Ebook: Bacteriocins, Microcins and Lantibiotics

Presented here are recent findings on bacteriocins: plasmid-encoded toxins produced by bacteria, which differ from traditional antibiotics in killing only bacteria that are closely related to the producing strain. Included are introductory chapters on bacteriocins, microcins (low molecular weight bacteriocins), and lantibiotics (peptide antibiotics containing lanthionine), further, contributions on pore forming bacteriocins, the mechanisms of immunity to bacteriocins, uptake and secretion, as well as evolution of bacteriocins. It is of particular interest that the lantibiotic nisin is approved for use as food preservative and another lantibiotic, epidermin, has potential as a therapeutic drug against acne.

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Presented here are recent findings on bacteriocins: plasmid-encoded toxins produced by bacteria, which differ from traditional antibiotics in killing only bacteria that are closely related to the producing strain. Included are introductory chapters on bacteriocins, microcins (low molecular weight bacteriocins), and lantibiotics (peptide antibiotics containing lanthionine), further, contributions on pore forming bacteriocins, the mechanisms of immunity to bacteriocins, uptake and secretion, as well as evolution of bacteriocins. It is of particular interest that the lantibiotic nisin is approved for use as food preservative and another lantibiotic, epidermin, has potential as a therapeutic drug against acne.
Content:
Front Matter....Pages I-XI
Introduction to the Microcin Session....Pages 1-2
Uptake and Mode of Action of the Peptide Antibiotic Microcin B17....Pages 3-13
The Structure and Maturation Pathway of Microcin B17....Pages 15-17
Bacteriocins of Gram-Positive Bacteria: An Opinion Regarding their Nature, Nomenclature and Numbers....Pages 19-31
Molecular Properties of Lactobacillus Bacteriocins....Pages 33-35
Lactococcal Bacteriocins: Genetics and Mode of Action....Pages 37-58
Introduction to the Lantibiotics session....Pages 59-69
Lantibiotics : An Overview and Conformational Studies on Gallidermin and Pep5....Pages 71-73
Biosynthesis of the Lantibiotic Pep5 and Mode of Action of Type A Lantibiotics....Pages 75-92
Identification of Genes Involved in Lantibiotic Biosynthesis....Pages 93-106
Pore Forming Bacteriocins....Pages 107-116
Site-Directed Fluorescence Spectroscopy as a Tool to Study the Membrane Insertion of Colicin A....Pages 117-118
Structure-Function of the Colicin E1 Ion Channel: Voltage-Driven Translocation and Gating of a Tetra- (or Hexa-) Helix Channel....Pages 119-125
Voltage-Dependent Gating of Colicin E1 Channels in Planar Bilayers....Pages 127-138
Immunity to Colicins....Pages 139-150
Immunity Protein to Pore Forming Colicins....Pages 151-162
Specificity Determinants of the Interaction Between Colicin E9 and its Immunity Protein....Pages 163-170
Structural studies on colicin E3 and its immunity protein....Pages 171-179
Study of the Import Mechanisms of Colicins Through Protein Engineering and K+ Efflux Kinetics....Pages 181-201
Import and Export of Colicin M....Pages 203-214
TolA: Structure, Location and Role in the Uptake of Colicins....Pages 215-223
Domains of the Escherichia Coli BtuB Protein Involved in Outer Membrane Association and Interaction with Colicin Translocation Components....Pages 225-242
A Structure-Function Analysis of BtuB, the E.Coli. Vitamin B12 Outer Membrane Transport Protein....Pages 243-253
General Introduction to the Secretion of Bacteriocins....Pages 255-269
Functioning of the pCLODF13 Encoded BRP....Pages 271-296
Structure/Function Relationships in the Signal Sequence of the Colicin A Lysis Protein....Pages 297-305
The Secretion of Colicin V....Pages 307-316
Introduction to the Session on the Evolution of Bacteriocins....Pages 317-329
Molecular evolution of E colicin plasmids with emphasis on the endonuclease types....Pages 331-348
Immunity Specificity and Evolution of the Nuclease-Type E Colicins....Pages 349-351
Replicon Evolution of ColE2-related Plasmids....Pages 353-378
Genetic Determinants for Microcin H47, an Escherichia Coli Chromosome-Encoded Antibiotic....Pages 379-395
BLIS Production in the Genus Streptococcus....Pages 397-411
Cloning and Characterisation of a Lysin Gene from a Listeria Bacteriophage....Pages 413-415
Transformation of Enterococcus faecalis OGIX with the Plasmid pMB2 Encoding for the Peptide Antibiotic AS-48, by Protoplast Fusion and Regeneration on Calcium Alginate....Pages 417-420
NMR Studies of Lantibiotics: The Three-Dimensional Structure of Nisin in Aqueous Solution....Pages 421-426
Localization and Phenotypic Expression of Genes Involved in the Biosynthesis of the Lactococcus Lactis subsp. Lactis Lantibiotic Nisin....Pages 427-431
Tn5301, A Lactococcal Transposon Encoding Genes for Nisin Biosynthesis....Pages 433-434
Development of Yeast Inhibitory Compounds for Incorporation into Silage Inoculants....Pages 435-447
The excC and excD genes of Escherichia coli K-12 encode the peptidoglycan-associated lipoprotein (PAL) and the TolQ protein, respectively....Pages 449-461
Resistance and Tolerance of Bacteria to E Colicins....Pages 463-472
Construction and Characterization of Chimeric Proteins Between Pyocins and Colicin E3....Pages 473-477
Colicins as Anti-tumour Drugs....Pages 479-485
Back Matter....Pages 487-491
....Pages 493-502