Ebook: Diagnosis and Identification of Plant Pathogens: Proceedings of the 4th International Symposium of the European Foundation for Plant Pathology, September 9–12, 1996, Bonn, Germany

The diagnosis and identification of plant pathogens provides the basis of plant pathology and phytomedicine. The Executive Committee of the EUROPEAN FOUNDATION FOR PLANT PATHOLOGY (EFPP) had no problem to identify this actual th topic as topic for the 4th Symposium, which was held from September 8 to the 12th at the University of Bonn. It was suggested to have introductory papers and papers on actual research on recently identified topics. The development of diagnosis and pathogen identification is very important to keep plants healthy and to provide a successful and efficient disease control. On the other hand the most important task of the EUROPEAN FOUNDATION FOR PLANT PATHOLOGY is to improve the international communication, especially in the European hemisphere. Another important duty is to provide the contact between all associated societies - of specific importance seems to be the contact to societies and colleagues from eastern European countries. Times have changed and gratefully we are obliged to hold the contact to our colleagues from the east. During the last meeting we could hold this contact to a certain extent and this should be a premise for the future. th During 1998 the EUROPEAN FOUNDATION FOR PLANT PATHOLOGY will join the 7 International Congress of Plant Pathology held at Edinburgh from August 9-14, 1998. th The 5 Symposium of the EUROPEAN FOUNDATION FOR PLANT PATHOLOGY will be arranged by our Italian colleagues.

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The Proceedings of the 4th International Symposium held by the European Foundation for Plant Pathology on Diagnosis and Identification of Plant Pathogens in Bonn, Germany, from September 9th to 12th, 1996, include information on the application of classical as well as novel and innovative methods and techniques. This book gives an overview of the recent status and trends in the diagnosis and identification of viruses, bacteria and fungi in plants. It covers not only the development of methods and application of techniques but also its transformation into practice for disease-forecasting systems.
The book includes 58 oral presentations and 61 poster contributions covering 6 main topics. In addition to introductory overviews of the phytosanitary implications of diagnosis in integrated crop protection, the application of classical and innovative diagnostic methods is stressed. The other contributions cover taxonomy and differentiation of plant pathogens, serological assays, nucleic acid based hybridisation techniques, disease assessment and quantification as well as disease prognosis and expert systems.
This book provides information for all researchers and scientists involved in the production of healthy crop plants. It is of special interest for plant pathologists with responsibilities in the fields of plant quarantine, extension and applied diagnosis of plant pathogens as well as for those working in research and development of innovative crop protection techniques.

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The Proceedings of the 4th International Symposium held by the European Foundation for Plant Pathology on Diagnosis and Identification of Plant Pathogens in Bonn, Germany, from September 9th to 12th, 1996, include information on the application of classical as well as novel and innovative methods and techniques. This book gives an overview of the recent status and trends in the diagnosis and identification of viruses, bacteria and fungi in plants. It covers not only the development of methods and application of techniques but also its transformation into practice for disease-forecasting systems.
The book includes 58 oral presentations and 61 poster contributions covering 6 main topics. In addition to introductory overviews of the phytosanitary implications of diagnosis in integrated crop protection, the application of classical and innovative diagnostic methods is stressed. The other contributions cover taxonomy and differentiation of plant pathogens, serological assays, nucleic acid based hybridisation techniques, disease assessment and quantification as well as disease prognosis and expert systems.
This book provides information for all researchers and scientists involved in the production of healthy crop plants. It is of special interest for plant pathologists with responsibilities in the fields of plant quarantine, extension and applied diagnosis of plant pathogens as well as for those working in research and development of innovative crop protection techniques.
