Ebook: African Horse Sickness

African horse sickness virus is a double-stranded RNA virus which causes a non-contagious, infectious arthropod-borne disease of equines and occasionally dogs. Nine distinct, internationally recognised serotypes of the virus have so far been identified. This book is based upon the findings of two programmes funded by the European Commission. It will be of value not only to the specialist research workers but also to veterinary workers dealing with control and to legislators seeking to promote safe international movement of equines. The topics covered include state-of-the-art discussions on diagnostics, vaccines, molecular biology, vector studies, and epidemiology.

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African horse sickness virus is a double-stranded RNA virus which causes a non-contagious, infectious arthropod-borne disease of equines and occasionally dogs. Nine distinct, internationally recognised serotypes of the virus have so far been identified. This book is based upon the findings of two programmes funded by the European Commission. It will be of value not only to the specialist research workers but also to veterinary workers dealing with control and to legislators seeking to promote safe international movement of equines. The topics covered include state-of-the-art discussions on diagnostics, vaccines, molecular biology, vector studies, and epidemiology.

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African horse sickness virus is a double-stranded RNA virus which causes a non-contagious, infectious arthropod-borne disease of equines and occasionally dogs. Nine distinct, internationally recognised serotypes of the virus have so far been identified. This book is based upon the findings of two programmes funded by the European Commission. It will be of value not only to the specialist research workers but also to veterinary workers dealing with control and to legislators seeking to promote safe international movement of equines. The topics covered include state-of-the-art discussions on diagnostics, vaccines, molecular biology, vector studies, and epidemiology.
Content:
Front Matter....Pages I-VIII
Front Matter....Pages 1-1
Taxonomy of African horse sickness viruses....Pages 3-11
Epidemiology of African horse sickness and the role of the zebra in South Africa....Pages 13-19
Transmission and distribution of African horse sickness virus serotypes in South African zebra....Pages 21-28
Serological and virological responses in mules and donkeys following inoculation with African horse sickness virus serotype 4....Pages 29-36
Donkeys as reservoirs of African horse sickness virus....Pages 37-47
Clinical, virological and immune responses of normal and immunosuppressed donkeys (Equus asinus africanus) after inoculation with African horse sickness virus....Pages 49-56
Immunohistochemical demonstration of African horse sickness viral antigen in tissues of experimentally infected equines....Pages 57-65
Front Matter....Pages 67-67
The 1996 outbreak of African horse sickness in South Africa — the entomological perspective....Pages 69-83
The seasonal and geographical distribution of Culicoides imicola, C. pulicaris group and C. obsoletus group biting midges in central and southern Spain....Pages 85-91
The relationship between climate and the distribution of Culicoides imicola in Iberia....Pages 93-102
Simulation studies of African horse sickness in Spain....Pages 103-111
The Culicoides vectors of African horse sickness virus in Morocco: distribution and epidemiological implications....Pages 113-125
Studies of the mortality rate of Culicoides imicola in Morocco....Pages 127-136
Modelling the distribution and abundance of Culicoides imicola in Morocco and Iberia using climatic data and satellite imagery....Pages 137-153
Duration of repellency of various synthetic and plant-derived preparations for Culicoides imicola, the vector of African horse sickness virus....Pages 155-163
Front Matter....Pages 165-174
New generation of African horse sickness virus vaccines based on structural and molecular studies of the virus particles....Pages 175-175
Expression of the major core structural proteins VP3 and VP7 of African horse sickness virus, and production of core-like particles....Pages 177-202
Molecular epidemiology of African horse sickness virus based on analyses and comparisons of genome segments 7 and 10....Pages 203-209
Front Matter....Pages 211-219
Structural studies of orbivirus particles....Pages 221-234
Characterization of two African horse sickness virus nonstructural proteins, NS1 and NS3....Pages 175-175
Development of a mouse model system, coding assignments and identification of the genome segments controlling virulence of African horse sickness virus serotypes 3 and 8....Pages 235-250
Phylogenetic analysis of African horse sickness virus segment 10: sequence variation, virulence characteristics and cell exit....Pages 251-258
Front Matter....Pages 259-279
Future international management of African horse sickness vaccines....Pages 281-293
Application of an indirect fluorescent antibody assay for the detection of African horse sickness virus antibodies....Pages 295-295
Validation of ELISA for the detection of African horse sickness virus antigens and antibodies....Pages 297-304
Use of reverse transcriptase-polymerase chain reaction (RT-PCR) and dot-blot hybridisation for the detection and identification of African horse sickness virus nucleic acids....Pages 305-310
Western immunoblotting as a method for the detection of African horse sickness virus protein-specific antibodies: Differentiation between infected and vaccinated horses....Pages 311-315
Conclusions and recommendations of the meeting....Pages 317-327
Back Matter....Pages 329-336
....Pages 337-342