Ebook: Cell Culture Engineering IV: Improvements of Human Health

Cell Culture Engineering IV, Improvements of Human Health covers the latest approaches to improving the cell host through improved understanding of the molecular biology, the development of novel vaccines, approaches to bioreactor design and operation, monitoring techniques in process control and quality related topics. The work was carefully put together as one result of the Cell Culture Engineering IV Meeting held in San Diego, U.S.A. in 1994, however, the book may not be perceived as a proceedings volume - the criteria of the book series apply.
For cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment, as well as in (biotechnology or pharmaceutical) industry.

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Cell Culture Engineering IV, Improvements of Human Health covers the latest approaches to improving the cell host through improved understanding of the molecular biology, the development of novel vaccines, approaches to bioreactor design and operation, monitoring techniques in process control and quality related topics. The work was carefully put together as one result of the Cell Culture Engineering IV Meeting held in San Diego, U.S.A. in 1994, however, the book may not be perceived as a proceedings volume - the criteria of the book series apply.
For cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment, as well as in (biotechnology or pharmaceutical) industry.

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Cell Culture Engineering IV, Improvements of Human Health covers the latest approaches to improving the cell host through improved understanding of the molecular biology, the development of novel vaccines, approaches to bioreactor design and operation, monitoring techniques in process control and quality related topics. The work was carefully put together as one result of the Cell Culture Engineering IV Meeting held in San Diego, U.S.A. in 1994, however, the book may not be perceived as a proceedings volume - the criteria of the book series apply.
For cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment, as well as in (biotechnology or pharmaceutical) industry.
Content:
Front Matter....Pages i-vii
Introduction....Pages 1-2
Cultural and physiological factors affecting expression of recombinant proteins....Pages 3-9
Selecting and designing cell lines for improved physiological characteristics....Pages 11-16
Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor....Pages 17-29
Diverse effects of essential (n-6 and n-3) fatty acids on cultured cells....Pages 31-50
Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production....Pages 51-56
Growth rate suppression of cultured mammalian cells enhances protein productivity....Pages 57-64
Growth and interferon-? production in batch culture of CHO cells....Pages 65-71
Metabolic and kinetic studies of hybridomas in exponentially fed-batch cultures using T-flasks....Pages 73-86
Adaptation of mammalian cells to non-ammoniagenic media....Pages 87-94
Effect of endogenous proteins on growth and antibody productivity in hybridoma batch cultures....Pages 95-102
Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process....Pages 103-109
Low temperature cultivation — A step towards process optimisation....Pages 111-116
Induction of apoptosis in oxygen-deprived cultures of hybridoma cells....Pages 117-128
Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures....Pages 129-138
Recombinant protein expression in a Drosophila cell line: comparison with the baculovirus system....Pages 139-144
Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells....Pages 145-155
Relationship between oxygen uptake rate and time of infection of Sf9 insect cells infected with a recombinant baculovirus....Pages 157-167
Optimization of vaccine production for animal health....Pages 169-176
Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected insect cells....Pages 177-186
The analysis of protein pharmaceuticals: Near future advances....Pages 187-194
The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin....Pages 195-208
Effect of lipid supplements on the production and glycosylation of recombinant interferon-? expressed in CHO cells....Pages 209-215
Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase for hFSH produced by recombinant CHO cells....Pages 217-221
Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines....Pages 223-228
Interaction of cell culture with downstream purification: a case study....Pages 229-242
Evaluation of membranes for use in on-line cell separation during mammalian cell perfusion processes....Pages 243-251
Vortex flow filtration of mammalian and insect cells....Pages 253-258
Automated imunoanalysis systems for monitoring mammalian cell cultivation processes....Pages 259-269
A direct computer control concept for mammalian cell fermentation processes....Pages 271-279
Automated monitoring of cell concentration and viability using an image analysis system....Pages 281-289
Software sensors for the monitoring of perfusion cultures: Evaluation of the hybridoma density and the medium composition from glucose concentration measurements....Pages 291-299
Design of a bubble-swarm bioreactor for animal cell culture....Pages 301-309
Cells and bubbles in sparged bioreactors....Pages 311-320
Quantitative investigations of cell-bubble interactions using a foam fractionation technique....Pages 321-328
Prediction of mechanical damage to animal cells in turbulence....Pages 329-335
Repeated-batch cultures of Baby Hamster Kidney cell aggregates in stirred vessels....Pages 337-349
Towards the development of a bioartificial pancreas: immunoisolation and NMR monitoring of mouse insulinomas....Pages 351-363
Overview of a quality assurance/quality control compliance program consistent with FDA regulations and policies for somatic cell and gene therapies: a four year experience....Pages 365-372
Back Matter....Pages 373-376

