Ebook: Molecular Biology of Mitochondrial Transport Systems

Mitochondrial transport systems are essential to mitochondrial function and therefore to energy homeostasis within the cell.
The book contains studies utilizing the techniques of biochemistry, physiology, molecular biology and genetics to reveal the structure and function of mitochondrial transport systems.
It is divided into the following six sections:
- Proton Translocation: The Uncoupling Protein and the ATPase; - Carriers and Transporters; - Mitochondrial Ion Channels; Structure of the Outer Mitochondrial Membrane Channel, VDAC; - VDAC, Peripheral Kinases and Energy Utilization; - Mitochondrial Channels in Humans and Relationship to Disease.

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Mitochondrial transport systems are essential to mitochondrial function and therefore to energy homeostasis within the cell.
The book contains studies utilizing the techniques of biochemistry, physiology, molecular biology and genetics to reveal the structure and function of mitochondrial transport systems.
It is divided into the following six sections:
- Proton Translocation: The Uncoupling Protein and the ATPase; - Carriers and Transporters; - Mitochondrial Ion Channels; Structure of the Outer Mitochondrial Membrane Channel, VDAC; - VDAC, Peripheral Kinases and Energy Utilization; - Mitochondrial Channels in Humans and Relationship to Disease.

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Mitochondrial transport systems are essential to mitochondrial function and therefore to energy homeostasis within the cell.
The book contains studies utilizing the techniques of biochemistry, physiology, molecular biology and genetics to reveal the structure and function of mitochondrial transport systems.
It is divided into the following six sections:
- Proton Translocation: The Uncoupling Protein and the ATPase; - Carriers and Transporters; - Mitochondrial Ion Channels; Structure of the Outer Mitochondrial Membrane Channel, VDAC; - VDAC, Peripheral Kinases and Energy Utilization; - Mitochondrial Channels in Humans and Relationship to Disease.
Content:
Front Matter....Pages I-XII
Molecular Studies of the Mitochondrial Uncoupling Protein....Pages 1-9
Structure-Function Relationship in the Uncoupling Protein of Brown Adipose Tissue....Pages 11-18
The Gating and Coupling Function of Mitochondrial, H+ ATP Synthase. Role of Fo and F1 Subunits....Pages 19-39
Extension of the Mitochondrial Transporter Family....Pages 41-53
The Tricarboxylate Carrier....Pages 55-65
The Mitochondrial Pyruvate Carrier: The Mechanism of Substrate Binding....Pages 67-79
Membrane Topology of the ADP/ATP Carrier from Mitochondria....Pages 81-97
The Use of Ligand Binding Techniques to Identify Transport Proteins: The Characterization of the [125I]-Iodophenylarsine Oxide Receptor in Rat Mitochondrial Membranes....Pages 99-116
Channel Properties of Mitochondrial Carriers....Pages 117-129
A Minimal Kinetic Model of the Activity of the 107 pS Channel of the Inner Membrane of Mitochondria....Pages 131-136
A Channel Model to Explain Regulation of the Mitochondrial Inner Membrane Anion Channel (IMAC)....Pages 137-151
The Mitochondrial Permeabilization Pore....Pages 153-168
Mitochondrial Channels: An Integrated View....Pages 169-198
Purification and Patch Clamp Analysis of Two Mitochondrial Channels....Pages 199-208
The Mitochondrial Outer Membrane Contains at Least Two Distinct Channels....Pages 209-219
ATP-Sensitive K+ Channel in Rat Liver Mitochondria: Functional Characteristics....Pages 221-228
Probing for the Voltage Sensor in a Mitochondrial Channel, VDAC, Using Site-Directed Mutagenesis....Pages 229-248
Insights Into the Structure of the Mitochondrial Channel, VDAC, Provided by Electron Microscopy....Pages 249-263
Experimental Supports to a Sixteen-Strands Model of Transmembrane Arrangement of Mitochondrial Porin and Preliminary Results Concerning a Multigene Family in Drosophila melanogaster Related to Human Mitochondrial Porin....Pages 265-280
Structure and Function of the VDAC Ion Channel....Pages 281-296
The Function of the Mitochondrial Outer Membrane Pore in the Regulation of Peripheral Kinases and Energy Metabolism....Pages 297-311
Coordinated Regulation of Cerebral Glycolytic and Oxidative Metabolism Mediated by Mitochondrially Bound Hexokinase....Pages 313-325
Influence of Macromolecules on the Permeability of Porin Pores and Dynamic Compartmentation of Adenine Nucleotides in the Mitochondrial Intermembrane Space....Pages 327-340
Restrictions of Metabolite Permeation Through the Outer Mitochondrial Membrane of Porin-Deficient Yeast Mutant....Pages 341-356
Role of Porin-Kinase Interactions in Disease....Pages 357-377
Genes Encoding Human VDAC Proteins: Identification of Multiple Isoforms, Expression in Yeast and Chromosomal Localization....Pages 379-388
“Porin 31HL” in the Plasmalemma of Human Cells: A VDAC Discussed as Part of a Chloride Channel Complex in Normal and Cystic Fibrosis B-Lymphocyte Cell Lines....Pages 389-405
Back Matter....Pages 406-412

