Ebook: Cryopreservation of Plant Germplasm II

Ex situ preservation of germplasm for higher plant species has been accom­ plished using either seeds or clones, but storage of these under typical condi­ tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur­ vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com­ monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not have occurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry­ opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.

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This volume highlights achievements in cryopreservation, chronicles method development, and describes relevant literature. The provided detailed information helps practitioners develop and improve methods for desired species. The volume is divided into four parts:
I. Cryopreservation of Germplasm;
II. Herbaceous Plants: Barley, celery, chamomile, chicory, garlic, ginseng, hop, horseradish, mint, taro, wasabi;
III. Woody Species: Coffee, Eucalyptus, guazuma, horse-chestnut, neem, olive, poplar, oak, Prunus, Ribes, rose.
IV. Australian Species.
Initially, cryopreservation was driven by the concern for loss of diversity of crops essential for continued improvement of the many plants used for food, health, and shelter. The interest has been expanded by conservationists and their concerns for retaining the diversity of natural populations.

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This volume highlights achievements in cryopreservation, chronicles method development, and describes relevant literature. The provided detailed information helps practitioners develop and improve methods for desired species. The volume is divided into four parts:
I. Cryopreservation of Germplasm;
II. Herbaceous Plants: Barley, celery, chamomile, chicory, garlic, ginseng, hop, horseradish, mint, taro, wasabi;
III. Woody Species: Coffee, Eucalyptus, guazuma, horse-chestnut, neem, olive, poplar, oak, Prunus, Ribes, rose.
IV. Australian Species.
Initially, cryopreservation was driven by the concern for loss of diversity of crops essential for continued improvement of the many plants used for food, health, and shelter. The interest has been expanded by conservationists and their concerns for retaining the diversity of natural populations.
Content:
Front Matter....Pages I-XVI
Front Matter....Pages 1-1
Cryopreservation of Plant Germplasm: Introduction and Some Observations....Pages 3-21
Implementing Cryopreservation for Long-Term Germplasm Preservation in Vegetatively Propagated Species....Pages 22-33
Front Matter....Pages 35-35
Cryopreservation of Allium sativum L. (Garlic)....Pages 37-47
Cryopreservation of Apium graveolens L. (Celery) Seeds....Pages 48-56
Cryopreservation of Armoracia rusticana P. Gaert., B. Mey. et Scherb. (Horseradish) Hairy Root Cultures....Pages 57-65
Cryopreservation of Chamomilla recutita L. Rauschert (Chamomile) Callus....Pages 66-77
Cryopreservation of Cichorium intybus L. var. foliosum (Chicory)....Pages 78-95
Cryopreservation of Colocasia esculenta L. Schott (Taro)....Pages 96-118
Cryopreservation of Hordeum (Barley)....Pages 119-135
Cryopreservation of Humulus lupulus L. (Hop)....Pages 136-150
Cryopreservation of Mentha (Mint)....Pages 151-163
Cryopreservation of Panax (Ginseng)....Pages 164-179
Cryopreservation of In Vitro Grown Apical Shoot Tips of Wasabia japonica (Wasabi) by Different Procedures....Pages 180-195
Front Matter....Pages 197-197
Cryopreservation of Somatic Embryos from Aesculus hippocastanum L. (Horse Chestnut)....Pages 199-212
Cryopreservation of Neem (Azadirachta indica A. Juss.) Seeds....Pages 213-219
Cryopreservation of Coffea (Coffee)....Pages 220-233
Cryopreservation of Eucalyptus sp. Shoot Tips by the Encapsulation-Dehydration Procedure....Pages 234-245
Cryopreservation of Guazuma crinita Mart. (Guazuma)....Pages 246-256
Cryopreservation of Olea europaea L. (Olive)....Pages 257-268
Cryopreservation of Germplasm of Populus (Poplar) Species....Pages 269-286
Front Matter....Pages 197-197
Cryopreservation of Quercus (Oak) Species....Pages 287-311
Cryopreservation of Rosa (Rose)....Pages 312-322
Front Matter....Pages 323-343
Cryostorage of Somatic Tissues of Endangered Australian Species....Pages 344-353
Cryopreservation of Australian Species — The Role of Plant Growth Regulators....Pages 355-355
Back Matter....Pages 357-372
....Pages 373-390