Ebook: Gene Technology: Stem Cell and Leukemia Research

The 11 th meeting in "Modern Trends in Human Leukemia" took place from June 19 to 21, 1994 in Wilsede in the middle of the Liineburger Heide, South of Hamburg. Interwoven with the Leukemia program was the Nato-sponsored Symposium of the ASI-Series "Gene Technology in Analysis of Malignant and Inherited Human Diseases Related to Development" . The Wilsede meeting was continued on a ship of the Neva leading through lake Ladoga and lake Onega. The topics of both meetings included discussion on recent progress isolation and development of hematopoietic stem cells, genes crucial for development and diseases, methods of gene transfer, application of gene transfer; oncogenes and anti-oncogenes as targets for gene therapy; receptors and their ligands in normal development and diseases, immunology and immunotherapy, radiation biology, clinical leukemias and bone marrow transplantation. The Nato workshop concentrated not only on analysis of cell systems useful for somatic gene therapy, but also on actual themes directly related to correction of human diseases. The latter aspects emphasized themes related to biotechnology, the first part was by nature more general. We also included a few contributions that discussed perspectives for the future of gene therapy and possible relationships to evolution.

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The importance of gene therapy is increasing more and more. This book covers both somatic gene therapy of inherited human diseases as well as of cancer, with emphasis on malignancies of the hematopoietic system.
Topics include the isolation and development of hematopoietic stem cells, genes involved in development and diseases, methods of gene transfer, carrying out gene transfer, oncogenes and antioncogenes as targets for gene therapy, receptors and their ligands in normal development and diseases, immunology and immunotherapy, radiation biology, clinical leukemias and bone marrow transplantation.

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The importance of gene therapy is increasing more and more. This book covers both somatic gene therapy of inherited human diseases as well as of cancer, with emphasis on malignancies of the hematopoietic system.
Topics include the isolation and development of hematopoietic stem cells, genes involved in development and diseases, methods of gene transfer, carrying out gene transfer, oncogenes and antioncogenes as targets for gene therapy, receptors and their ligands in normal development and diseases, immunology and immunotherapy, radiation biology, clinical leukemias and bone marrow transplantation.
Content:
Front Matter....Pages I-XII
Front Matter....Pages 1-1
Hematopoietic Differentiation of Embryonal Stem Cells in vitro.....Pages 3-10
Post-irradiated canine serum (PICS-J) stimulates megakaryocyte (MK) progenitors and thrombopoiesis in-vitro....Pages 11-27
The Adhesion Molecules, PECAM-1, ICAM-3 and CD33, on CD34+ Haematopoietic Progenitor Cells....Pages 29-76
Hematopoietic stem cell: developmental potential and proliferative activity....Pages 77-90
The Effect of Methylprednisolone on Bone Marrow and Spleen Clonogenic Fibroblasts in Humans....Pages 91-97
Umbilical cord blood: Morphological composition and functional characteristics....Pages 99-106
Stroma cell dependent leukemias Growth of PGM-1 leukemia cells on stromal layers....Pages 107-116
Front Matter....Pages 117-117
The Role of Non-receptor Protein Tyrosine Kinases in Receptor Mediated Cellular Activation and Immunodeficiency Disease....Pages 119-134
Directed Gene Trapping in Embryonic Stem Cells....Pages 135-144
Molecular Basis of Genetic Disorders of Pigmentation in Humans and Mice....Pages 145-174
MDR-1 Gene Expression in Chronic Myelogenous Leukemia: Prognostic Marker of the Disease....Pages 175-179
Front Matter....Pages 181-181
Towards Gene Therapy for Haemophilia B....Pages 183-188
Inhibition of autocrine TGF-? in CD34+ human progenitor cells reveals their potentiality to engraft adults and improves gene transfer efficiency....Pages 189-192
Gene Transfer into Hemopoietic Progenitors: Implications for Bone Marrow Transplantation....Pages 193-202
Erythropoietin and the ENV gp55 of the Spleen Focus Forming Virus (SFFV) Interact Differently with Erythroid Cells in the Mouse and in Tissue Culture....Pages 203-218
