Ebook: Liver Carcinogenesis: The Molecular Pathways

During the last years much attention has been given to the investigation of the roles of specific growth modulators acting on liver cell populations. HGF functions as a pleotropic factor in the liver as well as in other extrahepatic tissues. The hepatic stem cell compartment is thought to play a major role in liver tissue restoration. Mechanisms of cell growth and cell death of the liver are described in detail, with particular emphasis on apoptosis. The functions of HBV-pX protein and the roles of hepatitis B virus gene expression relation to hepatocellular carcinoma are discussed. Techniques for obtaining human and nonhuman hepatocytes and other liver cell types and for efficient transfer and expression of genes in hepatocytes and liver-derived cell lines are presented.

---

During the last years much attention has been given to the investigation of the roles of specific growth modulators acting on liver cell populations. HGF functions as a pleotropic factor in the liver as well as in other extrahepatic tissues. The hepatic stem cell compartment is thought to play a major role in liver tissue restoration. Mechanisms of cell growth and cell death of the liver are described in detail, with particular emphasis on apoptosis. The functions of HBV-pX protein and the roles of hepatitis B virus gene expression relation to hepatocellular carcinoma are discussed. Techniques for obtaining human and nonhuman hepatocytes and other liver cell types and for efficient transfer and expression of genes in hepatocytes and liver-derived cell lines are presented.

---

During the last years much attention has been given to the investigation of the roles of specific growth modulators acting on liver cell populations. HGF functions as a pleotropic factor in the liver as well as in other extrahepatic tissues. The hepatic stem cell compartment is thought to play a major role in liver tissue restoration. Mechanisms of cell growth and cell death of the liver are described in detail, with particular emphasis on apoptosis. The functions of HBV-pX protein and the roles of hepatitis B virus gene expression relation to hepatocellular carcinoma are discussed. Techniques for obtaining human and nonhuman hepatocytes and other liver cell types and for efficient transfer and expression of genes in hepatocytes and liver-derived cell lines are presented.
Content:
Front Matter....Pages I-XIX
Molecular Pathways in Hepatocyte Proliferation....Pages 1-11
HGF and Liver Growth Regulation....Pages 13-31
Structure, Pleiotropic Actions, and Organotrophic Roles of Hepatocyte Growth Factor....Pages 33-53
Hepatocyte Growth Factor and Its Variant with a Deletion of Five Amino Acids are Distinguishable in Biological, Physicochemical, and Immunochemical Properties....Pages 55-70
Growth Stimulation Mediated by G Protein Coupled Receptors in Hepatocytes: Synergism with Epidermal Growth Factor and Mechanisms of Signal Transduction....Pages 71-91
Protein Tyrosine Phosphatases in Signal Transduction....Pages 93-108
C-Myc is Essential for Initiation of Dna Synthesis in EGF-Stimulated Hepatocyte Cultures....Pages 109-127
Cellular Biology of the Rat Hepatic Stem Cell Compartment....Pages 129-145
Changes in Protein Expression During Oval Cell Proliferation in the Liver....Pages 147-162
Stem Cell Activation in the Acetylaminofluorene-Treated Regenerating Rat Liver: A Bile Ductular Reaction?....Pages 163-180
Apoptosis and Its Role in Hepatic Carcinogenesis by Non-Genotoxic Agents....Pages 181-195
Cell Proliferation and Cell Death in the Liver: Patterns, Mechanisms and Measurements....Pages 197-214
Preneoplastic Changes During Non-Genotoxic Hepatocarcinogenesis....Pages 215-229
mRNA Composition of Rat Liver Tumors Initiated by Aromatic Amines....Pages 231-247
Liver ?-Glutamyl Transpeptidase Activity after Cyclosporine A and Amlodipine Treatment....Pages 249-260
Enhanced Expression of the 27 Da Heat-Shock Protein During Dena-Induced Hepatocarcinogenesis in Rat and in Human Neoplastic and Nonneoplastic Liver Tissues....Pages 261-273
Hepatic Recovery of Dimethylnitrosamine-Cirrhotic Rats after Injection of the Liver Growth Factor....Pages 275-285
Cell Contact-Mediated Regulation of Hepatocyte Differentiation/Proliferation: Role of LRP(s)....Pages 287-299
Interactions of Human Hepatocytes with Hepatitis B Virus....Pages 301-309
Liver-Specific Aspects of Hepatitis B Virus Gene Expression....Pages 311-339
Transcriptional involvement of the Hepatitis B Virus Protein in Cellular Transduction Systems. Protein-Protein Interactions with Bzip transactivators....Pages 341-358
Role of Duck Hepatitis B Virus Infection, Aflatoxin B1 and p53 Mutation in Hepatocellular Carcinomas of Ducks....Pages 359-371
The Preparation and Culturing of Rat Hepatocytes....Pages 373-387
Isolation and Growth of Hepatocytes and Biliary Epithelial Cells from Normal and Diseased Human Livers....Pages 389-409
A Novel Strategy for Isolating Hepr, a Human Small Intestinal Cytidine Deaminase....Pages 411-419
Regenerating Liver: Isolation of Up- and Down-Regulated Gene Products....Pages 421-434
Hepatotrophic and Renotrophic Activities of HGF In Vivo: Possible Application of HGF for Hepatic and Renal Diseases....Pages 435-448
Targeted Gene Delivery And Expression....Pages 449-456
The Generation of Tumor Vaccines by Adenovirus-Enhanced Transferrinfection of Cytokine Genes into Tumor Cells....Pages 457-466
Studies on Persistence and Enhancement of Targeted Gene Expression....Pages 467-479
Back Matter....Pages 481-491

