Ebook: Analytical Use of Fluorescent Probes in Oncology

Fluorescence is a very powerful tool for work at the frontier of cell biology, photobiology and bioinstrumentation. The stated aim of the workshop was to highlight the significance of fluorescence work for the understanding of cell and tissue physiology, physiopathology and pharmacology, particulary in terms of the analytical use of fluorescent probes in oncology. In the organization of the workshop a multidisciplinary approach was selected. The purpose of the Advanced Research Workshop (ARW) was to bring together researchers in the various disciplines of tissue optics, imaging, microspectrofluorometry and state of the art probes, in order to explore the full benefits that can be derived in biomedicine through the convergence of these approaches. When applied to in vivo and in situ studies, fluorescence and related optical methods enable us to explore within tissues, cells and organelles photon effects previously understood only in solution photochemistry. Processes which can be studied at the molecular level by photophysics, photochemistry and physical chemistry can be evaluated in living tissue by fluorescence spectroscopy and imaging at the intracellular level in terms of structure and function. Thus, fluorescence adds a new dimension to cell biology and physiology. This approach is now supported by a full and versatile, rapidly growing armamentarium of new selective probes for organelles, enzymes, cations, cytoskeleton and metabolic control.

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In Vivo Spectroscopy and Imaging: Fluorescence: Lifetime-based Sensing in Tissues and Other Random Media with Measurement of Photon Migration; E. Sevich-Muraca.Multidrug Resistance: Multidrug Resistance and Flow Cytometry: Going beyond Pglycoprotein; D. Hedley.Apoptosis: The Detection of Apoptotic Cells and Their Quantification by Flow Cytometry. Adhesion: Prognostic and Biological Implications of CD44 Isoform Expression in Aggressive NonHodgkin's Lymphoma; M. Shipp.Cyclins/Cell Cycle: Cancer: A Disease of the Cell Cycle; K. Keyomarsi.Fluorescence Imaging and Immunofluorescence: Localization and Selectivity of Anthracyclines in Cardiac Muscle Cells: Relevance of Charge and Lipophilicity to Multidrug Resistance; T.J. Lampidis.Fluorescence Probing of Cytoskeleton: Probing of Microtubule Structures with Monoclonal Antibodies and Immunofluorescence Microscopy; P. Draber.Fluorescence and Gene Expression: Cell Expression System in V79 Cells Transfected with cyt P450 and Its Performance in Metabolism of Drugs; J. Doehmer.Metabolic ControlMechanisms in Living Cells: Fluorescence Probes. New Methods. Cytoskeleton Probes. Fluorescence Spectroscopy. Photochemotherapy. Poster Presentations. 43 additional articles. Index.

