Ebook: Topics in Fluorescence Spectroscopy

Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial three volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. These first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).

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This third volume details the applications of fluorescence spectroscopy in biochemistry and biophysics.

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This third volume details the applications of fluorescence spectroscopy in biochemistry and biophysics.
Content:
Front Matter....Pages i-xiv
Tyrosine Fluorescence and Phosphorescence from Proteins and Polypeptides....Pages 1-64
Fluorescence and Dynamics in Proteins....Pages 65-111
Tryptophan Phosphorescence from Proteins at Room Temperature....Pages 113-136
Fluorescence Studies of Nucleic Acids: Dynamics, Rigidities, and Structures....Pages 137-229
Fluorescence in Membranes....Pages 231-271
Fluorescence and Immunodiagnostic Methods....Pages 273-287
Total Internal Reflection Fluorescence....Pages 289-343
Microparticle Fluorescence and Energy Transfer....Pages 345-386
Back Matter....Pages 387-390

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This third volume details the applications of fluorescence spectroscopy in biochemistry and biophysics.
Content:
Front Matter....Pages i-xiv
Tyrosine Fluorescence and Phosphorescence from Proteins and Polypeptides....Pages 1-64
Fluorescence and Dynamics in Proteins....Pages 65-111
Tryptophan Phosphorescence from Proteins at Room Temperature....Pages 113-136
Fluorescence Studies of Nucleic Acids: Dynamics, Rigidities, and Structures....Pages 137-229
Fluorescence in Membranes....Pages 231-271
Fluorescence and Immunodiagnostic Methods....Pages 273-287
Total Internal Reflection Fluorescence....Pages 289-343
Microparticle Fluorescence and Energy Transfer....Pages 345-386
Back Matter....Pages 387-390
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