Ebook: The Photosynthetic Bacterial Reaction Center II: Structure, Spectroscopy and Dynamics

The NATO Advanced Research Workshop entitled "The Photosynthetic Bacterial Reaction Center: Structure, Spectroscopy, and Dynamics" was held May 10-15, 1992, in the Maison d'H6tes of the Centre d'Etudes Nuc1eaires de Cadarache near Aix-en-Provence in the south of France. This workshop is the most recent of a string of meetings which started in Feldafing (Germany) in March 1985, soon after the three-dimensional structure of the bacterial reaction center had been elucidated by X-ray crystallography. This was followed, in September 1987, by a workshop in Cadarache and, in March 1990, by a second meeting in Feldafing. Although one of the most important processes on Earth, photosynthesis is still poorly understood. Stimulated by the breakthrough of solving the bacterial reaction center structure at atomic resolution, the field of relating this structure to the function of the reaction center, i. e. the remarkably efficient conversion and storage of solar energy, has been developing vigorously. Once the general organization of the cofactors and some details of the protein-cofactor interactions were known, it became possible to combine a variety of spectroscopic techniques with the powerful tool of site-directed mutagenesis in order to address increasingly incisive questions about the specific role of some amino acid residues in the electron transfer process. Still another promising tool is being developed, namely the exchange of a number of the native bacteriochlorophyll and bacteriopheophytin cofactors by chemically modified pigments.

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Symmetrical InterSubunit Suppressors of the Bacterial Reaction Center cdhelix Exchange Mutants; S.J. Robles, et al. Suggestions for Directed Engineering of Reaction Centers; J. Fajer, et al. Potential Energy Function for Photosynthetic Reaction Center Chromophores; N. Foloppe,et al. Bacterial Reaction Centers with Planttype Pheophytins; H.Scheer, et al. EPR and ENDOR Studies of the Primary Donor Cation Radical in Native and Genetically Modified Bacterial Reaction Centers; J. Rautter, et al. Molecular Orbital Study of Electronic Asymmetry in Primary Donors of Bacterial Reaction Centers; M. Plato, et al. LightInduced Charge Separation in Photosynthetic Bacterial Reaction Centers Monitored by FTIR Difference Spectroscopy; J. Breton, et al. Effect of Charge Transfer States on the Zero Phonon Line of the Special Pair in the Bacterial Reaction Center; E.J.P. Lathrop, R.A.Friesner. 38 additional articles. Index.

