Ebook: Practical Plant Virology: Protocols and Exercises

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Content:
Front Matter....Pages I-XVI
Front Matter....Pages 1-4
Mechanical Inoculation of Plants....Pages 5-13
Inoculation of Protoplasts Isolated from Leaves....Pages 14-28
Differential Temperature Treatment of Infected Plants....Pages 29-31
Front Matter....Pages 32-33
Description of External Symptoms....Pages 35-41
Determination of Internal Symptoms....Pages 42-47
Systemic Acquired Resistance (SAR)....Pages 48-56
Demonstration of Phloem Necrosis in Plants Affected by Potato Leafroll....Pages 57-59
Front Matter....Pages 60-62
Infectivity Assay on Local-Lesion Hosts....Pages 63-64
Infectivity Assay on Hosts Producing Starch Lesions....Pages 65-65
Infectivity Assay on Hosts Producing Systemic Symptoms Only....Pages 67-77
Effect of Temperature on the Susceptibility of Plants....Pages 78-81
Effect of Abrasives on the Infection....Pages 82-85
Effect of Inhibitors on the Infection....Pages 86-87
Effect of Light on the Susceptibility of Plants....Pages 88-89
Effect of Time of the Day on the Susceptibility of Plants....Pages 90-91
Front Matter....Pages 92-93
Determination of the Dilution End-Point....Pages 94-95
Determination of the Thermal Inactivation Point....Pages 97-99
Determination of the Longevity In Vitro....Pages 100-101
Front Matter....Pages 102-104
Determination of the Dilution End-Point....Pages 105-107
Determination of the Thermal Inactivation Point....Pages 97-99
Determination of the Longevity In Vitro....Pages 108-109
Front Matter....Pages 110-110
Virus Transmission by Grafting....Pages 111-111
Virus Transmission Through Soil....Pages 113-115
Virus Transmission by Nematodes....Pages 116-124
Virus Transmission by Fungi....Pages 125-127
Virus Transmission by Aphids....Pages 128-142
Transmission by Leafhoppers (Cicadellidae)....Pages 143-147
Transmission by Whiteflies (Aleyrodidae)....Pages 148-158
Transmission by Thrips (Thripidae)....Pages 159-168
Wedge-Grafting of Tobacco....Pages 169-173
Separation of a Nonpersistently and a Persistently Transmitted Virus by Differential Transmission....Pages 174-177
Visualisation of Salivary Sheaths Formed by Aphids....Pages 178-179
Separation of a Semipersistently Transmitted and a Circulative-Propagative Virus by Differential Transmission....Pages 180-183
Front Matter....Pages 184-185
Freezing....Pages 186-187
Dehydration....Pages 189-191
Freeze-Drying....Pages 192-193
Short-Term Storage....Pages 194-197
Front Matter....Pages 198-199
Heat Treatment....Pages 200-201
Front Matter....Pages 203-206
Meristem-Tip Culture....Pages 207-208
Heat Treatment Combined With Meristem-Tip Culture....Pages 203-206
Front Matter....Pages 209-215
Density-Gradient Centrifugation....Pages 216-217
Virus Purification Protocols....Pages 219-231
Front Matter....Pages 232-237
UV Light Absorption....Pages 238-273
Buoyant Density in Caesium Chloride....Pages 275-275
Particle Morphology and Size as Determined with the Electron Microscope....Pages 277-283
Front Matter....Pages 284-290
Determination of the Molecular Mass of Coat Proteins by Gel Electrophoresis....Pages 291-297
RNA Extraction from Purified Virus Particles....Pages 299-301
Determination of the Molecular Mass of Ribonucleic Acids by Gel Electrophoresis....Pages 302-307
Front Matter....Pages 308-311
Production of Antiserum....Pages 312-315
Absorption of Antiserum....Pages 317-321
Chloroplast Agglutination Test....Pages 322-328
Microprecipitin Test....Pages 329-332
Gel Double-Diffusion Test in Plates....Pages 333-335
Enzyme-Linked Immunosorbent Assay....Pages 336-340
Immunoblotting Assays....Pages 341-347
Standard DAS-ELISA....Pages 348-362
Front Matter....Pages 363-372
Electron Microscope Serology....Pages 373-375
Light Microscope Serology....Pages 377-379
Front Matter....Pages 380-391
Northern Blotting....Pages 392-400
Northern Hybridisation....Pages 401-403
Dot-Blot Hybridisation (Nucleic Acid Spot Hybridisation)....Pages 404-407
Polymerase Chain Reaction....Pages 408-412
Diagnosis of a Disease and Identification of the Causal Virus(es)....Pages 413-414
Back Matter....Pages 415-425
....Pages 427-434