Ebook: Techniques in Animal Cytogenetics
- Tags: Cell Biology, Human Genetics, Biochemistry general, Human Physiology
- Series: Principles and Practice
- Year: 2000
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
A better "casting" could not be conceived. The authors of this book are gold smiths on the subject. I have followed their work since their "entry" into cyto genetics and I have a high esteem for them. I consider it an honour to be asked to write the preface of their opus. Paul Popescu, Directeur de Recherche at INRA, has also played a promi nent part in the development of animal cytogenetics, especially in domestic animals. He is able to tell you the cost of a translocation in a pig breeding farm or a cow population: a fortune! P. Popescu has played a great part in gene mapping of these species using "in situ DNA hybridisation". His contributions are recognised world-wide. His laboratory receives many visitors every year and it serves as a reference for domestic animal cytogenetics. Helene Hayes, Charge de Recherche at INRA, has collaborated with P. POPESCU in the elaboration of the "at hand" techniques and in many other discoveries which are listed in her bibliography. She showed the fascinating correspondence between bovine and human chromosomes and the com pared gene maps of domestic bovidae.
The theoretical background of cytogenetics as well as the practical methods to investigate chromosomes from Drosophila to mammals and humans are presented in this book. Theory and practical protocols on the following topics are included: Cell culture methods, chromosome preparation and banding techniques, radioactive and fluorescent in situ hybridisation (FISH) methods and their derivates (chromosome painting), meiotic chromosome methods using light and electronic microscopy, the polytenic (Drosophila) and lampbrush (urodeles) chromosomes as well as flow and slit scan cytometry.
The editors, all at INRA, played a prominent part in the development of cytogenetics. Paul Popescu, a specialist for domestic animals worked with gene mapping, Helene Hayes, together with Popescu showed the homology of bovine and human chromosome disorders, and Bernard Dutrilaux focused on human cytogenetics.
The theoretical background of cytogenetics as well as the practical methods to investigate chromosomes from Drosophila to mammals and humans are presented in this book. Theory and practical protocols on the following topics are included: Cell culture methods, chromosome preparation and banding techniques, radioactive and fluorescent in situ hybridisation (FISH) methods and their derivates (chromosome painting), meiotic chromosome methods using light and electronic microscopy, the polytenic (Drosophila) and lampbrush (urodeles) chromosomes as well as flow and slit scan cytometry.
The editors, all at INRA, played a prominent part in the development of cytogenetics. Paul Popescu, a specialist for domestic animals worked with gene mapping, Helene Hayes, together with Popescu showed the homology of bovine and human chromosome disorders, and Bernard Dutrilaux focused on human cytogenetics.
Content:
Front Matter....Pages I-XXVI
Preparation of Chromosome Spreads....Pages 1-24
Chromosome Banding Techniques....Pages 25-68
In Situ Hybridisation Techniques....Pages 69-83
Methods of Germ Cells Study....Pages 85-102
The Lampbrush Chromosomes of Amphibians....Pages 103-136
Drosophila Chromosome Study Techniques....Pages 137-149
Techniques of Interphasic Nucleus Study....Pages 151-155
Application of Flow Cytometry and Slit-Scan Fluorometry in Mammalian Chromosomes Analysis and Sorting....Pages 157-186
Back Matter....Pages 187-229
The theoretical background of cytogenetics as well as the practical methods to investigate chromosomes from Drosophila to mammals and humans are presented in this book. Theory and practical protocols on the following topics are included: Cell culture methods, chromosome preparation and banding techniques, radioactive and fluorescent in situ hybridisation (FISH) methods and their derivates (chromosome painting), meiotic chromosome methods using light and electronic microscopy, the polytenic (Drosophila) and lampbrush (urodeles) chromosomes as well as flow and slit scan cytometry.
The editors, all at INRA, played a prominent part in the development of cytogenetics. Paul Popescu, a specialist for domestic animals worked with gene mapping, Helene Hayes, together with Popescu showed the homology of bovine and human chromosome disorders, and Bernard Dutrilaux focused on human cytogenetics.
Content:
Front Matter....Pages I-XXVI
Preparation of Chromosome Spreads....Pages 1-24
Chromosome Banding Techniques....Pages 25-68
In Situ Hybridisation Techniques....Pages 69-83
Methods of Germ Cells Study....Pages 85-102
The Lampbrush Chromosomes of Amphibians....Pages 103-136
Drosophila Chromosome Study Techniques....Pages 137-149
Techniques of Interphasic Nucleus Study....Pages 151-155
Application of Flow Cytometry and Slit-Scan Fluorometry in Mammalian Chromosomes Analysis and Sorting....Pages 157-186
Back Matter....Pages 187-229
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