Ebook: Insect Cell Culture: Fundamental and Applied Aspects

`In summary, this book covers a wide range of aspects relating to insect cell culture and the baculovirus expression system. It is an authoritative volume which is clearly and conci8sely written by experts selected from industry and academia and is to be highly recommended.'
Entomologia Experimentalis et Applicata, 87 (1998)
`This is an authoritative reference text written by leading experts in their respective fields of insects cell culture. The book targets a potential audience ranging from students and researchers in academia through to those concerned in industry with biotechnology applications.'
SGM Quarterly, August (1997)

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Insect Cell Cultures: Fundamental and Applied Aspects is a comprehensive reference work, covering the key issues involved in nearly 30 review papers: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; economics and regulatory aspects.
For cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.

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Insect Cell Cultures: Fundamental and Applied Aspects is a comprehensive reference work, covering the key issues involved in nearly 30 review papers: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; economics and regulatory aspects.
For cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.
Content:
Front Matter....Pages ii-vii
Development and characterization of insect cell lines....Pages 3-11
New approaches to insect tissue culture....Pages 13-22
Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene....Pages 23-31
Insect cell physiology....Pages 33-41
Insect cell cultivation: growth and kinetics....Pages 43-56
Medium design for insect cell culture....Pages 57-70
Baculovirus-insect cell interactions....Pages 73-93
Replication patterns and cytopathology of cells infected with baculoviruses....Pages 95-110
Construction of baculovirus recombinants....Pages 111-123
Passage effect of virus infection in insect cells....Pages 125-137
Post-translational modifications in insect cells....Pages 139-144
Productivity of insect cells for recombinant proteins....Pages 145-148
Chaperone and foldase coexpression in the baculovirus-insect cell expression system....Pages 149-159
Shear sensitivity of insect cells....Pages 163-171
Insect cell bioreactors....Pages 173-189
Perfusion bioreactors for the production of recombinant proteins in insect cells....Pages 191-198
Immobilization of insect cells....Pages 199-208
Modelling baculovirus infection of insect cells in culture....Pages 209-219
Scale up aspects of sparged insect-cell bioreactors....Pages 221-229
Oxygen gradients in small and big sparged insect-cell bioreactors....Pages 231-238
Downstream processing of insect cell cultures....Pages 239-257
Parvovirus diagnostics and vaccine production in insect cells....Pages 261-270
Classical swine fever virus diagnostics and vaccine production in insect cells....Pages 271-277
Production of multidomain complement glycoproteins in insect cells....Pages 279-288
Economics of baculovirus-insect cell production systems....Pages 291-297
Safety aspects of insect cell culture....Pages 299-304
Regulatory issues in the use of insect-cell culture....Pages 305-309
Back Matter....Pages 310-315

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Insect Cell Cultures: Fundamental and Applied Aspects is a comprehensive reference work, covering the key issues involved in nearly 30 review papers: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; economics and regulatory aspects.
For cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.
Content:
Front Matter....Pages ii-vii
Development and characterization of insect cell lines....Pages 3-11
New approaches to insect tissue culture....Pages 13-22
Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene....Pages 23-31
Insect cell physiology....Pages 33-41
Insect cell cultivation: growth and kinetics....Pages 43-56
Medium design for insect cell culture....Pages 57-70
Baculovirus-insect cell interactions....Pages 73-93
Replication patterns and cytopathology of cells infected with baculoviruses....Pages 95-110
Construction of baculovirus recombinants....Pages 111-123
Passage effect of virus infection in insect cells....Pages 125-137
Post-translational modifications in insect cells....Pages 139-144
Productivity of insect cells for recombinant proteins....Pages 145-148
Chaperone and foldase coexpression in the baculovirus-insect cell expression system....Pages 149-159
Shear sensitivity of insect cells....Pages 163-171
Insect cell bioreactors....Pages 173-189
Perfusion bioreactors for the production of recombinant proteins in insect cells....Pages 191-198
Immobilization of insect cells....Pages 199-208
Modelling baculovirus infection of insect cells in culture....Pages 209-219
Scale up aspects of sparged insect-cell bioreactors....Pages 221-229
Oxygen gradients in small and big sparged insect-cell bioreactors....Pages 231-238
Downstream processing of insect cell cultures....Pages 239-257
Parvovirus diagnostics and vaccine production in insect cells....Pages 261-270
Classical swine fever virus diagnostics and vaccine production in insect cells....Pages 271-277
Production of multidomain complement glycoproteins in insect cells....Pages 279-288
Economics of baculovirus-insect cell production systems....Pages 291-297
Safety aspects of insect cell culture....Pages 299-304
Regulatory issues in the use of insect-cell culture....Pages 305-309
Back Matter....Pages 310-315
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