Ebook: Methods in Protein Sequence Analysis

The Ninth International Conference on Methods in Protein Sequence Analysis was held for the first time in Asia from September 20 to September 24, 1992 in Otsu (a city near Kyoto), Japan. Approximately 400 delegates attended the meeting. Forty papers were presented orally and 147 poster presentations were discussed. Academic sessions were held from early in the morning until late in the evening. We are confident that the Conference was successful in providing up-to-date information about methods in protein sequence analysis to all participants. Moreover, with the knowledge and understanding of the present standard of various methods of analysis that are being used and will be used, we were able to clarify areas that need to be evaluated, to be improved and be explored further. Major topics in the Conference mostly covered areas in the methodology of protein sequence analysis, such as: micropreparation and microsequencing of proteins, mass spectrometry, post-translational modification, prediction and database analysis, and analysis of protein structures of special interests. The evolution of genetic engineering in molecular biology has greatly accelerated the accumulation of knowledge on the amino acid sequence of novel proteins regardless of whether they are expressed or not expressed in living organisms. In the early stage of accumulation of structural information, the amino acid sequence itself is worthy of notice.

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Content:
Front Matter....Pages i-xvi
Front Matter....Pages 1-1
Capillary Liquid Chromatography: A Tool for Protein Structural Analysis....Pages 3-10
Routine Amino Acid Sequencing on 2D-Gel Separated Proteins: A Protein Elution and Concentration Gel System....Pages 11-19
Capillary Electrophoresis in Structural Characterization of Polypeptides....Pages 21-28
Sensitization of Gas-Phase Protein Sequencer using Fluorescein Isothiocyanate (FITC)....Pages 29-36
Thiobenzoylation Method of Protein Sequencing: Gas Chromatography/Mass Spectrometric Detection of 5-Acetoxy-2-Phenylthiazoles....Pages 37-44
Deblocking and Subsequent Microsequence Analysis of N-Terminally Blocked Proteins Immobilized on PVDF Membrane....Pages 45-53
Development of Novel C-Terminal Sequencing Methods....Pages 55-62
Automated C-Terminal Sequencing of Peptides and Proteins....Pages 63-69
A New Chemical Approach to C-Terminal Microsequence Analysis via the Thiohydantoin....Pages 71-78
Cyanogen Bromide Cleavage of Proteins on Blots and Subsequent Separation of the Fragments by Polyacrylamide Gel Electrophoresis Directly from those Blots....Pages 79-85
Identification of the Disulfide Bonds of the Human Complement Component C3....Pages 87-93
A Novel Protease from Jack-Bean Seeds: Asparaginyl Endopeptidase....Pages 95-100
Lysine-Specific Serine Protease from Achromobacter Lyticus: Its Substrate Specificity and Comparison with Trypsin....Pages 101-106
Structure and Function of Snake Venom Metalloproteinase Family....Pages 107-115
Front Matter....Pages 117-117
Recent Advances in Protein Sequencing by Mass Spectrometry Introduction and Overview....Pages 119-126
Sequence Analysis of Peptides Presented to the Immune System by Class I and Class II MHC Molecules....Pages 127-133
High Precision Mass Spectrometry with the Ion Trap Mass Spectrometer....Pages 135-141
Electrospray-Mass Spectrometry, an Emerging Methodology for Elucidating Structure-Function Relationships of Proteins....Pages 143-147
A General Strategy for the use of Mass Spectrometric Molecular Weight Information in Protein Purification and Sequence Determination....Pages 149-156
Determination of Posttranslational Modifications by Mass Spectrometry....Pages 157-164
Front Matter....Pages 165-165
Post-Translational Modifications of Proteins....Pages 167-172
Posttranslational Glutamylation of Several Brain Tubulin Isotypes: Structure of the Polyglutamyl Side-Chain....Pages 173-181
Protein Anchoring to Membrane by Glycosylphosphatidylinositol. Determination of the COOH-Terminal Signal Peptide Sequence and GPI-Attachment Site in Bovine Liver 5?-Nucleotidase....Pages 183-190
Pyridylamination for Sensitive Analysis of Sugar Chains of Glycoproteins....Pages 191-194
Sequence Analysis of Phosphopeptides and its Application for the Determination of Phosphorylated Sites of Proteins....Pages 195-198
Front Matter....Pages 199-206
Prediction of Protein Structure from Multiple Sequence Alignment....Pages 207-207
Sequence Patterns that Characterize Protein Families with a Common Fold....Pages 209-220
Domains and Modules of Proteins....Pages 221-227
Homology Search and Prediction of Biological Function of Protein from Amino Acid Sequences....Pages 229-232
Amino Acid Sequence Comparison as an Aid to Determining Evolutionary Origins....Pages 233-240
Protein Databases Constructed by Quantitative Two-Dimensional Gel Electrophoresis....Pages 241-246
Front Matter....Pages 247-253
Biochemistry of a Natriuretic Peptide Family....Pages 255-255
Activin, Activin-Binding Protein (Follistatin) and Activin Receptor....Pages 257-260
Do Well-Populated Intermediates Represent the Pathway of Protein Folding?....Pages 261-268
Alcohol Dehydrogenases: Patterns of Protein Evolution....Pages 269-273
Structure and Post-Translational Modification of the Lipoyl Domain of 2-Oxo Acid Dehydrogenase Complexes: A New Family of Protein Domains....Pages 275-282
Structure Analysis of the Topography and Molecular Organization of Protein-RNA Complexes as Revealed in Ribosomes....Pages 283-288
Back Matter....Pages 289-306
....Pages 307-310