Ebook: Chromosomal Alterations: Origin and Significance

Due to sensitive molecular biological techniques, our understanding of chromosomal aberrations is steadily increasing. Provided here is a review of basic and applied aspects of the field. Chromosome structure, induction of DNA lesions by different clastogenic agents and their repair, induction of aberrations by agents which affect specific sequences in the DNA, and factors affecting induction and yield of chromosomal aberrations are covered.
Further, topics such as automation of aberration scoring, problems associated with using chromosomal aberrations and micronuclei in population monitoring and the importance of chromosomal aberration assays in mutagenicity testing of chemicals are included.

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Due to sensitive molecular biological techniques, our understanding of chromosomal aberrations is steadily increasing. Provided here is a review of basic and applied aspects of the field. Chromosome structure, induction of DNA lesions by different clastogenic agents and their repair, induction of aberrations by agents which affect specific sequences in the DNA, and factors affecting induction and yield of chromosomal aberrations are covered.
Further, topics such as automation of aberration scoring, problems associated with using chromosomal aberrations and micronuclei in population monitoring and the importance of chromosomal aberration assays in mutagenicity testing of chemicals are included.

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Due to sensitive molecular biological techniques, our understanding of chromosomal aberrations is steadily increasing. Provided here is a review of basic and applied aspects of the field. Chromosome structure, induction of DNA lesions by different clastogenic agents and their repair, induction of aberrations by agents which affect specific sequences in the DNA, and factors affecting induction and yield of chromosomal aberrations are covered.
Further, topics such as automation of aberration scoring, problems associated with using chromosomal aberrations and micronuclei in population monitoring and the importance of chromosomal aberration assays in mutagenicity testing of chemicals are included.
Content:
Front Matter....Pages I-XI
UV-Induced Pyrimidine Monoadducts and their in vivo Photofootprints....Pages 1-9
Chromatin Structure, Hyperthermia and Repair of UV-Induced DNA Photolesions in Mammalian Cells....Pages 10-20
An Inherited Homogeneously Staining Region Derived from a Long-Range Repeat Family in the House Mouse....Pages 21-30
Detection of Genetic Imbalances in Tumor Genomes by Fluorescence in situ Hybridization with Tumor Genomic DNA and Subregional DNA Probes....Pages 31-41
Fluorescent in situ Hybridization (FISH) in Cytogenetical Studies....Pages 42-49
The Use of Premature Chromosome Condensation and Chromosome Painting to Understand Chromosome Exchange Formation....Pages 50-56
The Role of DNA Double-Strand-Break Rejoining in Chromosome Damage and Repair....Pages 57-63
Investigations of Aberration Origins Using BrdUrd....Pages 64-75
Elucidation of Some Factors Involved in the Formation of Chromosomal Aberrations by Inhibiting the Repair Polymerase....Pages 76-91
Use of Antitopoisomerase Drugs to Study the Mechanisms of Induction of Chromosomal Damage....Pages 92-102
Search for Perturbations induced by X-irradiation in the G2 Phase of Human Lymphocytes....Pages 103-115
A Comparison of Radiation-Induced Aberrations in Human Cells Involving Early and Late Replicating X-Chromosomes....Pages 116-124
Radioprotective Chemicals as Tools for Studying Mechanisms of Radiation-Induced Chromosome Damage in Human Lymphocytes....Pages 125-131
Factors Determining the Yields of Radiation-Induced Chromosomal Aberrations as Visualized by Means of Premature Chromosome Condensation in Interphase Cells....Pages 132-139
Synergism and Adaptive Response in the Interaction of Low Dose Irradiation with Subsequent Mutagenic Treatment in G2 Phase Human Lymphocytes....Pages 140-149
Responses of Radiosensitive Mutant Mammalian Cell Lines to Restriction Endonuclease induced DNA Double-Strand Breaks....Pages 150-159
Quantitative Localization of Chromatid Breaks Induced by Alu I in the Long Arms of Chromosomes Number 1 of Chinese Hamster Ovary (CHO) Cells by Microphotometric Scanning....Pages 160-168
“Life time” of AluI inside Glycerol-Induced Vesicles in CHO Cells....Pages 169-183
Progress in Automatic Dicentric Hunting....Pages 184-191
The Micronucleus Assay with Rodent Peripheral Blood and Acridine Orange Supravital Staining....Pages 192-202
The Formation of Micronuclei after Exposure to Ionizing Radiation....Pages 203-213
Excision Repaired Sites, Chromosome Breaks and Chromosome Loss Measured Simultaneously in Human Lymphocytes using the Cytokinesis Block Micronucleus Assay and Cytosine Arabinoside....Pages 214-222
The Potential of FISH for Meiotic Segregation Analysis....Pages 223-234
Reliable Chromosome Studies of Human Oocytes and Spermatozoa using the Gradual Fixation-Air Drying (GF-AD) Method....Pages 235-251
Nondisjunction by Failures in the Molecular Control of Oocyte Maturation....Pages 252-261
Cytogenetic and Molecular Investigations in Chromosomal Instability Syndromes....Pages 262-268
Distributions of Spontaneous Chromosomal Aberrations and of Spontaneous and Induced SCE and Micronuclei in Peripheral Lymphocytes from a Human Population....Pages 269-281
Does the Genetic Deficiency in ALDH2 Determine the Alcohol-Drinking Behavior and the Induction of Chromosome Alterations in Peripheral Lymphocytes by Alcohol?....Pages 282-292
Variability of Chromosomal Alterations in Human Peripheral Lymphocytes of Smokers and Nonsmokers....Pages 293-306
Chromosome Analysis in Accidental, Occupational and Environmental Radiation Exposure....Pages 307-318
New Approaches to Design and Interpretation of in vitro Chromosomal Aberration Tests....Pages 319-332
Comments on Short-Term Cytogenetic Assays for Screening of Environmental Chemical Carcinogens....Pages 333-342
Localized Chromosomal Aberrations in the Heterochromatic q Arm of the X Chromosome in V79 Chinese Hamster Cells and the Implications for Industrial in vitro Cytogenetic Screening....Pages 343-347
Cytogenetic Studies in Laboratory Animals Exposed by Inhalation to Mainstream Smoke or Environmental Tobacco Smoke....Pages 348-361
Evolutionary Aspects of Structural Chromosome Aberrations....Pages 362-379
Back Matter....Pages 380-394
....Pages 395-402