Ebook: Flow Cytometry and Cell Sorting

Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible.
From the reviews of the 1st edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended and well worth purchasing." (Cytobios, GB)
"Full practical details and useful hints are given. This book will prove to be very useful in the lab." (Comp Biochem Physiol A, GB)
"..the book provides a good overview of many of the uses of flow cytometry and would certainly be a good starting point for any of the topics covered. It is easy to read and as such would provide a good introduction to newcomers to flow cytometry." (Austr J Med Science)

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Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible.
From the reviews of the 1st edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended and well worth purchasing." (Cytobios, GB)
"Full practical details and useful hints are given. This book will prove to be very useful in the lab." (Comp Biochem Physiol A, GB)
"..the book provides a good overview of many of the uses of flow cytometry and would certainly be a good starting point for any of the topics covered. It is easy to read and as such would provide a good introduction to newcomers to flow cytometry." (Austr J Med Science)

---

Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible.
From the reviews of the 1st edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended and well worth purchasing." (Cytobios, GB)
"Full practical details and useful hints are given. This book will prove to be very useful in the lab." (Comp Biochem Physiol A, GB)
"..the book provides a good overview of many of the uses of flow cytometry and would certainly be a good starting point for any of the topics covered. It is easy to read and as such would provide a good introduction to newcomers to flow cytometry." (Austr J Med Science)
Content:
Front Matter....Pages I-XXIII
Front Matter....Pages 1-1
Operation of a Flow Cytometer....Pages 3-25
Front Matter....Pages 27-27
Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies....Pages 29-37
Immunofluorescence: Basic Considerations....Pages 38-52
Multicolor Immunofluorescence Analysis....Pages 53-62
Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells....Pages 63-70
Cytometry and Sorting of Live Cells According to Their Secreted Products....Pages 71-76
Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes....Pages 77-81
Scatchard Analysis by Flow Cytometry....Pages 82-85
Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry....Pages 86-97
Front Matter....Pages 99-99
Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis....Pages 101-104
Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression....Pages 105-111
High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique....Pages 112-124
Front Matter....Pages 125-125
Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium....Pages 127-139
Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry....Pages 140-158
Biochemical Parameters of Cell Function....Pages 159-182
The FACS-Gal Assay....Pages 183-185
Ligand Acidification by Nonadherent Cells....Pages 186-193
Front Matter....Pages 195-195
Powerful Preselection....Pages 197-209
Magnetic Selection of Transfected Cells....Pages 210-217
High Gradient Magnetic Cell Sorting....Pages 218-247
Front Matter....Pages 195-195
Setup of a Flow Sorter....Pages 248-254
Sorting of Rare Cells....Pages 255-265
Fluorescence-Activated Chromosome Sorting....Pages 266-276
Analysis and Sorting of Plant Chromosomes by Flow Cytometry....Pages 277-292
Large Particle Sorting....Pages 293-317
Front Matter....Pages 319-319
Biological and Laser Safety....Pages 321-325
Back Matter....Pages 327-355

---

Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible.
From the reviews of the 1st edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended and well worth purchasing." (Cytobios, GB)
"Full practical details and useful hints are given. This book will prove to be very useful in the lab." (Comp Biochem Physiol A, GB)
"..the book provides a good overview of many of the uses of flow cytometry and would certainly be a good starting point for any of the topics covered. It is easy to read and as such would provide a good introduction to newcomers to flow cytometry." (Austr J Med Science)
Content:
Front Matter....Pages I-XXIII
Front Matter....Pages 1-1
Operation of a Flow Cytometer....Pages 3-25
Front Matter....Pages 27-27
Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies....Pages 29-37
Immunofluorescence: Basic Considerations....Pages 38-52
Multicolor Immunofluorescence Analysis....Pages 53-62
Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells....Pages 63-70
Cytometry and Sorting of Live Cells According to Their Secreted Products....Pages 71-76
Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes....Pages 77-81
Scatchard Analysis by Flow Cytometry....Pages 82-85
Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry....Pages 86-97
Front Matter....Pages 99-99
Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis....Pages 101-104
Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression....Pages 105-111
High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique....Pages 112-124
Front Matter....Pages 125-125
Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium....Pages 127-139
Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry....Pages 140-158
Biochemical Parameters of Cell Function....Pages 159-182
The FACS-Gal Assay....Pages 183-185
Ligand Acidification by Nonadherent Cells....Pages 186-193
Front Matter....Pages 195-195
Powerful Preselection....Pages 197-209
Magnetic Selection of Transfected Cells....Pages 210-217
High Gradient Magnetic Cell Sorting....Pages 218-247
Front Matter....Pages 195-195
Setup of a Flow Sorter....Pages 248-254
Sorting of Rare Cells....Pages 255-265
Fluorescence-Activated Chromosome Sorting....Pages 266-276
Analysis and Sorting of Plant Chromosomes by Flow Cytometry....Pages 277-292
Large Particle Sorting....Pages 293-317
Front Matter....Pages 319-319
Biological and Laser Safety....Pages 321-325
Back Matter....Pages 327-355
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