---

During the last years much attention has been given to the investigation of the roles of specific growth modulators acting on liver cell populations. HGF functions as a pleotropic factor in the liver as well as in other extrahepatic tissues. The hepatic stem cell compartment is thought to play a major role in liver tissue restoration. Mechanisms of cell growth and cell death of the liver are described in detail, with particular emphasis on apoptosis. The functions of HBV-pX protein and the roles of hepatitis B virus gene expression relation to hepatocellular carcinoma are discussed. Techniques for obtaining human and nonhuman hepatocytes and other liver cell types and for efficient transfer and expression of genes in hepatocytes and liver-derived cell lines are presented.
Content:
Front Matter....Pages I-XIX
Molecular Pathways in Hepatocyte Proliferation....Pages 1-11
HGF and Liver Growth Regulation....Pages 13-31
Structure, Pleiotropic Actions, and Organotrophic Roles of Hepatocyte Growth Factor....Pages 33-53
Hepatocyte Growth Factor and Its Variant with a Deletion of Five Amino Acids are Distinguishable in Biological, Physicochemical, and Immunochemical Properties....Pages 55-70
Growth Stimulation Mediated by G Protein Coupled Receptors in Hepatocytes: Synergism with Epidermal Growth Factor and Mechanisms of Signal Transduction....Pages 71-91
Protein Tyrosine Phosphatases in Signal Transduction....Pages 93-108
C-Myc is Essential for Initiation of Dna Synthesis in EGF-Stimulated Hepatocyte Cultures....Pages 109-127
Cellular Biology of the Rat Hepatic Stem Cell Compartment....Pages 129-145
Changes in Protein Expression During Oval Cell Proliferation in the Liver....Pages 147-162
Stem Cell Activation in the Acetylaminofluorene-Treated Regenerating Rat Liver: A Bile Ductular Reaction?....Pages 163-180
Apoptosis and Its Role in Hepatic Carcinogenesis by Non-Genotoxic Agents....Pages 181-195
Cell Proliferation and Cell Death in the Liver: Patterns, Mechanisms and Measurements....Pages 197-214
Preneoplastic Changes During Non-Genotoxic Hepatocarcinogenesis....Pages 215-229
mRNA Composition of Rat Liver Tumors Initiated by Aromatic Amines....Pages 231-247
Liver ?-Glutamyl Transpeptidase Activity after Cyclosporine A and Amlodipine Treatment....Pages 249-260
Enhanced Expression of the 27 Da Heat-Shock Protein During Dena-Induced Hepatocarcinogenesis in Rat and in Human Neoplastic and Nonneoplastic Liver Tissues....Pages 261-273
Hepatic Recovery of Dimethylnitrosamine-Cirrhotic Rats after Injection of the Liver Growth Factor....Pages 275-285
Cell Contact-Mediated Regulation of Hepatocyte Differentiation/Proliferation: Role of LRP(s)....Pages 287-299
Interactions of Human Hepatocytes with Hepatitis B Virus....Pages 301-309
Liver-Specific Aspects of Hepatitis B Virus Gene Expression....Pages 311-339
Transcriptional involvement of the Hepatitis B Virus Protein in Cellular Transduction Systems. Protein-Protein Interactions with Bzip transactivators....Pages 341-358
Role of Duck Hepatitis B Virus Infection, Aflatoxin B1 and p53 Mutation in Hepatocellular Carcinomas of Ducks....Pages 359-371
The Preparation and Culturing of Rat Hepatocytes....Pages 373-387
Isolation and Growth of Hepatocytes and Biliary Epithelial Cells from Normal and Diseased Human Livers....Pages 389-409
A Novel Strategy for Isolating Hepr, a Human Small Intestinal Cytidine Deaminase....Pages 411-419
Regenerating Liver: Isolation of Up- and Down-Regulated Gene Products....Pages 421-434
Hepatotrophic and Renotrophic Activities of HGF In Vivo: Possible Application of HGF for Hepatic and Renal Diseases....Pages 435-448
Targeted Gene Delivery And Expression....Pages 449-456
The Generation of Tumor Vaccines by Adenovirus-Enhanced Transferrinfection of Cytokine Genes into Tumor Cells....Pages 457-466
Studies on Persistence and Enhancement of Targeted Gene Expression....Pages 467-479
Back Matter....Pages 481-491
....