Content:
Front Matter....Pages i-xx
Front Matter....Pages 1-1
The importance of diagnosis and prognosis in integrated plant protection....Pages 3-8
Phytosanitary Implications of Diagnostic Activities and Vice Versa....Pages 9-13
General Aspects on diagnostic Methods: Viruses Microorganisms....Pages 15-16
Fungal Taxonomy in Crisis....Pages 17-21
PCR-Based Diagnostics of Agricultural Pests and Diseases....Pages 23-28
Front Matter....Pages 29-29
Electron microscopic methods currently used for diagnosis and characterization of plant viruses....Pages 31-34
Experiences with the Isolation of Plant Pathogenic Fungi....Pages 35-36
Detection of Different Bacterial and Fungal Pathogens in Apparently Healthy Strawberry Plants....Pages 37-41
The Use of Nested PCR for the Detection of Phytophthora Fragariae in Strawberry....Pages 43-45
rDNA Sequencing: An Essential Guide to the Taxonomy and Diagnosis of Colletotrichum....Pages 47-51
Anastomosis Grouping and Molecular Genetic Typing of Rhizoctonia Solani Causing Crater Disease of Wheat in South Africa....Pages 53-57
Fatty Acid Analysis in the Identification, Taxonomy and Ecology of (Plant Pathogenic) Bacteria....Pages 59-62
Assessment of Microbial Communities by Fatty Acid Analysis....Pages 63-70
Testing Viability and Germination of Plasmodiophora Brassicae (Wor.) Resting Spores Using the Fluorescence Microscope Technique....Pages 71-74
Assessment of Characteristics for Determining Pathogen Populations of Rhizoctonia Solani in Rice-Based Cropping Systems....Pages 75-78
Front Matter....Pages 79-84
Sensitive Detection of Plant Pathogens by Using Immobilized Targets in Tissue Imprinted Membranes....Pages 85-88
Development of a Dot-Immunobinding Assay for the Detection of Phytophthora spp. in Roots....Pages 89-89
Front Matter....Pages 91-94
Detection of Resting Spores of Spongospora Subterranea F.SP. Subterranea Using Serological Techniques....Pages 95-98
Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Verticillium Dahliae Detection in Soil....Pages 99-100
Immunological Methods for the Detection of Verticillium Dahliae in Oilseed Rape....Pages 89-89
Selective Enrichment Improves Isolation, Serological and Molecular Detection of Plant Pathogenic Bacteria....Pages 101-104
Application of Different Techniques to Detect Phytophthora spp. in Roots of Nursery Plants....Pages 105-108
Serological Detection of Spongospora Subterranea F.SP. Subterranea in Soil....Pages 109-112
Prospects of Using PCR for the Detection of Bacterial Pathogens in Potato....Pages 113-116
Development of a Semi-Selective Medium and Use of Serology for Fast and Sensitive Detection of Xanthomonas Campestris PV. Manihotis, Incitant of Bacterial Blight of Cassava....Pages 117-121
Characteristics of Large-Scale Production of Phyto-Sanitary Improved In-Vitro Fruit Tree Cultivars Using In-Vitro Elimination Treatments and Serodiagnostics....Pages 123-124
Production of antibodies against a synthetic peptide based on a sequence common to all tospovirus non-structural NsS protein....Pages 125-128
Front Matter....Pages 129-135
The use of the polymerase chain reaction for the characterisation and diagnosis of yam potyviruses....Pages 137-140
Novel Methods Based on Genomic Amplification for Routine Detection of Potato Leafroll Virus in Potato Tubers....Pages 141-147
Routine Detection of Chrysanthemum Stunt Viroid in Chrysanthemum Plants by Reverse Transcription - Polymerase Chain Reaction....Pages 149-154
IC-RT-PCR Detection of Blackcurrant Reversion Associated Nepovirus, and Perspectives of Its Use for Diagnosis of the Reversion Disease....Pages 155-155
Detection and Identification by PCR-Based Techniques of Diverse Phytoplasmas Infecting Grapevine....Pages 157-160
Diagnosis of Viroids in Fruit Trees: An Italian Experience....Pages 161-167
Detection of Plant Pathogenic Bacteria in Ornamentals by the PCR Technique....Pages 169-172
Differentiation and Detection of the Subspecies of Clavibacter Michiganensis by PCR (Polymerase Chain Reaction) - Techniques....Pages 173-173
Front Matter....Pages 175-178
Molecular diagnosis of rape seed pathogens....Pages 179-182
Identification of Space-Specific Markers of Phytophthora Fragariae with AFLP....Pages 183-187