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Mitochondrial transport systems are essential to mitochondrial function and therefore to energy homeostasis within the cell.
The book contains studies utilizing the techniques of biochemistry, physiology, molecular biology and genetics to reveal the structure and function of mitochondrial transport systems.
It is divided into the following six sections:
- Proton Translocation: The Uncoupling Protein and the ATPase; - Carriers and Transporters; - Mitochondrial Ion Channels; Structure of the Outer Mitochondrial Membrane Channel, VDAC; - VDAC, Peripheral Kinases and Energy Utilization; - Mitochondrial Channels in Humans and Relationship to Disease.
Content:
Front Matter....Pages I-XII
Molecular Studies of the Mitochondrial Uncoupling Protein....Pages 1-9
Structure-Function Relationship in the Uncoupling Protein of Brown Adipose Tissue....Pages 11-18
The Gating and Coupling Function of Mitochondrial, H+ ATP Synthase. Role of Fo and F1 Subunits....Pages 19-39
Extension of the Mitochondrial Transporter Family....Pages 41-53
The Tricarboxylate Carrier....Pages 55-65
The Mitochondrial Pyruvate Carrier: The Mechanism of Substrate Binding....Pages 67-79
Membrane Topology of the ADP/ATP Carrier from Mitochondria....Pages 81-97
The Use of Ligand Binding Techniques to Identify Transport Proteins: The Characterization of the [125I]-Iodophenylarsine Oxide Receptor in Rat Mitochondrial Membranes....Pages 99-116
Channel Properties of Mitochondrial Carriers....Pages 117-129
A Minimal Kinetic Model of the Activity of the 107 pS Channel of the Inner Membrane of Mitochondria....Pages 131-136
A Channel Model to Explain Regulation of the Mitochondrial Inner Membrane Anion Channel (IMAC)....Pages 137-151
The Mitochondrial Permeabilization Pore....Pages 153-168
Mitochondrial Channels: An Integrated View....Pages 169-198
Purification and Patch Clamp Analysis of Two Mitochondrial Channels....Pages 199-208
The Mitochondrial Outer Membrane Contains at Least Two Distinct Channels....Pages 209-219
ATP-Sensitive K+ Channel in Rat Liver Mitochondria: Functional Characteristics....Pages 221-228
Probing for the Voltage Sensor in a Mitochondrial Channel, VDAC, Using Site-Directed Mutagenesis....Pages 229-248
Insights Into the Structure of the Mitochondrial Channel, VDAC, Provided by Electron Microscopy....Pages 249-263
Experimental Supports to a Sixteen-Strands Model of Transmembrane Arrangement of Mitochondrial Porin and Preliminary Results Concerning a Multigene Family in Drosophila melanogaster Related to Human Mitochondrial Porin....Pages 265-280
Structure and Function of the VDAC Ion Channel....Pages 281-296
The Function of the Mitochondrial Outer Membrane Pore in the Regulation of Peripheral Kinases and Energy Metabolism....Pages 297-311
Coordinated Regulation of Cerebral Glycolytic and Oxidative Metabolism Mediated by Mitochondrially Bound Hexokinase....Pages 313-325
Influence of Macromolecules on the Permeability of Porin Pores and Dynamic Compartmentation of Adenine Nucleotides in the Mitochondrial Intermembrane Space....Pages 327-340
Restrictions of Metabolite Permeation Through the Outer Mitochondrial Membrane of Porin-Deficient Yeast Mutant....Pages 341-356
Role of Porin-Kinase Interactions in Disease....Pages 357-377
Genes Encoding Human VDAC Proteins: Identification of Multiple Isoforms, Expression in Yeast and Chromosomal Localization....Pages 379-388
“Porin 31HL” in the Plasmalemma of Human Cells: A VDAC Discussed as Part of a Chloride Channel Complex in Normal and Cystic Fibrosis B-Lymphocyte Cell Lines....Pages 389-405
Back Matter....Pages 406-412
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