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Electrical Manipulation of Cells provides an authoritative and up-to-date review of the field, covering all the major techniques in a single source. The book features broad coverage that ranges from the mechanisms of action of external electrical fields on biological material to the ways in which electrical stimuli are employed to manipulate cells. Bringing together the work of leading international authorities, the book covers membrane breakdown, gene delivery, electroporation, electrostimulation, cell movement, hybridoma production, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation.
For each topic, the authors discuss the relevance of the approach to the current state of the art of biotechnology. Electrical Manipulationof Cells is an unmatched source of information for anyone involved in the manipulation of cells, particularly biotechnologists, cell biology, microbiologists, biophysicists and plant scientists. For researchers, the book provides technical material that ccan be employed in their own work. Students will gain thorough appreciation of the applications of this important technique.




Electrical Manipulation of Cells provides an authoritative and up-to-date review of the field, covering all the major techniques in a single source. The book features broad coverage that ranges from the mechanisms of action of external electrical fields on biological material to the ways in which electrical stimuli are employed to manipulate cells. Bringing together the work of leading international authorities, the book covers membrane breakdown, gene delivery, electroporation, electrostimulation, cell movement, hybridoma production, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation.
For each topic, the authors discuss the relevance of the approach to the current state of the art of biotechnology. Electrical Manipulationof Cells is an unmatched source of information for anyone involved in the manipulation of cells, particularly biotechnologists, cell biology, microbiologists, biophysicists and plant scientists. For researchers, the book provides technical material that ccan be employed in their own work. Students will gain thorough appreciation of the applications of this important technique.


Electrical Manipulation of Cells provides an authoritative and up-to-date review of the field, covering all the major techniques in a single source. The book features broad coverage that ranges from the mechanisms of action of external electrical fields on biological material to the ways in which electrical stimuli are employed to manipulate cells. Bringing together the work of leading international authorities, the book covers membrane breakdown, gene delivery, electroporation, electrostimulation, cell movement, hybridoma production, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation.
For each topic, the authors discuss the relevance of the approach to the current state of the art of biotechnology. Electrical Manipulationof Cells is an unmatched source of information for anyone involved in the manipulation of cells, particularly biotechnologists, cell biology, microbiologists, biophysicists and plant scientists. For researchers, the book provides technical material that ccan be employed in their own work. Students will gain thorough appreciation of the applications of this important technique.
Content:
Front Matter....Pages i-xii
Electrofusion and Electroporation Equipment....Pages 1-14
Electrically Stimulated Membrane Breakdown....Pages 15-36
Cell Electrorotation....Pages 37-70
Dielectrophoresis: Effect of Nonuniform Electrical Fields on Cell Movement....Pages 71-99
Electrofusion of Animal Cells....Pages 101-117
Cell-Tissue Electrofusion....Pages 119-137
Electrofusion of Plant Protoplasts for Hybrid Plant Production....Pages 139-156
Gene Delivery by Membrane Electroporation....Pages 157-183
Clinical Applications of Electroporation....Pages 185-199
Electroporation and Transgenic Plant Production....Pages 201-222
Electromagnetic Cell Stimulation....Pages 223-247
Electrostimulation of Cells by Weak Electric Currents....Pages 249-272
Stimulation of Plant Cell Division and Organogenesis by Short-Term, High-Voltage Electrical Pulses....Pages 273-286
Back Matter....Pages 287-292


Electrical Manipulation of Cells provides an authoritative and up-to-date review of the field, covering all the major techniques in a single source. The book features broad coverage that ranges from the mechanisms of action of external electrical fields on biological material to the ways in which electrical stimuli are employed to manipulate cells. Bringing together the work of leading international authorities, the book covers membrane breakdown, gene delivery, electroporation, electrostimulation, cell movement, hybridoma production, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation.
For each topic, the authors discuss the relevance of the approach to the current state of the art of biotechnology. Electrical Manipulationof Cells is an unmatched source of information for anyone involved in the manipulation of cells, particularly biotechnologists, cell biology, microbiologists, biophysicists and plant scientists. For researchers, the book provides technical material that ccan be employed in their own work. Students will gain thorough appreciation of the applications of this important technique.
Content:
Front Matter....Pages i-xii
Electrofusion and Electroporation Equipment....Pages 1-14
Electrically Stimulated Membrane Breakdown....Pages 15-36
Cell Electrorotation....Pages 37-70
Dielectrophoresis: Effect of Nonuniform Electrical Fields on Cell Movement....Pages 71-99
Electrofusion of Animal Cells....Pages 101-117
Cell-Tissue Electrofusion....Pages 119-137
Electrofusion of Plant Protoplasts for Hybrid Plant Production....Pages 139-156
Gene Delivery by Membrane Electroporation....Pages 157-183
Clinical Applications of Electroporation....Pages 185-199
Electroporation and Transgenic Plant Production....Pages 201-222
Electromagnetic Cell Stimulation....Pages 223-247
Electrostimulation of Cells by Weak Electric Currents....Pages 249-272
Stimulation of Plant Cell Division and Organogenesis by Short-Term, High-Voltage Electrical Pulses....Pages 273-286
Back Matter....Pages 287-292
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