Physiological Race Determination in Fusarium Oxysporum F.SP. Dianthi by RAPD-PCR....Pages 189-192
Quantitative Estimation of Fusarium Graminearum Using a Novel Solid Phase PCR-Assay....Pages 193-193
Molecular Diagnosis of Toxigenic Fusarium Species....Pages 155-155
Genetic Variability of Ascochyta Rabiei: Population Structure Revealed by RAPD Analysis and Classification into Pathotypes....Pages 195-198
Detection of Lophodermium Seditiosum from Pine Needles Using PCR Techniques....Pages 199-201
Front Matter....Pages 203-206
A Common Basis of Genetically Based and Induced Resistance in Cereals....Pages 207-211
Quantification of Fungal Biomass of a Pathogen and an Antagonist in Necrotic Leaf Tissue Using Fluorescence Microscopy and Image Analysis....Pages 213-217
A Novel Spore Trap for Monitoring Airborne Alternaria Brassicae Spores Using Monoclonal Antibodies....Pages 219-222
Fusarium Ear Blight on Wheat - Epidemiology and Control of Gibberella Zeae, The Teleomorph of Fusarium Graminearum, with Folicur®....Pages 223-226
Risk Assessment Through Quantitative Detection of Soil Suppressiveness Against Plant Diseases Caused by....Pages 227-227
Effect of Crop on the Diversity and the Potential of Fungal Egg Pathogens of Cyst Nematodes....Pages 229-235
Modified Spore Germination Inhibition Technique for Evaluation of Candidate Fungitoxicant (Eucalyptus spp.)....Pages 237-238
Front Matter....Pages 239-242
Introducing Decision Support Systems Available in Germany....Pages 243-246
Estimation of Disease Severity Using Redefined Disease Incidence....Pages 247-251
A Decision Support System for Cercospora Leaf Spot on Sugarbeet....Pages 253-255
An Advisory System for the Control of Brown Rust on Winter Wheat in Northern Italy....Pages 257-263
Causal Agents of Hop-Dying in Spalt....Pages 265-265
Front Matter....Pages 267-270
Investigation of Claviceps Purpurea /FR./ Tul in the Poacea Family Plants in Lithuania....Pages 271-274
First Report on Anthracnose Diagnosis and Colletotrichum spp. Identification in White Lupin (Lupinus Albus L.) Infection in Poland....Pages 275-279
Suitability of Electron Microscopy for Routine Diagnosis of Plant Virus Diseases in Extension Service....Pages 281-284
Use of the Scanning Electron Microscope in the Identification of Powdery Mildews....Pages 287-289
Analysis of Pyrenophora Teres (Died.) Drechs. Population from Slovakia....Pages 291-293
Detection of Plum Pox Virus by Different Methods....Pages 265-265
Image Analysis and Archiving as an Aid to Fungal Identification....Pages 295-297
Anastomosis Groups and Pathogenicity of Rhizoctonia Solani Isolates From Tobacco in Italy....Pages 299-301
Use of Fungicide-Resistance for the Characterisation and Differentiation of Venturia Inaequalis Strains - Suitability for Competitivity Studies....Pages 303-306
Isolation of Micromycetes from Pea Seeds....Pages 307-309
Isolation and Identification of Fusarium Spp. and Microdochium Nivale from Winter Wheat....Pages 311-313
Quantification of Phytophthora Infestans During Early Stages of Pathogenesis....Pages 315-316
Description of Microbial Communities of Rhizosphere Soil Based on the Phospholipid Fatty - Acid Spectrum Analysis of Microorganisms....Pages 317-319
Scanning Electron Microscopy of Verticillium Dahliae on the Cotton Root Surface....Pages 321-323
Isolation and Identification of Arbuscular Mycorrhizal Fungi (AMF)....Pages 325-327
Analysis of AMF-Glomus Caledonium J 55-Host Interaction — Activity, Isozyme Patterns and Plant Response....Pages 329-332
Antagonism Between Mycorrhizal Fungi and Heterobasidion Annosum (Fr.) Bref. - Scanning Electron Microscopical Studies....Pages 333-336
Pathogenicity of the Isolates of Rhizoctonia Cerealis and R. Solani from the Poznan Region Towards the Wheat Seedlings....Pages 337-340
The Presence of Spongospora Subterranea F.SP. Subterranea in the Northern Areas of Pakistan Confirmed by Microscopy, Serology and Bioassay....Pages 341-343
Front Matter....Pages 345-348
Differentiation and Immunochemical Characterisation of Various Potato Viruses and their Strains by Means of Monoclonal Antibodies....Pages 349-351
Insight® - A New Diagnostic Tool for Mycosphaerella spp. in Banana Leaves....Pages 353-356
Feasibility of a Diagnostic Kit for Multiplex DIG-Chemiluminescent Detection of Tomato Viruses....Pages 357-360