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Cell Culture Engineering IV, Improvements of Human Health covers the latest approaches to improving the cell host through improved understanding of the molecular biology, the development of novel vaccines, approaches to bioreactor design and operation, monitoring techniques in process control and quality related topics. The work was carefully put together as one result of the Cell Culture Engineering IV Meeting held in San Diego, U.S.A. in 1994, however, the book may not be perceived as a proceedings volume - the criteria of the book series apply.
For cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment, as well as in (biotechnology or pharmaceutical) industry.
Content:
Front Matter....Pages i-vii
Introduction....Pages 1-2
Cultural and physiological factors affecting expression of recombinant proteins....Pages 3-9
Selecting and designing cell lines for improved physiological characteristics....Pages 11-16
Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor....Pages 17-29
Diverse effects of essential (n-6 and n-3) fatty acids on cultured cells....Pages 31-50
Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production....Pages 51-56
Growth rate suppression of cultured mammalian cells enhances protein productivity....Pages 57-64
Growth and interferon-? production in batch culture of CHO cells....Pages 65-71
Metabolic and kinetic studies of hybridomas in exponentially fed-batch cultures using T-flasks....Pages 73-86
Adaptation of mammalian cells to non-ammoniagenic media....Pages 87-94
Effect of endogenous proteins on growth and antibody productivity in hybridoma batch cultures....Pages 95-102
Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process....Pages 103-109
Low temperature cultivation — A step towards process optimisation....Pages 111-116
Induction of apoptosis in oxygen-deprived cultures of hybridoma cells....Pages 117-128
Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures....Pages 129-138
Recombinant protein expression in a Drosophila cell line: comparison with the baculovirus system....Pages 139-144
Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells....Pages 145-155
Relationship between oxygen uptake rate and time of infection of Sf9 insect cells infected with a recombinant baculovirus....Pages 157-167
Optimization of vaccine production for animal health....Pages 169-176
Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected insect cells....Pages 177-186
The analysis of protein pharmaceuticals: Near future advances....Pages 187-194
The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin....Pages 195-208
Effect of lipid supplements on the production and glycosylation of recombinant interferon-? expressed in CHO cells....Pages 209-215
Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase for hFSH produced by recombinant CHO cells....Pages 217-221
Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines....Pages 223-228
Interaction of cell culture with downstream purification: a case study....Pages 229-242
Evaluation of membranes for use in on-line cell separation during mammalian cell perfusion processes....Pages 243-251
Vortex flow filtration of mammalian and insect cells....Pages 253-258
Automated imunoanalysis systems for monitoring mammalian cell cultivation processes....Pages 259-269
A direct computer control concept for mammalian cell fermentation processes....Pages 271-279
Automated monitoring of cell concentration and viability using an image analysis system....Pages 281-289
Software sensors for the monitoring of perfusion cultures: Evaluation of the hybridoma density and the medium composition from glucose concentration measurements....Pages 291-299
Design of a bubble-swarm bioreactor for animal cell culture....Pages 301-309
Cells and bubbles in sparged bioreactors....Pages 311-320
Quantitative investigations of cell-bubble interactions using a foam fractionation technique....Pages 321-328
Prediction of mechanical damage to animal cells in turbulence....Pages 329-335
Repeated-batch cultures of Baby Hamster Kidney cell aggregates in stirred vessels....Pages 337-349
Towards the development of a bioartificial pancreas: immunoisolation and NMR monitoring of mouse insulinomas....Pages 351-363
Overview of a quality assurance/quality control compliance program consistent with FDA regulations and policies for somatic cell and gene therapies: a four year experience....Pages 365-372
Back Matter....Pages 373-376
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