Ebook: Epithelial Transport: A guide to methods and experimental analysis

Building from general principles, the authors clearly explain the fundamental role of epithelia in plasma electrolyte and water balance. Emphasis is placed on experimental approaches and methodology. A comprehensive glossary of terms is included.

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Building from general principles, the authors clearly explain the fundamental role of epithelia in plasma electrolyte and water balance. Emphasis is placed on experimental approaches and methodology. A comprehensive glossary of terms is included.

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Building from general principles, the authors clearly explain the fundamental role of epithelia in plasma electrolyte and water balance. Emphasis is placed on experimental approaches and methodology. A comprehensive glossary of terms is included.
Content:
Front Matter....Pages i-xi
Epithelial structure and function....Pages 1-20
Epithelial transport proteins....Pages 21-48
Epithelial polarity....Pages 49-69
Characterization of epithelial ion transport....Pages 70-92
Epithelial electrophysiology....Pages 93-117
Impedance analysis of epithelia....Pages 118-145
Measurements and interpretation of cytoplasmic ion activities....Pages 146-166
Measurement of epithelial cell volume....Pages 167-189
Methods and experimental analysis of isolated epithelial cell membranes....Pages 190-211
Methods and experimental analysis of single ion channels....Pages 212-235
Epithelial cell culture....Pages 236-255
Signaling pathways regulating ion transport in polarized cells....Pages 256-274
The cytoskeleton and epithelial function....Pages 275-305
Future perspectives: molecular biology and pathophysiology....Pages 306-322
Back Matter....Pages 323-355

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Building from general principles, the authors clearly explain the fundamental role of epithelia in plasma electrolyte and water balance. Emphasis is placed on experimental approaches and methodology. A comprehensive glossary of terms is included.
Content:
Front Matter....Pages i-xi
Epithelial structure and function....Pages 1-20
Epithelial transport proteins....Pages 21-48
Epithelial polarity....Pages 49-69
Characterization of epithelial ion transport....Pages 70-92
Epithelial electrophysiology....Pages 93-117
Impedance analysis of epithelia....Pages 118-145
Measurements and interpretation of cytoplasmic ion activities....Pages 146-166
Measurement of epithelial cell volume....Pages 167-189
Methods and experimental analysis of isolated epithelial cell membranes....Pages 190-211
Methods and experimental analysis of single ion channels....Pages 212-235
Epithelial cell culture....Pages 236-255
Signaling pathways regulating ion transport in polarized cells....Pages 256-274
The cytoskeleton and epithelial function....Pages 275-305
Future perspectives: molecular biology and pathophysiology....Pages 306-322
Back Matter....Pages 323-355
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