Ebook: A Laboratory Guide to Glycoconjugate Analysis
- Tags: Life Sciences general, Biomedicine general
- Series: BioMethods
- Year: 1995
- Publisher: Birkhäuser Basel
- Edition: 1
- Language: English
- pdf
18. 2 Principle of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. 3 Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. 4 Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 Measurement of Binding Constant for the Interaction Between Protein and Native Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ The Application of Capillary Affinity Electrophoresis to the Analysis _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 Principle of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. 3 Determination of Association Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. 4 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 General considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. 5 Limitations of the Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 370 19. 6 Application of CAE to the Analysis of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. 3 Sample Detection and Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Sample detection by blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. 4 Analysis of Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring sample mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical analysis of data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 Interpreting ACE patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 Reverse ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. 5 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399 XII List of Contributors Nebojsa Avdalovic John T. Gallagher Dionex Corporation Cancer Research Campaign Department of Medical Oncology 445 Lakeside Drive University of Manchester Sunnyvale, CA 94086 Christie CRC Research Centre Klaus Biemann Wilmslow Road Department of Chemistry Manchester M20 4BX Massachusetts Institute of Technology UK Cambridge, MA 02139-4307 USA Geoffrey R.
Content:
Front Matter....Pages I-XXII
High-Performance Liquid Chromatography of Derivatized and Non-Derivatized Oligosaccharides: A Review....Pages 1-22
Detection of the Presence of Carbohydrates in Glycoproteins....Pages 23-39
HPAE-PAD Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride Membranes....Pages 41-59
Determination of the Disaccharide Composition of Glycosaminoglycans: Comparison of Chemical and Enzymatic Scission....Pages 61-76
Mass Spectrometric Analysis of Highly Acidic Polysaccharides....Pages 77-89
Analysis of N-Glycans by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry....Pages 91-112
Polyacrylamide Gel Electrophoresis of Fluorophore-Labelled Reducing Saccharides: A Review....Pages 113-139
Carbohydrate Analysis with Capillary Electrophoresis....Pages 141-160
Oligosaccharide Profiling of Keratan Sulphate....Pages 161-181
Analysis of the Structure of Heparin and Heparan Sulfate by High-Resolution Separation of Oligosaccharides....Pages 183-197
HPLC Strategies for Profiling and Sequencing Oligosaccharides....Pages 199-234
Enzymatic Sequence Analysis of Glycoprotein Glycans....Pages 235-260
Exosequencing of Heparan Sulphate/Heparin Saccharides Using Lysosomal Enzymes....Pages 261-277
The Application of Two-Dimensional Capillary Electrophoresis to the Identification of Glycan Structures....Pages 279-293
The Application of Three-Dimensional HPLC to the Identification of N-Linked Oligosaccharide Structures....Pages 295-306
Simultaneous Fluorescent Labelling and Biotinylation of Oligosaccharides: A Versatile Approach to the Analysis of Oligosaccharide Structure and Function....Pages 307-328
Preparation of Neoglycolipids for Structure and Function Assignments of Oligosaccharides....Pages 329-348
Analysis of Protein — Carbohydrate Interactions by FACE/Gel Retardation Assay....Pages 349-360
The Application of Capillary Affinity Electrophoresis to the Analysis of Carbohydrate-Protein Interactions....Pages 361-377
Analysis of Protein-Glycosaminoglycan Interactions by Affinity Co-Electrophoresis....Pages 379-398
Back Matter....Pages 399-410
Content:
Front Matter....Pages I-XXII
High-Performance Liquid Chromatography of Derivatized and Non-Derivatized Oligosaccharides: A Review....Pages 1-22
Detection of the Presence of Carbohydrates in Glycoproteins....Pages 23-39
HPAE-PAD Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride Membranes....Pages 41-59
Determination of the Disaccharide Composition of Glycosaminoglycans: Comparison of Chemical and Enzymatic Scission....Pages 61-76
Mass Spectrometric Analysis of Highly Acidic Polysaccharides....Pages 77-89
Analysis of N-Glycans by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry....Pages 91-112
Polyacrylamide Gel Electrophoresis of Fluorophore-Labelled Reducing Saccharides: A Review....Pages 113-139
Carbohydrate Analysis with Capillary Electrophoresis....Pages 141-160
Oligosaccharide Profiling of Keratan Sulphate....Pages 161-181
Analysis of the Structure of Heparin and Heparan Sulfate by High-Resolution Separation of Oligosaccharides....Pages 183-197
HPLC Strategies for Profiling and Sequencing Oligosaccharides....Pages 199-234
Enzymatic Sequence Analysis of Glycoprotein Glycans....Pages 235-260
Exosequencing of Heparan Sulphate/Heparin Saccharides Using Lysosomal Enzymes....Pages 261-277
The Application of Two-Dimensional Capillary Electrophoresis to the Identification of Glycan Structures....Pages 279-293
The Application of Three-Dimensional HPLC to the Identification of N-Linked Oligosaccharide Structures....Pages 295-306
Simultaneous Fluorescent Labelling and Biotinylation of Oligosaccharides: A Versatile Approach to the Analysis of Oligosaccharide Structure and Function....Pages 307-328
Preparation of Neoglycolipids for Structure and Function Assignments of Oligosaccharides....Pages 329-348
Analysis of Protein — Carbohydrate Interactions by FACE/Gel Retardation Assay....Pages 349-360
The Application of Capillary Affinity Electrophoresis to the Analysis of Carbohydrate-Protein Interactions....Pages 361-377
Analysis of Protein-Glycosaminoglycan Interactions by Affinity Co-Electrophoresis....Pages 379-398
Back Matter....Pages 399-410
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