Ebook: The Release of Genetically Modified Microorganisms—REGEM 2

If ripple effect is a measure of greatness in scientific discovery then GEMMOs have a lot going for them and this book dramatically illustrates the risks associated with advances being made by researchers to mobilize and control the power of the microorganism in the world's fight to perfect nature and fmd remedies for its imperfections. In the field of genetic science it is abundantly clear that so much more can be achieved through prevention rather than cure and that the indirect kill, by reason of its logic is a much more powerful weapon for winning results. Nevertheless the dilemma facing politicians arises over whether man should tamper with something which is God-given such as Radioactivity and Genetic endowment. The Roman Catholic church fmds difficulty in accepting the proposition that what is God­ given can be treated as a product under human control and maybe that is why recently half a century of genetic research on a strain of bees resistant to a devastating parasite at the Buckfastleigh Benedictine Monastery has inexplicably ceased whilst verging on scientific success.(l) The Anglican Community on the other hand does not see the sacrosanctity of Radioactivity and Genetic material as a bar to man-manipulation with appropriate safeguards.

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Content:
Front Matter....Pages i-xiv
Environmental Pressure Imposed on Gemmos in Soil....Pages 1-14
Interactions in Communities of Microorganisms....Pages 15-24
Application of Genetically-Modified Microorganisms in Agriculture....Pages 25-31
Bioremediation and Waste Management....Pages 33-45
Progress in the Genetic Modification and Field-Release of Baculovirus Insecticides....Pages 47-58
Exploitation of Genetically-Modified Microorganisms in the Food Industry....Pages 59-69
Live Attenuated Salmonellae: Oral Vaccines for Salmonellosis and Combined Vaccines Carrying Heterologous Antigens....Pages 71-83
Commercial Use of Microbial Inocula Containing Live Genetically Modified Microorganisms (GEMMOs)....Pages 85-92
Risk Assessment....Pages 93-98
Social Implications and Public Confidence: Risk Perception and Communication....Pages 99-114
Workshop 1 Persistence and Survival of Genetically-Modified Microorganisms Released into the Environment....Pages 115-118
Workshop 2 Detection Methods for Modified Organisms in the Environment....Pages 119-122
Workshop 3 Environmental Impact....Pages 123-127
Characterization of Microbial Emissions from a Fermentation Plant Using a Genetically-Modified Bacillus Strain....Pages 129-131
Transfer of Inc.P and Inc.Q Plasmids in Soil and Filter Matings from Pseudomonas Fluorescens to a Recipient Strain and to Indigenous Bacteria....Pages 133-135
Impact of Introducing Genetically-Modified Microorganisms on Soil Microbial Community Diversity....Pages 137-139
The Detection and Survival of Recombinant Pseudomonas Putida Populations in Lake Water....Pages 141-143
Survival of Introduced Bacteria in Rhizosphere and Non-Rhizosphere Soils....Pages 145-147
Implementation of Pre-Tests for Gemmo Release in the Environment....Pages 149-151
Plasmid Transfer and Stability of Pseudomonas Cepacia and Enterobacter Cloacae in a Continuous Flow Culture System....Pages 153-153
Risk Assessment in Releases of Nitrogen-Fixing Enterobacter into Soil; Survival and Gene Transfer, as Influenced by Agricultural Substrates....Pages 155-156
Direct Detection of E.Agglomerans DNA Sequences in Soil....Pages 157-159
The Survival of Microorganisms in the Open Air....Pages 161-162
Survival and Dispersal of Gemmos Associated with Animals....Pages 163-165
Study of Intestinal Colonization with Norfloxacin in Vivo and in Vitro....Pages 167-169
Studies on Gene Flux by Free Bacterial DNA in Soil, Sediment and Groundwater Aquifer....Pages 171-174
Plasmid Transfer Between Pseudomonas Aeruginosa Strains in Natural Soil....Pages 175-177
Investigation of Possible Gene Transfer from Genetically Modified to Indigenous Bacteria in Soil....Pages 179-180
Fate of Plant Pathogenic Pseudomonads in Bean Microcosms....Pages 181-182
Detection of Streptomyces Marker Plasmids in Soil....Pages 183-185
The Removal and Dispersal of Foliar Bacteria by Rain Splash....Pages 187-189
Growth and Survival of Genetically-Modified Pseudomonas Putida in Soils of Different Texture....Pages 191-193
Simplified Subtraction-Hybridization System for Isolation Of Strain-Specific Rhizobium DNA Probes....Pages 195-196
Plasmid Transfer of pBR322 Derivatives from recA’E. Coli K12 Donor Strain to Various Natural Gram-Negative Isolates....Pages 197-198
Single Cell Detection of Bioluminescent Pseudomonas Syringae in Soil....Pages 199-202
Luminescence-Based Detection to Assess Survival and Activity of a Genetically-Modified Inoculum in Soil....Pages 203-205
The Effect of Cyanobacterial Water Bloom Formation Upon Conjugational Gene Transfer Between Associated Heterotrophic Bacteria....Pages 207-208
Construction and Detection of Bioluminescent Bacillus Subtilis Strains....Pages 209-212
Detection of Lux-Gene Sequences in Escherichia Coli MM294 Extracted From Soil Using the Polymerase Chain Reaction and Gene Probing....Pages 213-215
Monitoring Survival of Genetically-Modified Rhizobium in the Field....Pages 217-219
Quantitative Detection of Genetically-Modified Micro Organisms by the PCR-MPN Method....Pages 221-223
Bioluminescent REC A Mutants of Rhizobium as Model Organisms in Risk Assessment Studies....Pages 225-227
An Artificial Microcosm to Study Microbial Interactions in the Rhizosphere....Pages 229-230
Detection of Bacteria from Natural Environments by Flow Cytometry....Pages 231-232
The Detection of Bacteria in Aquatic Environments by Flow Cytometry....Pages 233-234
Plasmid Transfer in Sterile Soil as Influenced by a Non-Simultaneous Inoculation of Donor and Recipient....Pages 235-236
Survival of Enterobacter Cloacae on Leaves and in Soil Detected by Immunofluorescence Microscopy in Comparison with Selective Plating....Pages 237-240
Genetic Manipulation of Rumen Bacteria....Pages 241-244
Detecting Natural Transformation of Acinetobacter Calcoaceticus, In Situ, Within Natural Epilithon Of The River Taff....Pages 245-247
The Survival of a Chloramphenicol Resistance Plasmid in a Natural Bacillus Population....Pages 249-252
What Values in the GEMMOs? Reflections on Regem 2....Pages 253-255
Back Matter....Pages 257-259
....Pages 261-265