Ebook: Cell Culture Engineering VI

The latest edition in this continuing series includes the newest advances in the rapidly evolving field of animal cell culture, genetic manipulations for heterologous gene expression, cell line enhancements, improved bioreactor designs and separations, gene therapy manufacturing, tissue engineering, anti-apoptosis strategies and cell cycle research. The contents include new research articles as well as critical reviews on emerging topics such as viral and viral-like agent contamination of animal cell culture components. These papers were carefully selected from contributions by leading academic and industrial experts in the biotechnology community at the recent Cell Culture Engineering VI Meeting in San Diego, USA, 1998. However, the book is not merely a proceedings.
Audience: Biochemical engineers, cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment and the biotechnology or pharmaceutical industry.

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The latest edition in this continuing series includes the newest advances in the rapidly evolving field of animal cell culture, genetic manipulations for heterologous gene expression, cell line enhancements, improved bioreactor designs and separations, gene therapy manufacturing, tissue engineering, anti-apoptosis strategies and cell cycle research. The contents include new research articles as well as critical reviews on emerging topics such as viral and viral-like agent contamination of animal cell culture components. These papers were carefully selected from contributions by leading academic and industrial experts in the biotechnology community at the recent Cell Culture Engineering VI Meeting in San Diego, USA, 1998. However, the book is not merely a proceedings.
Audience: Biochemical engineers, cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment and the biotechnology or pharmaceutical industry.

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The latest edition in this continuing series includes the newest advances in the rapidly evolving field of animal cell culture, genetic manipulations for heterologous gene expression, cell line enhancements, improved bioreactor designs and separations, gene therapy manufacturing, tissue engineering, anti-apoptosis strategies and cell cycle research. The contents include new research articles as well as critical reviews on emerging topics such as viral and viral-like agent contamination of animal cell culture components. These papers were carefully selected from contributions by leading academic and industrial experts in the biotechnology community at the recent Cell Culture Engineering VI Meeting in San Diego, USA, 1998. However, the book is not merely a proceedings.
Audience: Biochemical engineers, cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment and the biotechnology or pharmaceutical industry.
Content:
Front Matter....Pages i-vi
Cell therapy in kidney failure....Pages 1-8
Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE)....Pages 9-17
Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination....Pages 19-29
Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line....Pages 31-42
Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them....Pages 43-48
An overview of viral and viral-like agents in cell culture systems....Pages 49-52
New adenovirus vectors for protein production and gene transfer....Pages 53-64
Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue....Pages 65-72
Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth — associated production of a foreign protein, ?-galactosidase....Pages 73-80
A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy....Pages 81-89
Recombinant insulin-like growth factor-I (IGF-I) production in Super-CHO results in the expression of IGF-I receptor and IGF binding protein 3....Pages 91-99
Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions....Pages 101-109
Regulated multicistronic expression technology for mammalian metabolic engineering....Pages 111-125
Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions....Pages 127-138
Historical reflections on cell culture engineering....Pages 139-145
Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation....Pages 147-155
Population balance model of in vivo neutrophil formation following bone marrow rescue therapy....Pages 157-162
Mammalian cell retention devices for stirred perfusion bioreactors....Pages 163-175
Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells....Pages 177-188
Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions....Pages 189-203
Effect of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures....Pages 205-211
Effects of CO2 and osmolality on hybridoma cells: growth, metabolism and monoclonal antibody production....Pages 213-227
pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons....Pages 229-235

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The latest edition in this continuing series includes the newest advances in the rapidly evolving field of animal cell culture, genetic manipulations for heterologous gene expression, cell line enhancements, improved bioreactor designs and separations, gene therapy manufacturing, tissue engineering, anti-apoptosis strategies and cell cycle research. The contents include new research articles as well as critical reviews on emerging topics such as viral and viral-like agent contamination of animal cell culture components. These papers were carefully selected from contributions by leading academic and industrial experts in the biotechnology community at the recent Cell Culture Engineering VI Meeting in San Diego, USA, 1998. However, the book is not merely a proceedings.
Audience: Biochemical engineers, cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment and the biotechnology or pharmaceutical industry.
Content:
Front Matter....Pages i-vi
Cell therapy in kidney failure....Pages 1-8
Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE)....Pages 9-17
Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination....Pages 19-29
Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line....Pages 31-42
Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them....Pages 43-48
An overview of viral and viral-like agents in cell culture systems....Pages 49-52
New adenovirus vectors for protein production and gene transfer....Pages 53-64
Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue....Pages 65-72
Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth — associated production of a foreign protein, ?-galactosidase....Pages 73-80
A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy....Pages 81-89
Recombinant insulin-like growth factor-I (IGF-I) production in Super-CHO results in the expression of IGF-I receptor and IGF binding protein 3....Pages 91-99
Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions....Pages 101-109
Regulated multicistronic expression technology for mammalian metabolic engineering....Pages 111-125
Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions....Pages 127-138
Historical reflections on cell culture engineering....Pages 139-145
Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation....Pages 147-155
Population balance model of in vivo neutrophil formation following bone marrow rescue therapy....Pages 157-162
Mammalian cell retention devices for stirred perfusion bioreactors....Pages 163-175
Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells....Pages 177-188
Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions....Pages 189-203
Effect of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures....Pages 205-211
Effects of CO2 and osmolality on hybridoma cells: growth, metabolism and monoclonal antibody production....Pages 213-227
pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons....Pages 229-235
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