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In Vivo Spectroscopy and Imaging: Fluorescence: Lifetime-based Sensing in Tissues and Other Random Media with Measurement of Photon Migration; E. Sevich-Muraca.Multidrug Resistance: Multidrug Resistance and Flow Cytometry: Going beyond Pglycoprotein; D. Hedley.Apoptosis: The Detection of Apoptotic Cells and Their Quantification by Flow Cytometry. Adhesion: Prognostic and Biological Implications of CD44 Isoform Expression in Aggressive NonHodgkin's Lymphoma; M. Shipp.Cyclins/Cell Cycle: Cancer: A Disease of the Cell Cycle; K. Keyomarsi.Fluorescence Imaging and Immunofluorescence: Localization and Selectivity of Anthracyclines in Cardiac Muscle Cells: Relevance of Charge and Lipophilicity to Multidrug Resistance; T.J. Lampidis.Fluorescence Probing of Cytoskeleton: Probing of Microtubule Structures with Monoclonal Antibodies and Immunofluorescence Microscopy; P. Draber.Fluorescence and Gene Expression: Cell Expression System in V79 Cells Transfected with cyt P450 and Its Performance in Metabolism of Drugs; J. Doehmer.Metabolic ControlMechanisms in Living Cells: Fluorescence Probes. New Methods. Cytoskeleton Probes. Fluorescence Spectroscopy. Photochemotherapy. Poster Presentations. 43 additional articles. Index.
Content:
Front Matter....Pages i-x
Overview....Pages 1-23
Detection of Breast Tumors by Absorption and Fluorescence of Photon Diffusive Waves....Pages 25-30
Fluorescence-Lifetime Based Sensing in Tissues and Other Random Media with Measurements of Photon Migration....Pages 31-40
Spectroscopy and Tomography of Tissues in the Frequency-Domain....Pages 41-52
Fret Microscopy in Cellular Signal Transduction....Pages 53-57
Photoacoustic Spectroscopy Applied to Biological Materials....Pages 59-64
Recent Developments in Fluorescence Spectroscopy....Pages 65-79
Photonic Pathology....Pages 81-89
Laser Scanning Confocal Fluorescence Microscopy of Cell Function....Pages 91-96
Sub-Cellular Localization of Second Generation PDT Photosensitizers Studied by Microspectrofluorometry....Pages 97-111
Photofrin Uptake as a Function of Tumor Size and the Detection of Microscopic Nests of Tumor Cells by Photofrin Fluorescence....Pages 113-121
The Detection of Apoptotic Cells and Their Quantification by Flow Cytometry....Pages 123-128
Adhesion Stabilization of Blood Borne Cancer Cells in the Microcirculation....Pages 129-135
Probing of Connexin Channels....Pages 137-148
The Analysis of Drug-Induced Cell Cycle Perturbations by Flow Cytometry....Pages 149-156
The Effects of Tumor Heterogeneity on the Flow Cytometric Analysis of Clinical Specimens....Pages 157-172
Videomicrofluorometry of Progesterone Receptors and Their Genes in Breast Cancer Cells....Pages 173-179
Immunophotodetection of Cancer by Antibody-Indocyanin Conjugates....Pages 181-188
Three-Dimensional Optical Functional Imaging of Tissue with Two-Photon Excitation Laser Scanning Microscopy....Pages 189-204
Estrogen Receptor Imaging Using Intrinsically Fluorescent Ligands....Pages 205-211
Videomicrofluorometry and Intracellular Ionic Concentration in Single Living Cells....Pages 213-219
Multiwavelength MicroFluorometry to Investigate Cytotoxic Processes on Sensitive and Resistant Human Lymphoblastoid CCRF-Cem Lines....Pages 221-229
Laser-Induced Auto Fluorescence of Normal and Tumor Bladder Cells and Tissues....Pages 231-239
Fluorescent Imaging of Membrane Potential Components....Pages 241-247
Probing of Microtubule Structures with Monoclonal Antibodies and Immunofluorescence Microscopy....Pages 249-256
Immunofluorescence Detection of Cytoskeletal Structures....Pages 257-264
Cell Expression System in V79 Cells Transfected with Cytochrome P450 and its Performance in Metabolism Studies....Pages 265-271
Direct Transfer of Control and Multidrug Resistance....Pages 273-282
Instrumentation Design for Study of Metabolic Control in Living Cells....Pages 283-292
Use of Fluorescent Derivatives for Labeling Cells; Diagnosing, Preventing, and Correcting Genetic Diseases; Photosynthesizing and Killing of Tumors, Parasites, and Viruses....Pages 293-298
Ca2+ Signaling and Cytoskeleton Reorganization in Endothelial Cells Stimulated by Bradykinin....Pages 299-308
Integration of Optical Techniques in Scanning Probe Microscopes....Pages 309-315
Perfusion, Temperature, and PO2 Control in a Leiden Culture Bath for Single Cell Measurements and Manipulations....Pages 317-323
Immunofluorescence Study of Intermediate Filament Protein Expression under Normal and Pathological Conditions....Pages 325-330
The Use of Fluorescent Probes in Characterizing Drug Resistance in Cultured Cells....Pages 331-337
A High Resolution Multimode Digital Imaging System for Fluorescence Studies of Mitosis....Pages 339-347
Use of Photodiode Array Fluorescence Spectroscopy in Biochemistry, Pharmacology, and Oncology....Pages 349-356
Hydrophobic Photosensitizers Delivery to Tumor Tissues by an Amphiphilic Peptide....Pages 357-362
Merocyanine 540—A Fluorescent Dye and a Biological Probe....Pages 363-370
High Pressure Infrared Spectroscopy in Biological and Biomedical Studies....Pages 371-382
Fluorescence Spectroscopy for Diagnosis of Cancerous Tissues....Pages 383-392
Solvent-Dependent Excited State Properties of Psoralens....Pages 393-404
Fluorescent Probes in Breast Tumor....Pages 405-409
Use of Fluorescence in Situ Hybridization for Mapping and Ordering YAC Clones from Chromosomal Regions with Suspected Tumor-Suppressor Activity....Pages 411-417
Photochemical and Photophysical Studies of an Anthocyanin....Pages 419-421
Selectivity and Localization of Anthracyclines in Cardiac-Muscle and Non-Muscle Cells Relevance to Multidrug Resistance....Pages 423-424
Back Matter....Pages 425-426
....Pages 427-448