Ebook: Aspartic Proteinases: Retroviral and Cellular Enzymes

The VIIth International Conference on Aspartic Proteinases was held in Banff, Alberta, Canada, from October 22 to 27, 1996. The venue was the Banff Centre in the Canadian Rockies, a setting well known worldwide for the scenic beauty and mountain grandeur. It was perhaps presumptuous of the organizers to call this the seventh Aspartic Proteinase Conference but it was felt that the meeting in 1982, organized by Tom Blundell and John Kay, was of an international stature and covered topics sufficiently broad to constitute a conference. Thus, there is a discontinuity in that the Gifu Conference organized by Prof. Kenji Takahashi was the fifth International Conference on Aspartic Proteinases. Officially, there has not been a sixth Conference and if there is confusion, it is the result of my desire to recognize the importance of the London meeting. Banffhosted 106 scientists from 14 different countries. There were 26 invited speak­ ers among the 44 oral presentations of the 7 main sessions. In addition, there were 53 con­ tributed poster presentations that spanned the whole range of interest in aspartic proteinases.

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Plenary: The Aspartic Proteinases: An Historical Overview; T.L.Blundell, et al.Retroviral Aspartic Proteinases I: The Regulation of Sequential Processing of HIV-1 Gag by the Viral Protease; S.C. Pettit,et al. A Cellular Anti-Apoptosis Protein Is Cleaved by the HIV-1 Protease; B.D.Korant, et al.Retroviral Aspartic Proteinases II:InVitro Selection and Characterization of VX-478 Resistant HIV-1 Variants; S. Pazhanisamy, et al. Sensitivity to Inhibition and Catalytic Efficiency of HIV Proteinase Mutants; S.I. Wilson et al.Mammalian Aspartic Proteinases: Comparison of the Specificity of the Aspartic Proteinases Towards Internally Consistent Sets of Oligopeptide Substrates; B.M. Dunn, et al.Aspartic Proenzymes andActivation: Proteolytic and Non-Proteolytic Activation of Prorenin; T.L. Reudelhuber, et al.Microbial Aspartic Proteinases:Aspergillusniger Acid Proteinase A-Structure and Function; K. Takahashi,et al.Parasite Aspartic Proteinase: Structural Analysis of Plasmespin II: A Comparison with Human Aspartic Proteases; A.M. Silva.PlantAspartic Proteinases: Structural and Functional Aspects of Cardosins; C. Faro, et al. 86 Additional Articles. Index.