Contribution of the Retroviral Leader to Gene Transfer and Expression in Packaging Cells and Myeloid Stem and Progenitor Cells....Pages 219-228
Site-directed Mutagenesis of the cGMP Phosphodiesterase Inhibitory g Subunit from Bovine Rods: New Mechanisms of Catalytic Subunits Inhibition and Activation....Pages 229-244
Front Matter....Pages 245-245
Genetic Approaches to Defining Signaling by the CML-Associated Tyrosine kinase BCR-ABL1....Pages 247-262
Analysis of Oncosuppressor Gene Function in the Mouse by Gene Targeting....Pages 263-281
Front Matter....Pages 283-283
Cloning of retroviral insertion sites possibly involved in growth-factor autonomy....Pages 285-293
Front Matter....Pages 283-283
Function of the IL-3 receptor system in hematopoiesis....Pages 295-307
Altered Growth Factor Response in Myeloid Progenitor Cell Mutants Derived after Retroviral Insertlonal Mutagenesis....Pages 307-319
Signaling by the Erythropoietin receptor in retrovirally transduced normal hematopoietic progenitors: A conserved proline-rich motif is required for association with JAK2....Pages 321-331
Spontaneous Knockout of CSF-1 Gene in the Mouse as a Model to Study the Organization of the Macrophage System....Pages 333-350
Front Matter....Pages 351-351
Lifting a tip of the veil of human minor Histocompatibility antigens....Pages 353-359
Immune escape through T cell antagonism?....Pages 361-368
The privilege of expressing CD44v - used by immune cells, misused by tumour cells....Pages 369-379
Transplantation Immunology: Peptides in the groove cause histocompatibility....Pages 381-396
Front Matter....Pages 397-397
Coupling of Membrane Pathology and DNA Damage in Irradiated Lymphoid Cell Population....Pages 399-404
Effects of Radiation on the Plasma Membrane and Expression of Membrane Bound Hematopoietic Cytokines....Pages 405-418
Front Matter....Pages 419-419
Prognostic Value of Day 8 Peripheral Blood Responce for Children with Acute Lymphocytic Leukemia....Pages 421-428
GM-CSF in Addition to Chemotherapy of ALL for Kinetics Based Protection of Stem Cells and Stimulation of Haemopoiesis.A randomized Study....Pages 429-440
Cytogenetic Abnormalities and Their Clinical and Prognosti Value in Acute Leukemia....Pages 441-447
Production of Interleukin-2 and Natural Killer (NK) Activity in Patients with Multiple Myeloma....Pages 449-453
Multiple Myeloma Associated with Amyloidosis....Pages 455-460
Distribution of HLA-DRB1 Alleles in Patients with Chronic Myelogenous Leukemia Differs from That in Healthy Donors....Pages 461-465
The Relationship of MDR-1 Gene Expression and Chemotherapy Regimens in Acute Leukemia....Pages 467-473
Immunophenotyping of blast crisis during the chronic myelogenous leukemia....Pages 475-484
The treatment of acute lymphoblastic leukemia in children according to BFM protocols....Pages 485-491
Front Matter....Pages 493-493
Carcinoembryonic antigen (CEA) in blood serum of patients with bone marrow transplantation....Pages 495-498
Front Matter....Pages 493-493
Cytogenetic Monitoring in The Post BMT Period....Pages 499-504
First Experience in the Field of Bone Marrow Transplantation at the St.-Peterburg BMT Center....Pages 505-511
Immunoglobulin E Levels in Allogenic BMT....Pages 513-517
Prognostic Factors and Indications for Haemopoietic Stem Cells Transplantation in Patients with Multiple Myeloma....Pages 519-529
Mobilization of Progenitor Cells for Autologous and Allogeneic Transplantation....Pages 531-536
Transfusion problems of allogenic bone marrow transplantation (BMT)....Pages 537-540
Back Matter....Pages 541-546

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The importance of gene therapy is increasing more and more. This book covers both somatic gene therapy of inherited human diseases as well as of cancer, with emphasis on malignancies of the hematopoietic system.
Topics include the isolation and development of hematopoietic stem cells, genes involved in development and diseases, methods of gene transfer, carrying out gene transfer, oncogenes and antioncogenes as targets for gene therapy, receptors and their ligands in normal development and diseases, immunology and immunotherapy, radiation biology, clinical leukemias and bone marrow transplantation.