Development of Monoclonal Antibodies for the Detection and Identification of Monilinia spp. Causing Brown Rot of Stone and Pome Fruit....Pages 361-363
Determination of Clavibacter Michiganensis subsp. Insidiosus Infection in Lucerne Seeds by Immunofluorescence Staining and Direct Isolation Using Selective Medium....Pages 365-367
Detection of Ryegrass Mosaic Virus by Monoclonal Antibodies and Immunocapture PCR....Pages 371-373
Detection of Poplar Mosaic Carlavirus (POPMV) by Immuno-Capture RT-PCR....Pages 265-265
Indirect Immunofluorescence - an Useful Method in Studies on Some Fungal Pathogens....Pages 375-376
The Use of Physiological and Biochemical Parameters of Plants for the Estimation of the Resistance to Stem Rust....Pages 377-383
Detection and differentiation of Three potyviruses infecting sweet potato by PCR....Pages 385-389
Differentiation of Fusarium Species by Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Ribosomal DNA....Pages 391-393
Diagnosis and Identification of Pathogens Causing Apple Storage Rots, by Using PCR-RFLP and Allele Specific Amplification....Pages 395-397
Isolation and Characterization of a Nepo-Type Virus from Reverted Blackcurrant (Ribes nigrum L.)....Pages 399-402
Differential Detection of Erwinia Amylovora Isolates from Fruit Trees and Rubus spp. Based on Ribosomal DNA and PCR....Pages 403-405
Application of Polymerase Chain Reaction (PCR) for the Positive Identification of Two Phytoplasma Diseases in Poland....Pages 407-409
Silicacapture-Reverse Transcription-Polymerase Chain Reaction (SC-RT-PCR): Application for the Detection of Several Plant Viruses....Pages 411-413
Non-Radioactive Probe for the Detection of Strawberry Vein Banding Virus....Pages 417-419
Detection of Bacterial Diseases of Tomatoes by the Polymerase Chain Reaction (PCR) and Serology....Pages 421-424
Detection of Agrobacterium tumefaciens in Soil Using PCR....Pages 425-428
Front Matter....Pages 429-432
Sequence Analysis of the Ribosomal DNA Spacer Region of Pyrenophora spp.; Evaluation of its Potential for PCR Detection in a Seed Health Test....Pages 433-435
Differentiation of Allium Potyviruses by Polymerase Chain Reaction....Pages 437-439
Detection and Differentiation of Sweet Potato Closteroviruses by RT-PCR and Single-Strand Conformation Polymorphism....Pages 441-443
The Use of Video Time-Lapse Recordings to Study the Effects of Antifungal Compounds on Phytopathogenic Fungi....Pages 445-448
Identification and Quantitative Determination of Phytophthora Species Infecting Ornamental Plants in Nurseries....Pages 449-451
Evaluation of Three Methods for the Detection of Septoria Spp. in Wheat Leaves....Pages 453-455
Variation for Resistance to Fusarium Head Blight (Fusarium Culmorum) in Somaclones of Winter Triticale....Pages 457-460
Quantification of Phytophthora Nicotianae on Tomatos Grown with The Nutrient Film Technique (NFT)....Pages 265-265
Comparison of Willow Leaf Rust Quantification Methods....Pages 461-463
Comparison Between a DNA-Based Assay and a Soil Bioassay in Quantifying the Amount of Gaeumannomyces Graminis Var. Tritici in Soil....Pages 465-467
Identification of Complex Pathotypes and Seasonal Changes of their Frequency in Subregional Populations of Barley Powdery Mildew (Erysiphe Graminis F.SP. Hordei)....Pages 469-471
Characterization and Role in the Pathogenesis of Potatoes of a Novel Pectate Lyase from Eriwinia Chrysanthemi 3937....Pages 473-475
Fluorescent Parameters of Two Cucumber Cultivars Differing in Resistance to Mildew....Pages 479-481
The Rate Constants of Electron Transport in RC PS2 During Disease Development (Stem Rust, Powdery Mildew)....Pages 483-485
Rapid Assessment of Aggressiveness of Root Rot Agents of Alfalfa (Medicago L.)....Pages 487-489
The Role of Prunus Spinosa L. in Epidemiology of Plum Pox Virus in the Czech Republic....Pages 491-494
Yield Reduction and Seed Transmission Rate of Lithuanian Isolate of Broad Bean True Mosaic Virus....Pages 495-497
Characterization of Fusarium Oxysporum Populations by Growth Parameter Evaluation in Microtiter Plates....Pages 499-501
Study on the Influence of Ascochyta Fabae and Botrytis Fabae on Two Growing types of Faba Bean....Pages 503-505
Back Matter....Pages 507-509
....Pages 511-514