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Plenary: The Aspartic Proteinases: An Historical Overview; T.L.Blundell, et al.Retroviral Aspartic Proteinases I: The Regulation of Sequential Processing of HIV-1 Gag by the Viral Protease; S.C. Pettit,et al. A Cellular Anti-Apoptosis Protein Is Cleaved by the HIV-1 Protease; B.D.Korant, et al.Retroviral Aspartic Proteinases II:InVitro Selection and Characterization of VX-478 Resistant HIV-1 Variants; S. Pazhanisamy, et al. Sensitivity to Inhibition and Catalytic Efficiency of HIV Proteinase Mutants; S.I. Wilson et al.Mammalian Aspartic Proteinases: Comparison of the Specificity of the Aspartic Proteinases Towards Internally Consistent Sets of Oligopeptide Substrates; B.M. Dunn, et al.Aspartic Proenzymes andActivation: Proteolytic and Non-Proteolytic Activation of Prorenin; T.L. Reudelhuber, et al.Microbial Aspartic Proteinases:Aspergillusniger Acid Proteinase A-Structure and Function; K. Takahashi,et al.Parasite Aspartic Proteinase: Structural Analysis of Plasmespin II: A Comparison with Human Aspartic Proteases; A.M. Silva.PlantAspartic Proteinases: Structural and Functional Aspects of Cardosins; C. Faro, et al. 86 Additional Articles. Index.
Content:
Front Matter....Pages i-xvii
The Aspartic Proteinases....Pages 1-13
The Regulation of Sequential Processing of HIV-1 Gag by the Viral Protease....Pages 15-25
A Cellular Anti-Apoptosis Protein is Cleaved by the HIV-1 Protease....Pages 27-29
Engineering Catalytically Defective Forms of HIV Protease to Modulate Its Activity....Pages 31-39
The Aspartic Proteinase from Equine Infectious Anaemia Virus....Pages 41-45
The Effect of Substrates on the Kinetics and the in Vivo Threshold Activity of Mutant HIV-1 Proteases....Pages 47-51
A Comparison of gag—pol Precursor Cleavage in Naturally Arising HIV Variants....Pages 53-57
X-Ray Crystallographic Studies of the Structure-Function Relationships of HIV-1 Protease....Pages 59-63
Optimization of a Macromolecular Inhibitor of HIV-1 Protease....Pages 65-70
Back Matter....Pages 71-74
Sensitivity to Inhibition and Catalytic Efficiency of HIV Proteinase Mutants....Pages 75-83
Computer Assisted Evaluation of Inhibitor Sensitivity to HIV-1 PR Mutants....Pages 85-89
Mechanism of Action of Aspartic Proteases....Pages 91-97
Theory and Method of a Priori Computation of Catalytic Acts of Aspartic and Serine Proteinases....Pages 99-103
Back Matter....Pages 105-108
Comparison of The Specificity of the Aspartic Proteinases Towards Internally Consistent Sets of Oligopeptide Substrates....Pages 109-113
Expression of Chimeric Human Aspartic Proteinases....Pages 115-121
Cathepsin E....Pages 123-126
Back Matter....Pages 127-132
Biosynthesis and Intracellular Targeting of the Lysosomal Aspartic Proteinase Cathepsin D....Pages 133-138
An Aspartic Proteinase Expressed in the Equine Placenta....Pages 139-146
Protein Engineering Aspartic Proteinases....Pages 147-152
Post X-Ray Crystallographic Studies of Chymosin Specificity....Pages 223-228
Epitope Mapping of Recombinant Human Procathepsin D....Pages 153-162
Construction of Chimeric Enzymes to Probe Subsite Contributions to Catalytic Specificity....Pages 163-167
Expression of Multiple Genes for Pregnancy-Associated Glycoproteins in the Sheep Placenta....Pages 169-177
Mass Spectrometry as a Tool for Studying the Action of Human Aspartic Proteases on Peptides and Proteins....Pages 179-184
Skin Surface Proteolytic Activity....Pages 185-189
Cathepsin E in the Central Nervous System....Pages 191-194
Detailed Analysis of Human Cathepsin E Prime Region Specificity....Pages 195-200
Back Matter....Pages 201-206
Proteolytic and Non-Proteolytic Activation of Prorenin....Pages 207-212
The Roles of the Basic Residues in the Prosegment of Aspergillopepsinogen I....Pages 213-217
Activation Mechanism of Pepsinogen as Compared to the Processing of HIV Protease gag-pol Precursor Protein....Pages 219-222
Cardosinogen A....Pages 223-228
Crystal Structure of Human Pepsinogen A....Pages 229-238
Crystallographic Studies of an Activation Intermediate of Human Gastricsin....Pages 239-244
Back Matter....Pages 245-252
Crystal Structure of The Rhizomucor miehei Aspartic Proteinase....Pages 253-258
Back Matter....Pages 259-263
Yapsin1....Pages 265-270
Bacterial Aspartic Proteinases as Novel Antibiotic Targets....Pages 271-273
Structural Thermodynamic Study of the Binding of Renin Inhibitors to Endothiapepsin....Pages 275-282
Development and Testing of Inhibitors of Candida Aspartic Proteinases....Pages 283-292
Functional Aspects of Secreted Candida Proteinases....Pages 293-296
Substrate Specificity of Non-Pepsin-Type Acid Proteinase, Aspergillus niger Proteinase A....Pages 361-362
Pepstatin-Insensitive Carboxyl Proteinases from Prokaryotes....Pages 297-313
Overcoming the Unfavourable Entropic Contribution of Ligand Binding with a Macrocyclic Inhibitor Bound to Penicillopepsin....Pages 315-319
Back Matter....Pages 321-324
Structural Analysis of Plasmepsin II....Pages 325-328
Screening Aspartyl Proteases with Combinatorial Libraries....Pages 329-333
Thermodynamics and Proton Uptake for Pepstatin Binding to Retroviral and Eukaryotic Aspartic Proteases....Pages 335-338
Studies on Plasmepsins I and II from the Malarial Parasite Plasmodium falciparum and their Exploitation as Drug Targets....Pages 339-344
Back Matter....Pages 345-348
Structural and Functional Aspects of Cardosins....Pages 349-353
Aspartic Proteinases and Inhibitors in Plant Pathogenesis....Pages 355-359
Back Matter....Pages 361-362
Crystallisation, Structure Solution, and Initial Refinement of Plant Cardosin-A....Pages 363-373
Acid Proteinase From Nepenthes distillatoria (Badura)....Pages 375-380
A Comparative Study on the Aspartic Proteinases from Different Species of Cynara....Pages 381-386
Substrate Specificity and Molecular Modelling of Aspartic Proteinases (Cyprosins) from Flowers of Cynara cardunculus SUBSP. flavescens CV. Cardoon....Pages 387-389
Back Matter....Pages 391-395
Back Matter....Pages 397-406
....Pages 407-411