Content:
Front Matter....Pages I-XII
Front Matter....Pages 1-1
Hematopoietic Differentiation of Embryonal Stem Cells in vitro.....Pages 3-10
Post-irradiated canine serum (PICS-J) stimulates megakaryocyte (MK) progenitors and thrombopoiesis in-vitro....Pages 11-27
The Adhesion Molecules, PECAM-1, ICAM-3 and CD33, on CD34+ Haematopoietic Progenitor Cells....Pages 29-76
Hematopoietic stem cell: developmental potential and proliferative activity....Pages 77-90
The Effect of Methylprednisolone on Bone Marrow and Spleen Clonogenic Fibroblasts in Humans....Pages 91-97
Umbilical cord blood: Morphological composition and functional characteristics....Pages 99-106
Stroma cell dependent leukemias Growth of PGM-1 leukemia cells on stromal layers....Pages 107-116
Front Matter....Pages 117-117
The Role of Non-receptor Protein Tyrosine Kinases in Receptor Mediated Cellular Activation and Immunodeficiency Disease....Pages 119-134
Directed Gene Trapping in Embryonic Stem Cells....Pages 135-144
Molecular Basis of Genetic Disorders of Pigmentation in Humans and Mice....Pages 145-174
MDR-1 Gene Expression in Chronic Myelogenous Leukemia: Prognostic Marker of the Disease....Pages 175-179
Front Matter....Pages 181-181
Towards Gene Therapy for Haemophilia B....Pages 183-188
Inhibition of autocrine TGF-? in CD34+ human progenitor cells reveals their potentiality to engraft adults and improves gene transfer efficiency....Pages 189-192
Gene Transfer into Hemopoietic Progenitors: Implications for Bone Marrow Transplantation....Pages 193-202
Erythropoietin and the ENV gp55 of the Spleen Focus Forming Virus (SFFV) Interact Differently with Erythroid Cells in the Mouse and in Tissue Culture....Pages 203-218
Contribution of the Retroviral Leader to Gene Transfer and Expression in Packaging Cells and Myeloid Stem and Progenitor Cells....Pages 219-228
Site-directed Mutagenesis of the cGMP Phosphodiesterase Inhibitory g Subunit from Bovine Rods: New Mechanisms of Catalytic Subunits Inhibition and Activation....Pages 229-244
Front Matter....Pages 245-245
Genetic Approaches to Defining Signaling by the CML-Associated Tyrosine kinase BCR-ABL1....Pages 247-262
Analysis of Oncosuppressor Gene Function in the Mouse by Gene Targeting....Pages 263-281
Front Matter....Pages 283-283
Cloning of retroviral insertion sites possibly involved in growth-factor autonomy....Pages 285-293
Front Matter....Pages 283-283
Function of the IL-3 receptor system in hematopoiesis....Pages 295-307
Altered Growth Factor Response in Myeloid Progenitor Cell Mutants Derived after Retroviral Insertlonal Mutagenesis....Pages 307-319
Signaling by the Erythropoietin receptor in retrovirally transduced normal hematopoietic progenitors: A conserved proline-rich motif is required for association with JAK2....Pages 321-331
Spontaneous Knockout of CSF-1 Gene in the Mouse as a Model to Study the Organization of the Macrophage System....Pages 333-350
Front Matter....Pages 351-351
Lifting a tip of the veil of human minor Histocompatibility antigens....Pages 353-359
Immune escape through T cell antagonism?....Pages 361-368
The privilege of expressing CD44v - used by immune cells, misused by tumour cells....Pages 369-379
Transplantation Immunology: Peptides in the groove cause histocompatibility....Pages 381-396
Front Matter....Pages 397-397
Coupling of Membrane Pathology and DNA Damage in Irradiated Lymphoid Cell Population....Pages 399-404
Effects of Radiation on the Plasma Membrane and Expression of Membrane Bound Hematopoietic Cytokines....Pages 405-418
Front Matter....Pages 419-419
Prognostic Value of Day 8 Peripheral Blood Responce for Children with Acute Lymphocytic Leukemia....Pages 421-428
GM-CSF in Addition to Chemotherapy of ALL for Kinetics Based Protection of Stem Cells and Stimulation of Haemopoiesis.A randomized Study....Pages 429-440
Cytogenetic Abnormalities and Their Clinical and Prognosti Value in Acute Leukemia....Pages 441-447
Production of Interleukin-2 and Natural Killer (NK) Activity in Patients with Multiple Myeloma....Pages 449-453
Multiple Myeloma Associated with Amyloidosis....Pages 455-460
Distribution of HLA-DRB1 Alleles in Patients with Chronic Myelogenous Leukemia Differs from That in Healthy Donors....Pages 461-465
The Relationship of MDR-1 Gene Expression and Chemotherapy Regimens in Acute Leukemia....Pages 467-473
Immunophenotyping of blast crisis during the chronic myelogenous leukemia....Pages 475-484
The treatment of acute lymphoblastic leukemia in children according to BFM protocols....Pages 485-491
Front Matter....Pages 493-493
Carcinoembryonic antigen (CEA) in blood serum of patients with bone marrow transplantation....Pages 495-498
Front Matter....Pages 493-493
Cytogenetic Monitoring in The Post BMT Period....Pages 499-504
First Experience in the Field of Bone Marrow Transplantation at the St.-Peterburg BMT Center....Pages 505-511
Immunoglobulin E Levels in Allogenic BMT....Pages 513-517
Prognostic Factors and Indications for Haemopoietic Stem Cells Transplantation in Patients with Multiple Myeloma....Pages 519-529
Mobilization of Progenitor Cells for Autologous and Allogeneic Transplantation....Pages 531-536
Transfusion problems of allogenic bone marrow transplantation (BMT)....Pages 537-540
Back Matter....Pages 541-546
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