Ebook: Fine Structure Immunocytochemistry
Author: Dr. Gareth Griffiths (auth.)
- Tags: Cell Biology, Biochemistry general
- Year: 1993
- Publisher: Springer-Verlag Berlin Heidelberg
- Edition: 1
- Language: English
- pdf
Electron microscopy in the biological sciences can be divided into two disciplines. The first, concerned with high resolution detail of particles or periodic structures, is mostly based on sound theoretical principles of physics. The second, by far the larger discipline, is interested in the information obtainable from thin sections. The theoretical back ground to those groups of techniques for preparing and looking at thin sections is often inexact and "loose", for want of a better word. What should be chemistry is often closer to alchemy. This kind of electron microscopy is often enshrined with mystical recipes, handed down from generation to generation. Admittedly, many of the processes involved, such as those required to embed tissue in epoxy resins, involve multiple interconnected steps, which make it difficult to follow the details of anyone of these steps. If all these steps are shrouded in some mystery, however, can one really trust the final image that emerges on the EM screen? When we present the data in some semi quantitative form is there really no better way to do it than to categorize the parameters with ++, +/-, etc? What happens when one labels the sections with antibodies? Does the whole business necess arily need to be more of an "art" than a "science"? Upon reflecting on these problems in 1981, I had the impression that many of the multi-authored textbooks that existed then (and that have appeared since) tended to exacerbate or at least perpetuate this
Content:
Front Matter....Pages I-XXI
Introduction to Immunocytochemistry and Historical Background....Pages 1-8
Fine-Structure Preservation....Pages 9-25
Fixation for Fine Structure Preservation and Immunocytochemistry....Pages 26-89
Embedding Media for Section Immunocytochemistry....Pages 90-136
Cryo and Replica Techniques for Immunolabelling....Pages 137-203
Elementary Immunology....Pages 204-236
Labelling Reactions for Immunocytochemistry....Pages 237-278
Particulate Markers for Immunoelectron Microscopy....Pages 279-306
Non-Immunological High-Affinity Interactions Used for Labelling....Pages 307-344
Preembedding Immuno-Labelling....Pages 345-370
Quantitative Aspects of Immunocytochemistry....Pages 371-445
An Overview of Techniques for Labelling at the EM Level....Pages 446-449
Back Matter....Pages 451-459
Content:
Front Matter....Pages I-XXI
Introduction to Immunocytochemistry and Historical Background....Pages 1-8
Fine-Structure Preservation....Pages 9-25
Fixation for Fine Structure Preservation and Immunocytochemistry....Pages 26-89
Embedding Media for Section Immunocytochemistry....Pages 90-136
Cryo and Replica Techniques for Immunolabelling....Pages 137-203
Elementary Immunology....Pages 204-236
Labelling Reactions for Immunocytochemistry....Pages 237-278
Particulate Markers for Immunoelectron Microscopy....Pages 279-306
Non-Immunological High-Affinity Interactions Used for Labelling....Pages 307-344
Preembedding Immuno-Labelling....Pages 345-370
Quantitative Aspects of Immunocytochemistry....Pages 371-445
An Overview of Techniques for Labelling at the EM Level....Pages 446-449
Back Matter....Pages 451-459
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