Ebook: Studies of Aging

Interest in the study of the biology of aging (Gerontology), especially as it relates to humans (Geriatrics), has increased steadily in the last 50 years and continues today to be at the forefront of experimental biology. Due to the many recent advances in this area, a large variety of techniques are available and selection of the best experimental approach may be difficult. It is the purpose of this laboratory manual to provide the researcher with a specific group of techniques with emphasis on practical, hands-on information.
A variety of protocols using molecular, cellular and physiological techniques are presented in detail and appropriate animal models have been selected for the study of aging and aging-related diseases. Non-invasive in vivo techniques are described for the study of Alzheimer's dementia and cardiovascular diseases. Techniques for detection of oxidative processes are presented to explore the role of free radicals in causing cell damage and mutations as well as techniques to study apoptosis (programmed cell death) for its role in cell death and aging processes.

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Interest in the study of the biology of aging (Gerontology), especially as it relates to humans (Geriatrics), has increased steadily in the last 50 years and continues today to be at the forefront of experimental biology. Due to the many recent advances in this area, a large variety of techniques are available and selection of the best experimental approach may be difficult. It is the purpose of this laboratory manual to provide the researcher with a specific group of techniques with emphasis on practical, hands-on information.
A variety of protocols using molecular, cellular and physiological techniques are presented in detail and appropriate animal models have been selected for the study of aging and aging-related diseases. Non-invasive in vivo techniques are described for the study of Alzheimer's dementia and cardiovascular diseases. Techniques for detection of oxidative processes are presented to explore the role of free radicals in causing cell damage and mutations as well as techniques to study apoptosis (programmed cell death) for its role in cell death and aging processes.
Content:
Front Matter....Pages I-XI
Front Matter....Pages 1-1
Genotypic Selection of Age-Related DNA Rearrangements by PCR....Pages 3-14
Measurement of Antioxidants in Cutaneous Tissues....Pages 15-32
Front Matter....Pages 33-33
Cellular Aging / Replicative Senescence....Pages 35-45
Methods for Evaluating a Slice Culture Model of Alzheimer’s Disease....Pages 46-57
Cell Death....Pages 58-77
Method For Studying the Migration of Aging Vascular Smooth Muscle Cells....Pages 78-89
Sensitized Chemiluminescence and Fluorescence Methods in Studies of Oxidative Stress....Pages 90-101
Front Matter....Pages 103-103
How to Choose an Animal Model....Pages 105-110
Measuring Mortality and Reproduction in Large Cohorts of the Mediterranean Fruit Fly....Pages 111-124
Front Matter....Pages 125-125
Methods for Evaluating in Vivo Rodent Models for Alzheimer’s Disease....Pages 127-142
Dietary Restriction in Aging....Pages 143-163
Methods For the Evaluation of the Hypothalamo-Pituitary-Adrenocortical Axis in the Aged Rat....Pages 164-170
Assessing Blood Replacement Fluids For Organ Preservation and Low Temperature Surgery....Pages 171-181
Brain Imaging in Normal Aging and in Alzheimer’s Disease....Pages 182-206
Back Matter....Pages 207-208

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Interest in the study of the biology of aging (Gerontology), especially as it relates to humans (Geriatrics), has increased steadily in the last 50 years and continues today to be at the forefront of experimental biology. Due to the many recent advances in this area, a large variety of techniques are available and selection of the best experimental approach may be difficult. It is the purpose of this laboratory manual to provide the researcher with a specific group of techniques with emphasis on practical, hands-on information.
A variety of protocols using molecular, cellular and physiological techniques are presented in detail and appropriate animal models have been selected for the study of aging and aging-related diseases. Non-invasive in vivo techniques are described for the study of Alzheimer's dementia and cardiovascular diseases. Techniques for detection of oxidative processes are presented to explore the role of free radicals in causing cell damage and mutations as well as techniques to study apoptosis (programmed cell death) for its role in cell death and aging processes.
Content:
Front Matter....Pages I-XI
Front Matter....Pages 1-1
Genotypic Selection of Age-Related DNA Rearrangements by PCR....Pages 3-14
Measurement of Antioxidants in Cutaneous Tissues....Pages 15-32
Front Matter....Pages 33-33
Cellular Aging / Replicative Senescence....Pages 35-45
Methods for Evaluating a Slice Culture Model of Alzheimer’s Disease....Pages 46-57
Cell Death....Pages 58-77
Method For Studying the Migration of Aging Vascular Smooth Muscle Cells....Pages 78-89
Sensitized Chemiluminescence and Fluorescence Methods in Studies of Oxidative Stress....Pages 90-101
Front Matter....Pages 103-103
How to Choose an Animal Model....Pages 105-110
Measuring Mortality and Reproduction in Large Cohorts of the Mediterranean Fruit Fly....Pages 111-124
Front Matter....Pages 125-125
Methods for Evaluating in Vivo Rodent Models for Alzheimer’s Disease....Pages 127-142
Dietary Restriction in Aging....Pages 143-163
Methods For the Evaluation of the Hypothalamo-Pituitary-Adrenocortical Axis in the Aged Rat....Pages 164-170
Assessing Blood Replacement Fluids For Organ Preservation and Low Temperature Surgery....Pages 171-181
Brain Imaging in Normal Aging and in Alzheimer’s Disease....Pages 182-206
Back Matter....Pages 207-208
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