Ebook: Animal Cell Culture Techniques

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

---

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

---

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Content:
Front Matter....Pages I-XIII
Front Matter....Pages 1-1
Cloning Animal Cells....Pages 3-12
Cell Culture and Diagnostic Virology....Pages 13-37
Screening for Mycoplasma Contamination in Animal Cell Cultures....Pages 38-53
Serum-Free Media....Pages 54-66
Screening Culture Media and Sera for Endotoxin Testing....Pages 67-81
Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry....Pages 82-104
The Generation of Long-Term Human Antibody-Producing Cell Lines Using the Epstein-Barr Virus....Pages 105-114
Primary Culture....Pages 115-128
Front Matter....Pages 129-129
Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes....Pages 131-148
Methods for Studying Phosphoinositide Metabolism in Cultured Cells....Pages 149-169
Analysis of Cell Cycle and Cell Death Mechanisms....Pages 170-193
Front Matter....Pages 195-195
Assessing Release of Secretory Products from Individual Cells....Pages 197-216
The Granulocyte/Macrophage Colony-Forming Unit Assay....Pages 217-230
Investigations into Cell Differentiation Using Cells in Culture....Pages 231-239
Cell Culture Models for Mononuclear Phagocytes....Pages 240-263
The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses....Pages 264-297
Cell Culture Models for Polarized Epithelial Monolayers....Pages 298-318
Ion Channels and Cell Signaling in Cell Cultures....Pages 319-356
Isolation, Cultivation and Differentiation of Lung Type II Epithelial Cells....Pages 357-370
Culture Systems for Hepatocytes for Use in Toxicology and Differentiation Studies....Pages 371-392
Front Matter....Pages 393-393
Cellular Models for In Vitro Toxicity Testing....Pages 395-422
Miniaturized In Vitro Methods in Toxicity Testing....Pages 423-433
Investigation of Anti-tumor Drug Accumulation and Efflux in Cell Culture....Pages 434-448
Current Status of Genetically Engineered In Vitro Systems for Biotransformation....Pages 449-456
Front Matter....Pages 457-457
Scale-Up of Animal Cell Cultures....Pages 459-474
Purification of Monoclonal Antibodies Produced by Animal Cells in Culture....Pages 475-486
Biosafety Assessment of Cultured Animal Cells Used for Biopharmaceutical Production....Pages 487-520
Cytokine Cell Culture Assays....Pages 521-541
Front Matter....Pages 543-543
Antisense Methods in Cell Culture....Pages 545-554
Transfection with Ribozymes to Investigate the Role of Specific Gene Expression....Pages 555-566
Methods for Studying the Transcription and DNase I Hypersensitive Sites of Genes in Cells in Culture....Pages 567-581
Analysis of Specific and Differentially Expressed mRNAs in Cell Culture....Pages 582-599
Human Gene Therapy: Review and Outlook....Pages 600-612
Back Matter....Pages 613-618

---

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Content:
Front Matter....Pages I-XIII
Front Matter....Pages 1-1
Cloning Animal Cells....Pages 3-12
Cell Culture and Diagnostic Virology....Pages 13-37
Screening for Mycoplasma Contamination in Animal Cell Cultures....Pages 38-53
Serum-Free Media....Pages 54-66
Screening Culture Media and Sera for Endotoxin Testing....Pages 67-81
Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry....Pages 82-104
The Generation of Long-Term Human Antibody-Producing Cell Lines Using the Epstein-Barr Virus....Pages 105-114
Primary Culture....Pages 115-128
Front Matter....Pages 129-129
Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes....Pages 131-148
Methods for Studying Phosphoinositide Metabolism in Cultured Cells....Pages 149-169
Analysis of Cell Cycle and Cell Death Mechanisms....Pages 170-193
Front Matter....Pages 195-195
Assessing Release of Secretory Products from Individual Cells....Pages 197-216
The Granulocyte/Macrophage Colony-Forming Unit Assay....Pages 217-230
Investigations into Cell Differentiation Using Cells in Culture....Pages 231-239
Cell Culture Models for Mononuclear Phagocytes....Pages 240-263
The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses....Pages 264-297
Cell Culture Models for Polarized Epithelial Monolayers....Pages 298-318
Ion Channels and Cell Signaling in Cell Cultures....Pages 319-356
Isolation, Cultivation and Differentiation of Lung Type II Epithelial Cells....Pages 357-370
Culture Systems for Hepatocytes for Use in Toxicology and Differentiation Studies....Pages 371-392
Front Matter....Pages 393-393
Cellular Models for In Vitro Toxicity Testing....Pages 395-422
Miniaturized In Vitro Methods in Toxicity Testing....Pages 423-433
Investigation of Anti-tumor Drug Accumulation and Efflux in Cell Culture....Pages 434-448
Current Status of Genetically Engineered In Vitro Systems for Biotransformation....Pages 449-456
Front Matter....Pages 457-457
Scale-Up of Animal Cell Cultures....Pages 459-474
Purification of Monoclonal Antibodies Produced by Animal Cells in Culture....Pages 475-486
Biosafety Assessment of Cultured Animal Cells Used for Biopharmaceutical Production....Pages 487-520
Cytokine Cell Culture Assays....Pages 521-541
Front Matter....Pages 543-543
Antisense Methods in Cell Culture....Pages 545-554
Transfection with Ribozymes to Investigate the Role of Specific Gene Expression....Pages 555-566
Methods for Studying the Transcription and DNase I Hypersensitive Sites of Genes in Cells in Culture....Pages 567-581
Analysis of Specific and Differentially Expressed mRNAs in Cell Culture....Pages 582-599
Human Gene Therapy: Review and Outlook....Pages 600-612
Back Matter....Pages 613-618
....