Ebook: A Molecular and Cellular View of Protein Kinase CK2

It is now generally recognized that protein kinase signaling is involved in virtually every aspect of cell function, including growth and proliferation. The field of protein phosphorylation, including the enzymes involved in this post-translational modification, continues to advance at a fascinating pace.
Since the first international meeting on this topic, held in Heidelberg in 1994, several new avenues of CK2 research have emerged despite persistent deficiencies in our understanding of the regulation of its activity. Among the significant new directions are studies related to the structure of the enzyme, especially its crystal structure, as well as an interesting aspect of CK2 function that involves its subunits as binding partners of several other proteins. In addition, new data have been gathered on the role of CK2 in transcription as well as in certain other cellular functions. To address these various aspects of the progress of CK2, a number of key scientists from different parts of the world came together at the second international meeting on `A Molecular and Cellular View of Protein Kinase CK2', held at Villard de Lans near Grenoble on September 24-26, 1997. The meeting was attended by nearly 50 participants and included 28 presentations, which provide a view of the latest progress on protein kinase CK2.

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It is now generally recognized that protein kinase signaling is involved in virtually every aspect of cell function, including growth and proliferation. The field of protein phosphorylation, including the enzymes involved in this post-translational modification, continues to advance at a fascinating pace.
Since the first international meeting on this topic, held in Heidelberg in 1994, several new avenues of CK2 research have emerged despite persistent deficiencies in our understanding of the regulation of its activity. Among the significant new directions are studies related to the structure of the enzyme, especially its crystal structure, as well as an interesting aspect of CK2 function that involves its subunits as binding partners of several other proteins. In addition, new data have been gathered on the role of CK2 in transcription as well as in certain other cellular functions. To address these various aspects of the progress of CK2, a number of key scientists from different parts of the world came together at the second international meeting on `A Molecular and Cellular View of Protein Kinase CK2', held at Villard de Lans near Grenoble on September 24-26, 1997. The meeting was attended by nearly 50 participants and included 28 presentations, which provide a view of the latest progress on protein kinase CK2.

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It is now generally recognized that protein kinase signaling is involved in virtually every aspect of cell function, including growth and proliferation. The field of protein phosphorylation, including the enzymes involved in this post-translational modification, continues to advance at a fascinating pace.
Since the first international meeting on this topic, held in Heidelberg in 1994, several new avenues of CK2 research have emerged despite persistent deficiencies in our understanding of the regulation of its activity. Among the significant new directions are studies related to the structure of the enzyme, especially its crystal structure, as well as an interesting aspect of CK2 function that involves its subunits as binding partners of several other proteins. In addition, new data have been gathered on the role of CK2 in transcription as well as in certain other cellular functions. To address these various aspects of the progress of CK2, a number of key scientists from different parts of the world came together at the second international meeting on `A Molecular and Cellular View of Protein Kinase CK2', held at Villard de Lans near Grenoble on September 24-26, 1997. The meeting was attended by nearly 50 participants and included 28 presentations, which provide a view of the latest progress on protein kinase CK2.
Content:
Front Matter....Pages i-2
CK2, a protein kinase of the next millennium....Pages 3-12
A multifunctional network of basic residues confers unique properties to protein kinase CK2....Pages 13-19
Intermolecular contact sites in protein kinase CK2....Pages 21-28
Binding of polylysine to protein kinase CK2, measured by Surface Plasmon Resonance....Pages 29-33
Functional analysis of CK2?-derived synthetic fragments....Pages 35-42
Dissecting subdomains involved in multiple functions of the CK2? subunit....Pages 43-50
Interactions of protein kinase CK2? subunit within the holoenzyme and with other proteins....Pages 51-58
CK2? loci in the human genome: Structure and transcriptional activity....Pages 59-64
Murine protein kinase CK2: Gene and oncogene....Pages 65-74
Interactions of protein kinase CK2 subunits....Pages 75-83
Mutations in the C-terminal domain of topoisomerase II affect meiotic function and interaction with the casein kinase 2 ? subunit....Pages 85-95
Association of protein kinase CK2 with eukaryotic translation initiation factor eIF-2 and with grp94/endoplasmin....Pages 97-104
Searching interaction partners of protein kinase CK2? subunit by two-hybrid screening....Pages 105-109
Protein kinase CK2 interacts with a multi-protein binding domain of p53....Pages 111-120
BTF3 is a potential new substrate of protein kinase CK2....Pages 121-128
Protein kinase CK2? may induce gene expression but unlikely acts directly as a DNA-binding transcription-activating factor....Pages 129-134
Role of protein kinase CK2 in phosphorylation of nucleosomal proteins in relation to transcriptional activity....Pages 135-142
A review of progress towards elucidating the role of protein kinase CK2 in polymerase III transcription: Regulation of the TATA binding protein....Pages 143-148
The binding of the ? subunit of protein kinase CK2 and RAP74 subunit of TFIIF to protein-coding genes in living cells is DRB sensitive....Pages 149-159
Transcriptional regulation of the S. cerevisiae ENA1 gene by casein kinase II....Pages 161-167
A role for casein kinase II phosphorylation in the regulation of IRF-1 transcriptional activity....Pages 169-180
A structural model for elongation factor 1 (EF-1) and phosphorylation by protein kinase CKII....Pages 181-186
Protein kinase CK2-dependent regulation of p53 function: Evidence that the phosphorylation status of the serine 386 (CK2) site of p53 is constitutive and stable....Pages 187-199
Distribution of CK2, its substrate MAP1B and phosphatases in neuronal cells....Pages 201-205
CK2? — protein phosphatase 2A molecular complex: Possible interaction with the MAP kinase pathway....Pages 207-212
Expression and regulation of protein kinase CK2 during the cell cycle....Pages 213-222
Identification of proteins that associate with protein kinase CK2....Pages 223-228
Multiple forms of protein kinase CK2 present in leukemic cells: In vitro study of its origin by proteolysis....Pages 229-234
Back Matter....Pages 235-239

