Ebook: Nucleic Acid Electrophoresis

Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.

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Content:
Front Matter....Pages I-XIII
DNA Gel Electrophoresis....Pages 1-53
Denaturating Electrophoresis in DNA Sequencing: A Brief History and Current Protocols....Pages 54-98
An Introduction to DNA Fingerprinting Using RFLP and RAPD Techniques....Pages 99-128
Pulsed-Field Gel Electrophoresis for Genomic Fingerprinting of Pathogenic Bacteria....Pages 129-151
Ultrasensitive Silver Based Stains for Nucleic Acid Detection....Pages 152-161
The Analysis of Microsatellites by Silver Staining of Nondenaturing Polyacrylamide Gels....Pages 162-169
Capillary Electrophoretic Separation of DNA Fragments....Pages 170-192
Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis....Pages 193-201
Electrophoresis in the Field of Forensic Science....Pages 202-238
The Electrophoretic Mobility Shift Assay (EMSA) for Detection and Analysis of Protein-DNA Interactions....Pages 239-271
Mobility Shift Analysis of Protein-DNA Complexes by Capillary Electrophoresis....Pages 272-291
Polyacrylamide Gel Electrophoresis of DNA/Protein Complexes....Pages 292-310
Detection of DNA Curvature Using Transverse Pore Gradient Polyacrylamide Gel Electrophoresis....Pages 311-324
Back Matter....Pages 325-328

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Content:
Front Matter....Pages I-XIII
DNA Gel Electrophoresis....Pages 1-53
Denaturating Electrophoresis in DNA Sequencing: A Brief History and Current Protocols....Pages 54-98
An Introduction to DNA Fingerprinting Using RFLP and RAPD Techniques....Pages 99-128
Pulsed-Field Gel Electrophoresis for Genomic Fingerprinting of Pathogenic Bacteria....Pages 129-151
Ultrasensitive Silver Based Stains for Nucleic Acid Detection....Pages 152-161
The Analysis of Microsatellites by Silver Staining of Nondenaturing Polyacrylamide Gels....Pages 162-169
Capillary Electrophoretic Separation of DNA Fragments....Pages 170-192
Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis....Pages 193-201
Electrophoresis in the Field of Forensic Science....Pages 202-238
The Electrophoretic Mobility Shift Assay (EMSA) for Detection and Analysis of Protein-DNA Interactions....Pages 239-271
Mobility Shift Analysis of Protein-DNA Complexes by Capillary Electrophoresis....Pages 272-291
Polyacrylamide Gel Electrophoresis of DNA/Protein Complexes....Pages 292-310
Detection of DNA Curvature Using Transverse Pore Gradient Polyacrylamide Gel Electrophoresis....Pages 311-324
Back Matter....Pages 325-328
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