenetic Slides....Pages 141-147
Formalin-Fixed and Paraffin-Embedded Tissue Sections....Pages 148-161
Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue....Pages 162-170
Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue....Pages 171-175
Nucleus Extraction from Cryofixed Tissue....Pages 176-184
Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells....Pages 185-196
Comparative Genomic Hybridization....Pages 197-217
Front Matter....Pages 219-219
High Resolution FISH of Stretched Chromosome Fibers....Pages 221-225
Quantitative DNA Fiber Mapping....Pages 226-253
PNA-FISH....Pages 254-261
Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH....Pages 262-271
Front Matter....Pages 219-219
The Combined Use of Nuclease in Situ Digestion and FISH (NU-FISH)....Pages 272-281
DNA Breakage Detection-FISH (DBD-FISH)....Pages 282-290
Detection of Radiosensitivity by Means of Three-Color FISH....Pages 291-300
Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T....Pages 301-306
Front Matter....Pages 307-307
Zoo-FISH....Pages 309-322
FISH on Insect Cells Transfected with Heterologous DNA....Pages 323-328
Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells....Pages 329-345
In Situ Detection of Viral Nucleic Acids by Chemiluminescence....Pages 346-359
Front Matter....Pages 361-361
Spectral Imaging Analysis of Metaphase and Interphase Cells....Pages 363-387
Microdissection of Chromosomes and Reverse FISH....Pages 388-407
Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements....Pages 408-424
Centromere-Specific Multicolor-FISH (cenM-FISH)....Pages 425-431
Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material....Pages 432-441
Simultaneous Fluorescence Immunophenotyping and FISH (FICTION)....Pages 442-460
Front Matter....Pages 461-461
PRINS: Hybridization and Primed in Situ Labeling in One Step....Pages 463-472
Microarrays and DNA-Chips in the Analysis of Genetic Aberrations....Pages 473-484
Back Matter....Pages 485-494
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