Ebook: Chromosome Painting: Principles, Strategies and Scope

Chromosome Painting is the most modern and novel technique for directly identifying several gene sequences simultaneously in the chromosome, with the aid of specific probes in molecular hybridization. Its resolution ranges from single copy to entire genome sequences. It is now applied in plant, animal, and human systems, in gene mapping, identification of genetic disorders, evolutionary studies, and gene transfer experiments. This treatise is the first of its kind to cover the technique with all its modifications and applications. It is designed for regular use by postgraduate students and research workers in cell and molecular genetics, plant and animal sciences, agriculture, medicine, and phylogenetic studies.

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Chromosome Painting is the most modern and novel technique for directly identifying several gene sequences simultaneously in the chromosome, with the aid of specific probes in molecular hybridization. Its resolution ranges from single copy to entire genome sequences. It is now applied in plant, animal, and human systems, in gene mapping, identification of genetic disorders, evolutionary studies, and gene transfer experiments. This treatise is the first of its kind to cover the technique with all its modifications and applications. It is designed for regular use by postgraduate students and research workers in cell and molecular genetics, plant and animal sciences, agriculture, medicine, and phylogenetic studies.

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Chromosome Painting is the most modern and novel technique for directly identifying several gene sequences simultaneously in the chromosome, with the aid of specific probes in molecular hybridization. Its resolution ranges from single copy to entire genome sequences. It is now applied in plant, animal, and human systems, in gene mapping, identification of genetic disorders, evolutionary studies, and gene transfer experiments. This treatise is the first of its kind to cover the technique with all its modifications and applications. It is designed for regular use by postgraduate students and research workers in cell and molecular genetics, plant and animal sciences, agriculture, medicine, and phylogenetic studies.
Content:
Front Matter....Pages i-ix
Chromosome painting — principles, strategies and scope....Pages 1-5
Human chromosomal banding by in situ hybridization of isochores....Pages 7-15
Telomere length measurement by Q-FISH....Pages 17-22
Refined characterisation of chromosome aberrations in tumours by multicolour banding and electronic mapping resources....Pages 23-28
Relationship of telomere sequence and constitutive heterochromatin in the human and apes as detected by PRINS....Pages 29-35
Chromosome painting in farm, pet and wild animal species....Pages 37-55
Chromosome painting in plants....Pages 57-69
Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS....Pages 71-82
GISH technology in plant genome research....Pages 83-104
The use of FISH in chromosomal localization of transgenes in rice....Pages 105-113
Microdissection and chromosome painting of plant B chromosomes....Pages 115-124
Chromosome painting as a tool for rice genetics and breeding....Pages 125-132
Fluorescent in situ hybridization in plant polytene chromosomes....Pages 133-138
Molecular cytogenetics of introgressive hybridization in plants....Pages 139-148
High resolution FISH to delineate contiguous and small DNA sequences....Pages 149-154
Identification and analysis of sex chromosomes by comparative genomic hybridization (CGH)....Pages 155-161
Comparative karyotype using bidirectional chromosome painting: how and why?....Pages 163-170
Immunolocalization of HP1 proteins in metaphasic mammalian chromosomes....Pages 171-174
Back Matter....Pages 175-179

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Chromosome Painting is the most modern and novel technique for directly identifying several gene sequences simultaneously in the chromosome, with the aid of specific probes in molecular hybridization. Its resolution ranges from single copy to entire genome sequences. It is now applied in plant, animal, and human systems, in gene mapping, identification of genetic disorders, evolutionary studies, and gene transfer experiments. This treatise is the first of its kind to cover the technique with all its modifications and applications. It is designed for regular use by postgraduate students and research workers in cell and molecular genetics, plant and animal sciences, agriculture, medicine, and phylogenetic studies.
Content:
Front Matter....Pages i-ix
Chromosome painting — principles, strategies and scope....Pages 1-5
Human chromosomal banding by in situ hybridization of isochores....Pages 7-15
Telomere length measurement by Q-FISH....Pages 17-22
Refined characterisation of chromosome aberrations in tumours by multicolour banding and electronic mapping resources....Pages 23-28
Relationship of telomere sequence and constitutive heterochromatin in the human and apes as detected by PRINS....Pages 29-35
Chromosome painting in farm, pet and wild animal species....Pages 37-55
Chromosome painting in plants....Pages 57-69
Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS....Pages 71-82
GISH technology in plant genome research....Pages 83-104
The use of FISH in chromosomal localization of transgenes in rice....Pages 105-113
Microdissection and chromosome painting of plant B chromosomes....Pages 115-124
Chromosome painting as a tool for rice genetics and breeding....Pages 125-132
Fluorescent in situ hybridization in plant polytene chromosomes....Pages 133-138
Molecular cytogenetics of introgressive hybridization in plants....Pages 139-148
High resolution FISH to delineate contiguous and small DNA sequences....Pages 149-154
Identification and analysis of sex chromosomes by comparative genomic hybridization (CGH)....Pages 155-161
Comparative karyotype using bidirectional chromosome painting: how and why?....Pages 163-170
Immunolocalization of HP1 proteins in metaphasic mammalian chromosomes....Pages 171-174
Back Matter....Pages 175-179
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