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Symmetrical InterSubunit Suppressors of the Bacterial Reaction Center cdhelix Exchange Mutants; S.J. Robles, et al. Suggestions for Directed Engineering of Reaction Centers; J. Fajer, et al. Potential Energy Function for Photosynthetic Reaction Center Chromophores; N. Foloppe,et al. Bacterial Reaction Centers with Planttype Pheophytins; H.Scheer, et al. EPR and ENDOR Studies of the Primary Donor Cation Radical in Native and Genetically Modified Bacterial Reaction Centers; J. Rautter, et al. Molecular Orbital Study of Electronic Asymmetry in Primary Donors of Bacterial Reaction Centers; M. Plato, et al. LightInduced Charge Separation in Photosynthetic Bacterial Reaction Centers Monitored by FTIR Difference Spectroscopy; J. Breton, et al. Effect of Charge Transfer States on the Zero Phonon Line of the Special Pair in the Bacterial Reaction Center; E.J.P. Lathrop, R.A.Friesner. 38 additional articles. Index.
Content:
Front Matter....Pages i-x
Correlation Between the Polarized Light Absorption and the X-Ray Structure of Single Crystals of the Reaction Center from Rhodobacter Sphaeroides R-26....Pages 1-11
Symmetrical Inter-Subunit Suppressors of the Bacterial Reaction Center cd-Helix Exchange Mutants....Pages 13-20
Suggestions for Directed Engineering of Reaction Centers: Metal, Substituent and Charge Modifications....Pages 21-23
Potential Energy Function for Photosynthetic Reaction Centre Chromophores: Energy Minimisations of a Crystalline Bacteriopheophytin a Analog....Pages 25-31
Bacterial Reaction Centers with Plant-Type Pheophytins....Pages 33-42
Trapping of a Stable Form of Reduced Bacteriopheophytin and Bacteriochlorophyll in Ectothiorhodospira SP. Photoreaction Center....Pages 43-48
Triplet-Minus-Singlet Absorbance Difference Spectroscopy of Heliobacterium Chlorum Monitored with Absorbance-Detected Magnetic Resonance....Pages 49-57
Mid- and Near-IR Electronic Transitions of P+: New Probes of Resonance Interactions and Structural Asymmetry in Reaction Centers....Pages 59-65
EPR and Endor Studies of the Primary Donor Cation Radical in Native and Genetically Modified Bacterial Reaction Centers....Pages 67-77
Molecular Orbital Study of Electronic Asymmetry in Primary Donors of Bacterial Reaction Centers....Pages 79-88
Near-Infrared-Excitation Resonance Raman Studies of Bacterial Reaction Centers....Pages 89-97
Asymmetric Structural Aspects of the Primary Donor in Several Photosynthetic Bacteria: the Near-IR Fourier Transform Raman Approach....Pages 99-108
FTIR Characterization of Leu M160>His, Leu L131>His and His L168>Phe Mutations Near the Primary Electron Donor in RB. Sphaeroides Reaction Centers....Pages 109-118
Light-Induced Charge Separation in Photosynthetic Bacterial Reaction Centers Monitored by FTIR Difference Spectroscopy: the QA Vibrations....Pages 119-126
Time-Resolved Infrared and Static FTIR Studies of QA > QB Electron Transfer in Rhodopseudomonas Viridis Reaction Centers....Pages 127-132
Is Dispersive Kinetics from Structural Heterogeneity Responsible for the Nonexponential Decay of P870* in Bacterial Reaction Centers?....Pages 133-139
Effect of Charge Transfer States on the Zero Phonon Line of the Special Pair in the Bacterial Reaction Center....Pages 141-145
Recent Experimental Results for the Initial Step of Bacterial Photosynthesis....Pages 147-153
Femtosecond Spectroscopy of the Primary Electron Transfer in Photosynthetic Reaction Centers....Pages 155-162
Electron Transfer in Rhodopseudomonas Viridis Reaction Centers with Prereduced Bacteriopheophytin BL....Pages 163-172
Fast Internal Conversion in Bacteriochlorophyll Dimers....Pages 173-181
Primary Charge Separation in Reaction Centers: Time-Resolved Spectral Features of Electric Field Induced Reduction of Quantum Yield....Pages 183-192
Electric Field Effects on the Quantum Yields and Kinetics of Fluorescence and Transient Intermediates in Bacterial Reaction Centers....Pages 193-207
Radical Pair Dynamics in the Bacterial Photosynthetic Reaction Center....Pages 209-217
The Primary Charge Separation in Bacterial Photosynthesis. What is New?....Pages 219-225
Multi—Mode Coupling of Protein Motion to Electron Transfer in the Photosynthetic Reaction Center: Spin — Boson Theory Based on a Classical Molecular Dynamics Simulation....Pages 227-235
Charges Recombination Kinetics in Bacterial Photosynthetic Reaction Centers: Conformational States in Equilibrium Pre-Exist in the Dark....Pages 237-243
Protein Relaxation Following Quinone Reduction in Rhodobacter Capsulatus: Detection of Likely Protonation-Linked Optical Absorbance Changes of the Chromophores....Pages 245-251
Study of Reaction Center Function by Analysis of the Effects of Site-Specific and Compensatory Mutations....Pages 253-260
Proton Transfer Pathways in the Reaction Center of Rhodobacter Sphaeroides: A Computational Study....Pages 261-270
Initial Characterization of the Proton Transfer Pathway to QB in Rhodopseudomonas Viridis: Electron Transfer Kinetics in Herbicide-Resistant Mutants....Pages 271-282
Study of Reaction Centers From RB. Capsulatus Mutants Modified in the QB Binding Site....Pages 283-290
Calculations of Proton Uptake in Rhodobacter Sphaeroides Reaction Centers....Pages 291-300
Chlorophyll Triplet States in the CP47-D1-D2-Cytochrome b-559 Complex of Photosystem II....Pages 301-312
Light Reflections II....Pages 313-319
Back Matter....Pages 321-330
....Pages 331-339