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It is now generally recognized that protein kinase signaling is involved in virtually every aspect of cell function, including growth and proliferation. The field of protein phosphorylation, including the enzymes involved in this post-translational modification, continues to advance at a fascinating pace.
Since the first international meeting on this topic, held in Heidelberg in 1994, several new avenues of CK2 research have emerged despite persistent deficiencies in our understanding of the regulation of its activity. Among the significant new directions are studies related to the structure of the enzyme, especially its crystal structure, as well as an interesting aspect of CK2 function that involves its subunits as binding partners of several other proteins. In addition, new data have been gathered on the role of CK2 in transcription as well as in certain other cellular functions. To address these various aspects of the progress of CK2, a number of key scientists from different parts of the world came together at the second international meeting on `A Molecular and Cellular View of Protein Kinase CK2', held at Villard de Lans near Grenoble on September 24-26, 1997. The meeting was attended by nearly 50 participants and included 28 presentations, which provide a view of the latest progress on protein kinase CK2.
Content:
Front Matter....Pages i-2
CK2, a protein kinase of the next millennium....Pages 3-12
A multifunctional network of basic residues confers unique properties to protein kinase CK2....Pages 13-19
Intermolecular contact sites in protein kinase CK2....Pages 21-28
Binding of polylysine to protein kinase CK2, measured by Surface Plasmon Resonance....Pages 29-33
Functional analysis of CK2?-derived synthetic fragments....Pages 35-42
Dissecting subdomains involved in multiple functions of the CK2? subunit....Pages 43-50
Interactions of protein kinase CK2? subunit within the holoenzyme and with other proteins....Pages 51-58
CK2? loci in the human genome: Structure and transcriptional activity....Pages 59-64
Murine protein kinase CK2: Gene and oncogene....Pages 65-74
Interactions of protein kinase CK2 subunits....Pages 75-83
Mutations in the C-terminal domain of topoisomerase II affect meiotic function and interaction with the casein kinase 2 ? subunit....Pages 85-95
Association of protein kinase CK2 with eukaryotic translation initiation factor eIF-2 and with grp94/endoplasmin....Pages 97-104
Searching interaction partners of protein kinase CK2? subunit by two-hybrid screening....Pages 105-109
Protein kinase CK2 interacts with a multi-protein binding domain of p53....Pages 111-120
BTF3 is a potential new substrate of protein kinase CK2....Pages 121-128
Protein kinase CK2? may induce gene expression but unlikely acts directly as a DNA-binding transcription-activating factor....Pages 129-134
Role of protein kinase CK2 in phosphorylation of nucleosomal proteins in relation to transcriptional activity....Pages 135-142
A review of progress towards elucidating the role of protein kinase CK2 in polymerase III transcription: Regulation of the TATA binding protein....Pages 143-148
The binding of the ? subunit of protein kinase CK2 and RAP74 subunit of TFIIF to protein-coding genes in living cells is DRB sensitive....Pages 149-159
Transcriptional regulation of the S. cerevisiae ENA1 gene by casein kinase II....Pages 161-167
A role for casein kinase II phosphorylation in the regulation of IRF-1 transcriptional activity....Pages 169-180
A structural model for elongation factor 1 (EF-1) and phosphorylation by protein kinase CKII....Pages 181-186
Protein kinase CK2-dependent regulation of p53 function: Evidence that the phosphorylation status of the serine 386 (CK2) site of p53 is constitutive and stable....Pages 187-199
Distribution of CK2, its substrate MAP1B and phosphatases in neuronal cells....Pages 201-205
CK2? — protein phosphatase 2A molecular complex: Possible interaction with the MAP kinase pathway....Pages 207-212
Expression and regulation of protein kinase CK2 during the cell cycle....Pages 213-222
Identification of proteins that associate with protein kinase CK2....Pages 223-228
Multiple forms of protein kinase CK2 present in leukemic cells: In vitro study of its origin by proteolysis....Pages 229-234
Back Matter....Pages 235-239
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