Ebook: Molecular Diagnostics of Cancer

Present and potential future applications of new diagnostic strategies basedon the direct or indirect detection of cancer genes are delineated in this volume. Among the methodological aspects covered are enzymatic target amplification by the polymerase chain reaction and related techniques, DNA fingerprinting, transfer of putative cancer genes in appropriate receipient cells, and recent developments inthe application of monoclonal antibodies in immunohistochemistry and immunoscintigraphy. The diagnostic and functional implications of mutations in cancer genes such as ras and p53 are described. The characterization of cancer genes and their products is correlated with growth control anddissemination of tumour cells by in vitro or clinical evidence. The contributions in the present volume uptdate the information available on established or newly described cancer genes, and may help manage the transition from basic research to clinical practice.

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Present and potential future applications of new diagnostic strategies basedon the direct or indirect detection of cancer genes are delineated in this volume. Among the methodological aspects covered are enzymatic target amplification by the polymerase chain reaction and related techniques, DNA fingerprinting, transfer of putative cancer genes in appropriate receipient cells, and recent developments inthe application of monoclonal antibodies in immunohistochemistry and immunoscintigraphy. The diagnostic and functional implications of mutations in cancer genes such as ras and p53 are described. The characterization of cancer genes and their products is correlated with growth control anddissemination of tumour cells by in vitro or clinical evidence. The contributions in the present volume uptdate the information available on established or newly described cancer genes, and may help manage the transition from basic research to clinical practice.
Content:
Front Matter....Pages I-IX
The Polymerase Chain Reaction: Methodology and Diagnostic Potential....Pages 1-16
Gene Loss as Marker in Tumor Development: Considerations of the Origin of Specific Brain Tumors Due to Combinant Loss of Function Mutations of Tumor Suppressor Genes....Pages 17-25
p53: Oncogene, Tumor Suppressor, or Both?....Pages 27-39
Tumor Genome Screening by Multilocus DNA Fingerprints as Obtained by Simple Repetitive Oligonucleotide Probes....Pages 41-51
Activation of ras Oncogenes in Human Tumours....Pages 53-64
Laboratory Techniques in the Investigation of Human Papillomavirus Infection....Pages 65-76
Detection of Minimal Residual Leukemia by Polymerase Chain Reactions....Pages 77-85
A Dominant Metastogene Confers Metastatic Potential to Tumor Cells....Pages 87-95
Progression in Human Melanoma Is Accompanied by the Altered Expression of Cell Adhesion Molecules....Pages 97-105
Structure, Function and Expression of the CEA Gene Family: Diagnostic and Therapeutic Implications....Pages 107-115
Membrane Proteins as Markers for Normal and Neoplastic Endocrine Cells....Pages 117-127
Biological and Clinical Relevance of the Tumor-Associated Serine Protease uPA....Pages 129-149
Analysis of Growth Regulatory Pathways in Human Neuro-Oncology....Pages 151-167
HER-2/Neu/c-ERB-B2 Amplification: Clinical Relevance in Mammary Carcinoma....Pages 169-174
Cytokine-Mediated Regulation of Growth Factor Receptors (EGF-R and erb-B2) in Pancreatic Tumors....Pages 175-186
Chimeric CEA-Specific Antibody Fragments and Anti-idiotypic Antibodies for Immunoscintigraphy and New Therapeutic Approaches to Colorectal Carcinomas....Pages 187-206
Tumor Localization by Immunoscintigraphy: Potential and Limitations....Pages 207-217
Back Matter....Pages 219-223

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Present and potential future applications of new diagnostic strategies basedon the direct or indirect detection of cancer genes are delineated in this volume. Among the methodological aspects covered are enzymatic target amplification by the polymerase chain reaction and related techniques, DNA fingerprinting, transfer of putative cancer genes in appropriate receipient cells, and recent developments inthe application of monoclonal antibodies in immunohistochemistry and immunoscintigraphy. The diagnostic and functional implications of mutations in cancer genes such as ras and p53 are described. The characterization of cancer genes and their products is correlated with growth control anddissemination of tumour cells by in vitro or clinical evidence. The contributions in the present volume uptdate the information available on established or newly described cancer genes, and may help manage the transition from basic research to clinical practice.
Content:
Front Matter....Pages I-IX
The Polymerase Chain Reaction: Methodology and Diagnostic Potential....Pages 1-16
Gene Loss as Marker in Tumor Development: Considerations of the Origin of Specific Brain Tumors Due to Combinant Loss of Function Mutations of Tumor Suppressor Genes....Pages 17-25
p53: Oncogene, Tumor Suppressor, or Both?....Pages 27-39
Tumor Genome Screening by Multilocus DNA Fingerprints as Obtained by Simple Repetitive Oligonucleotide Probes....Pages 41-51
Activation of ras Oncogenes in Human Tumours....Pages 53-64
Laboratory Techniques in the Investigation of Human Papillomavirus Infection....Pages 65-76
Detection of Minimal Residual Leukemia by Polymerase Chain Reactions....Pages 77-85
A Dominant Metastogene Confers Metastatic Potential to Tumor Cells....Pages 87-95
Progression in Human Melanoma Is Accompanied by the Altered Expression of Cell Adhesion Molecules....Pages 97-105
Structure, Function and Expression of the CEA Gene Family: Diagnostic and Therapeutic Implications....Pages 107-115
Membrane Proteins as Markers for Normal and Neoplastic Endocrine Cells....Pages 117-127
Biological and Clinical Relevance of the Tumor-Associated Serine Protease uPA....Pages 129-149
Analysis of Growth Regulatory Pathways in Human Neuro-Oncology....Pages 151-167
HER-2/Neu/c-ERB-B2 Amplification: Clinical Relevance in Mammary Carcinoma....Pages 169-174
Cytokine-Mediated Regulation of Growth Factor Receptors (EGF-R and erb-B2) in Pancreatic Tumors....Pages 175-186
Chimeric CEA-Specific Antibody Fragments and Anti-idiotypic Antibodies for Immunoscintigraphy and New Therapeutic Approaches to Colorectal Carcinomas....Pages 187-206
Tumor Localization by Immunoscintigraphy: Potential and Limitations....Pages 207-217
Back Matter....Pages 219-223
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