Ebook: Identification of Transcribed Sequences

The Human Genome Project, an endeavor to map and sequence the entire human genome, has been in existence for almost seven years. One result of this effort has been the development of increasingly detailed genetic and physical maps spanning large regions of virtually every chromosome. Paralleling this has been the increasingly high resolution mapping of many &wnetic diseases. Together, these developments have facilitated the isolation of specific disease genes and are now motivating the construction of comprehensive transcriptional maps. This latter endeavor represents a new facet of the genome project, and as such requires the recognition and solution of a new set of problems, with the attendant development and application of a new set of techniques. The First International Workshop on the Identification of Transcribed Sequences in the Human Genome was held in 1991 and was attended by 23 investigators. Discussions at this meeting were largely devoted to defining the magnitude of the problem and describing the available techniques. A small number of laboratories reported the development of new techniques (at that time, for example, exon trapping, cDNA hybrid selection, direct cDNA screening, use of splice junction conserved sequences,etc.), but data were too limited to permit comparisons of their relative efficiencies.

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From Genomic DNA to Transcribed Sequences: Classical Approaches: Use of cDNA Selection and Evolutionarily Conserved Sequences to Isolate Transcribed Sequences from Region Xp11.21; E.N. Burright, et al. Identification of cDNAs by Direct Hybridization Using Cosmid Probes; G.G. Lennon, K. Lieuallen.Hybridization Based Approaches:Hybridization Selection: Towards a Transcriptional Map of Human Chromosome 21; K. Gardiner, et al. Variations on Hybridization Selection. Direct cDNA Screening. Exon Trapping: An Exon Trapping System Providing Size Selection of Spliced Clones and Facilitating Direct Cloning; N.A. Datson, et al. Computer Based Approaches: Shallow Shotgun Sequencing as a Strategy for Finding Coding Exons; J.M. Claverie.From Transcribed Sequences To GenomicLocalization:Generating and Sequencing cDNAs: Requirements in Screening cDNA Libraries for New Genes and Solutions Offered by SBH Technology; R. Drmanac, et al.Mapping cDNAs: Mapping cDNAs by Hybridization to Gridded Arrays of DNA from YAC Clones; D.R. Moir, et al. 19 additional articles. Index.

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From Genomic DNA to Transcribed Sequences: Classical Approaches: Use of cDNA Selection and Evolutionarily Conserved Sequences to Isolate Transcribed Sequences from Region Xp11.21; E.N. Burright, et al. Identification of cDNAs by Direct Hybridization Using Cosmid Probes; G.G. Lennon, K. Lieuallen.Hybridization Based Approaches:Hybridization Selection: Towards a Transcriptional Map of Human Chromosome 21; K. Gardiner, et al. Variations on Hybridization Selection. Direct cDNA Screening. Exon Trapping: An Exon Trapping System Providing Size Selection of Spliced Clones and Facilitating Direct Cloning; N.A. Datson, et al. Computer Based Approaches: Shallow Shotgun Sequencing as a Strategy for Finding Coding Exons; J.M. Claverie.From Transcribed Sequences To GenomicLocalization:Generating and Sequencing cDNAs: Requirements in Screening cDNA Libraries for New Genes and Solutions Offered by SBH Technology; R. Drmanac, et al.Mapping cDNAs: Mapping cDNAs by Hybridization to Gridded Arrays of DNA from YAC Clones; D.R. Moir, et al. 19 additional articles. Index.
Content:
Requirements in Screening cDNA Libraries for New Genes and Solutions Offered by SBH Technology....Pages 239-251
Establishing Catalogues of Expressed Sequences by Oligonucleotide Fingerprinting of cDNA Libraries....Pages 253-260
PCR-Based Technologies to Study Differential Gene Expression in Rat Brain....Pages 261-271
259 Human Brain Expressed Sequence Tags (ESTs): Chromosome Localization, Subregional Assignment, and Sequence Analysis....Pages 273-288
Mapping cDNAs by Hybridization to Gridded Arrays of DNA from YAC Clones....Pages 289-297
Discussion....Pages 299-302
Back Matter....Pages 303-304

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From Genomic DNA to Transcribed Sequences: Classical Approaches: Use of cDNA Selection and Evolutionarily Conserved Sequences to Isolate Transcribed Sequences from Region Xp11.21; E.N. Burright, et al. Identification of cDNAs by Direct Hybridization Using Cosmid Probes; G.G. Lennon, K. Lieuallen.Hybridization Based Approaches:Hybridization Selection: Towards a Transcriptional Map of Human Chromosome 21; K. Gardiner, et al. Variations on Hybridization Selection. Direct cDNA Screening. Exon Trapping: An Exon Trapping System Providing Size Selection of Spliced Clones and Facilitating Direct Cloning; N.A. Datson, et al. Computer Based Approaches: Shallow Shotgun Sequencing as a Strategy for Finding Coding Exons; J.M. Claverie.From Transcribed Sequences To GenomicLocalization:Generating and Sequencing cDNAs: Requirements in Screening cDNA Libraries for New Genes and Solutions Offered by SBH Technology; R. Drmanac, et al.Mapping cDNAs: Mapping cDNAs by Hybridization to Gridded Arrays of DNA from YAC Clones; D.R. Moir, et al. 19 additional articles. Index.
Content:
Requirements in Screening cDNA Libraries for New Genes and Solutions Offered by SBH Technology....Pages 239-251
Establishing Catalogues of Expressed Sequences by Oligonucleotide Fingerprinting of cDNA Libraries....Pages 253-260
PCR-Based Technologies to Study Differential Gene Expression in Rat Brain....Pages 261-271
259 Human Brain Expressed Sequence Tags (ESTs): Chromosome Localization, Subregional Assignment, and Sequence Analysis....Pages 273-288
Mapping cDNAs by Hybridization to Gridded Arrays of DNA from YAC Clones....Pages 289-297
Discussion....Pages 299-302
Back Matter....Pages 303-304
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